Complete genome sequence of the sulfate-reducing Firmicute Desulfotomaculum ruminis type strain (DLT)

Desulfotomaculum ruminis Campbell and Postgate 1965 is a member of the large genus Desulfotomaculum which contains 30 species and is contained in the family Peptococcaceae. This species is of interest because it represents one of the few sulfate-reducing bacteria that have been isolated from the rumen. Here we describe the features of D. ruminis together with the complete genome sequence and annotation. The 3,969,014 bp long chromosome with a total of 3,901 protein-coding and 85 RNA genes is the second completed genome sequence of a type strain of the genus Desulfotomaculum to be published, and was sequenced as part of the DOE Joint Genome Institute Community Sequencing Program 2009

Сравнительная характеристика пневмоний, вызванных Mycoplasma pneumoniae, у детей

The urgency of the problem of community-acquired pneumonia in children is due to the high incidence rate. In the etiological structure of bacterial pneumonia, Streptococcus pneumoniae and Mycoplasma pneumonia (Mp) prevail.Purpose: to identify clinical, laboratory and instrumental features of mycoplasmal pneumonia in children.Materials and methods. A retrospective,  single-center cohort study  of  266  case  histories  of  children  aged  4 months to 17 years who were in Children’s City Clinical Hospital No. 9 in 2019 with a referral diagnosis of pneumonia was carried out. To verify the diagnosis of pneumonia, the method of chest X-ray was used, for the etiological diagnosis, the method of PCR swabs from the nasopharynx, ELISA for the detection of antibodies of the IgM and IgG classes was used. Results. The diagnosis  of  pneumonia was confirmed in 190 children. The diagnosis of pneumonia caused by M. pneumoniae (MpP) was established in 76 (40%) children, they made up the 1st group. The remaining 114 (60%) children with community-acquired pneumonia of another etiology (CAP) made up the 2nd group – comparisons. The diagnosis of MpP was confirmed in 46 (60.5%) children by the detection of IgM, in 12 (15.8%) by the detection of Mp genetic material, and in  18  (23.7%) by positive both IgM and PCR. The median age of children in the group with MpP was 9.6 years, in the comparison group – 4 years (p<0.01). Significantly more often MpP occurs in children aged 11–17 years (p < 0.01), and CAP – up to 7 years (p < 0.01). With MpP, catarrhal phenomena in the form of hyperemia of the oropharynx, nasal congestion, and unproductive cough are observed less frequently than with CAP. With MpP, rales in the lungs are heard more often than with CAP, they are significantly more often wet (p<0.01). Respiratory failure and dyspnea are less common in MpP than in CAP. Bilateral lesions and lesions of the upper lobe of the lungs are more often detected in MpP, and in CAP – right-sided lesions. In the general blood test, leukocytosis, incl. above 15  thousand/µl,  as well as leukopenia below 4 thousand/µl were significantly more frequent in the group of patients with CAP (p<0.01). Conclusion. No clear clinical and laboratory criteria for mycoplasmal etiology of pneumonia have been obtained, which dictates the need for laboratory confirmation for the choice of therapy tactics.Актуальность проблемы внебольничных пневмоний у детей обусловлена высоким  уровнем  заболеваемости. В этиологической структуре бактериальных пневмоний превалируют Streptococcus pneumoniae и Mycoplasma pneumoniae.Цель: выявление клинико-лабораторных и инструментальных особенностей микоплазменной пневмонии у детей.Материалы и методы: проведено ретроспективное, одноцентровое когортное исследование 266 историй болезни детей в возрасте от 4 месяцев до 17 лет, находившихся в Детской городской клинической больнице № 9 в 2019 г. с направительным диагнозом «Пневмония». Для верификации диагноза пневмонии использовали рентгенографию грудной клетки, для этиологического диагноза – ПЦР мазков из носоглотки, ИФА для обнаружения антител класса IgM и IgG.Результаты: диагноз «Пневмония» был подтвержден у 190 детей. Микоплазменная пневмония (MpП) была установлена у 76 (40%) детей, они составили 1-ю группу (у 46 (60,5%) детей обнаружены IgM, у 12 (15,8%) – положительная ПЦР, а у 18 (23,7%) – положительные и IgM, и ПЦР). Остальные 114 (60%) детей с внебольничной пневмонией другой этиологии (ВП) составили 2-ю группу – сравнения. Медиана возраста детей в группе с МрП составила 9,6 лет, в группе сравнения – 4 года (р<0,01). Достоверно чаще MpП встречается у детей в возрасте 11–17 лет (р < 0,01), а ВП – до 7 лет (р < 0,01). При MpП катаральные явления в виде гиперемии ротоглотки, заложенности носа, малопродуктивного кашля наблюдаются реже, чем при ВП. При MpП чаще выслушиваются хрипы в легких, чем при ВП, достоверно чаще они влажные (р<0,01). При MpП дыхательная недостаточность и одышка наблюдаются реже, чем при ВП. Поражение верхней доли легких чаще выявляется при MpП, а при ВП – правостороннее поражение. В общем анализе крови лейкоцитоз, в том числе выше 15 тыс./мкл, а также лейкопения ниже 4 тыс./мкл были достоверно чаще в группе больных с ВП (р<0,01).Заключение: не получено четких клинико-лабораторных критериев пневмонии микоплазменной этиологии, что диктует необходимость лабораторного подтверждения этиологии пневмонии для выбора тактики терапии

Recovery of dialysis patients with COVID-19 : health outcomes 3 months after diagnosis in ERACODA

Background. Coronavirus disease 2019 (COVID-19)-related short-term mortality is high in dialysis patients, but longer-term outcomes are largely unknown. We therefore assessed patient recovery in a large cohort of dialysis patients 3 months after their COVID-19 diagnosis. Methods. We analyzed data on dialysis patients diagnosed with COVID-19 from 1 February 2020 to 31 March 2021 from the European Renal Association COVID-19 Database (ERACODA). The outcomes studied were patient survival, residence and functional and mental health status (estimated by their treating physician) 3 months after COVID-19 diagnosis. Complete follow-up data were available for 854 surviving patients. Patient characteristics associated with recovery were analyzed using logistic regression. Results. In 2449 hemodialysis patients (mean ± SD age 67.5 ± 14.4 years, 62% male), survival probabilities at 3 months after COVID-19 diagnosis were 90% for nonhospitalized patients (n = 1087), 73% for patients admitted to the hospital but not to an intensive care unit (ICU) (n = 1165) and 40% for those admitted to an ICU (n = 197). Patient survival hardly decreased between 28 days and 3 months after COVID-19 diagnosis. At 3 months, 87% functioned at their pre-existent functional and 94% at their pre-existent mental level. Only few of the surviving patients were still admitted to the hospital (0.8-6.3%) or a nursing home (∼5%). A higher age and frailty score at presentation and ICU admission were associated with worse functional outcome. Conclusions. Mortality between 28 days and 3 months after COVID-19 diagnosis was low and the majority of patients who survived COVID-19 recovered to their pre-existent functional and mental health level at 3 months after diagnosis

PSEUDOHYPOPARATHYROIDISM Ia TYPE WITH EARLY DEBUT IN SISTERS OF ONE FAMILY

Pseudohypoparathyroidism is a rare genetic disorder characterised by end-organ resistance to parathyroid hormone due to a defect of the guanine nucleotide-binding protein alpha that simulates activity of the polypeptide 1 (GNAS) gene. Patients with type Ia pseudohypoparathyroidism have different features of Albright's hereditary osteodystrophy and characteristic phenotype (obesity, round face, short stature, short neck, brachidactyly, etc.), multi-hormone resistance. We describe two sisters (half sibs), who presented with different symptoms of pseudohypoparathyroidism and clinically manifested different degree of Albright's hereditary osteodystrophy. Genetic study detected a mutation p.D190MfsX14 (c.568 571 delGACT), in theGNAS 1 gene (OMIM*139320)

ENDOTHELIAL DYSFUNCTION AND BLOOD VISCOSITY INPATIENTS WITH UNSTABLE ANGINA IN DIFFERENT PERIODS OF A SOLAR ACTIVITY

The origin of hemorheologic and endothelial defects in patients with unstable angina (comparing with healthy persons) is determined by a solar activity period: the blood viscosity increases in a period of high solar activity in the vessels of small, medium and macro diameters, a local decompensate dysfunction of small vessels endothelium had been fixed (microcirculation area). In the period of a low solar activity there is an increase of a blood viscosity in vessels of all diameters, generalized subcompensated endothelial dysfunction is developed (on the background of the III phase blood clotting activating). In the period of a high solar activity a higher blood viscosity had been fixed, comparing with the period of a low solar activity. 

H2 enrichment from synthesis gas by Desulfotomaculum carboxydivorans for potential applications in synthesis gas purification and biodesulfurization

9 páginas, 5 figuras, 1 tabla.Desulfotomaculum carboxydivorans, recently isolated from a full-scale anaerobic wastewater treatment facility, is a sulfate reducer capable of hydrogenogenic growth on carbon monoxide (CO). In the presence of sulfate, the hydrogen formed is used for sulfate reduction. The organism grows rapidly at 200 kPa CO, pH 7.0, and 55°C, with a generation time of 100 min, producing nearly equimolar amounts of H2 and CO2 from CO and H2O. The high specific CO conversion rates, exceeding 0.8 mol CO (g protein)−1 h−1, makes this bacterium an interesting candidate for a biological alternative of the currently employed chemical catalytic water–gas shift reaction to purify synthesis gas (contains mainly H2, CO, and CO2). Furthermore, as D. carboxydivorans is capable of hydrogenotrophic sulfate reduction at partial CO pressures exceeding 100 kPa, it is also a good candidate for biodesulfurization processes using synthesis gas as electron donor at elevated temperatures, e.g., in biological flue gas desulfurization. Although high maximal specific sulfate reduction rates (32 mmol (g protein)−1 h−1) can be obtained, its sulfide tolerance is rather low and pH dependent, i.e., maximally 9 and 5 mM sulfide at pH 7.2 and pH 6.5, respectively.This research was financially supported by a grant from the Technology Foundation STW (grant STW-WBC 5280), applied science division of NWO, the Netherlands, and Shell Global Solutions (Amsterdam, the Netherlands) and Paques B.V. (Balk, the Netherlands).Peer reviewe

FEATURES OF VASCULAR ENDOTHELIAL FUNCTIONING IN HEALTHY PERSONS IN DIFFERENT PERIODS OF SOLAR ACTIVITY

Functional properties of vascular endothe­lium in healthy persons had been studied by probe with in­duced ischemia depending on a solar activity (SA) period during the 23rd solar cycle. The blood viscosity had been es­timated before and after occlusion test. The nascency of an­giospasm in a low SA is accompanied by adaptive increase of a blood flow property, and in a high SA this mechanism is been losing. So, in healthy persons the period of SA deter­mines functional properties of vascular endothelium. 

Epidemiology of the syncope in children and adolescents in elite sport (EPISODE-S research)

Study purpose. To determine the prevalence of syncope among young elite athletes.Material and methods. In the course of the study “Epidemiology of the syncope in children and adolsceents in elite sport (EPISODE-S)” ** 1687 young elite athletes 16.3±1.5 years old, members of the Russian National teams from 52 sports discipline were surveyed. Control group was 1732 nonathletes the same age and gender.Results. 113 (6.7%) athletes had syncope (girls – 73.5%). More often, syncope registered in basketball (10.5%), judo (10.3%), rhythmic gymnastics (9.1%), figure skating (8.0%), volleyball (7.4%). According to the results of questioning in schools, syncope was noted in 4.2% of students. In all cases among athletes syncope had neurally mediated (reflex) nature by the results of the additional examination. Four out of 1687 athletes (0.24%) had the long QT syndrome, but they haven’t got syncope.Conclusions. 1. In the young elite athletes prevalence of syncope is 6.7% (4.2% for non-athletes) and significantly more common in girls (11.6% vs. 3.4%). More often, syncope occurs in sports where tall athletes are selected (basketball, volleyball) or strict weight control is required (rhythmic gymnastics, figure skating, judo). The majority of the young athletes has benign neurally mediated nature of syncope and doesn’t need restrict for the sport, but the first of all they require the exclusion of diseases with a high risk of sudden cardiac death.** Epidemiology of the syncope in children and adolsceents in elite sport (EPISODE-S