A novel transcriptome subtraction method for the detection of differentially expressed genes in highly complex eukaryotes

We have designed a novel transcriptome subtraction method for the genome-scale analysis of differential gene expression in highly complex eukaryotes, in which suppression subtractive hybridization (SSH) is performed first to enrich the target and, after exchange of adapters, negative subtraction chain (NSC) is then used to eliminate the remaining background. NSC evolved from differential subtraction chain (DSC). We designed novel adapters which make the subtraction system more robust. SSH and NSC were then combined to successfully detect differentially expressed genes in Solanum. The combined technique improves qualitatively upon SSH, the only commercially available transcriptome subtraction system, by detecting target genes in the middle abundance class, to which most differentially expressed genes in highly complex eukaryotes are expected to belong. The main advantage of the combined technique with SSH/NSC is its ability to isolate differentially expressed genes quickly and cost-efficiently from non-standard models, for those microarrays are unavailable

High spatial resolution and high contrast optical speckle imaging with FASTCAM at the ORM

In this paper, we present an original observational approach, which combines, for the first time, traditional speckle imaging with image post-processing to obtain in the optical domain diffraction-limited images with high contrast (1e-5) within 0.5 to 2 arcseconds around a bright star. The post-processing step is based on wavelet filtering an has analogy with edge enhancement and high-pass filtering. Our I-band on-sky results with the 2.5-m Nordic Telescope (NOT) and the lucky imaging instrument FASTCAM show that we are able to detect L-type brown dwarf companions around a solar-type star with a contrast DI~12 at 2" and with no use of any coronographic capability, which greatly simplifies the instrumental and hardware approach. This object has been detected from the ground in J and H bands so far only with AO-assisted 8-10 m class telescopes (Gemini, Keck), although more recently detected with small-class telescopes in the K band. Discussing the advantage and disadvantage of the optical regime for the detection of faint intrinsic fluxes close to bright stars, we develop some perspectives for other fields, including the study of dense cores in globular clusters. To the best of our knowledge this is the first time that high contrast considerations are included in optical speckle imaging approach.Comment: Proceedings of SPIE conference - Ground-based and Airborne Instrumentation for Astronomy III (Conference 7735), San Diego 201

Declarative Choreographies and Liveness

Part 1: Full PapersInternational audienceWe provide the first formal model for declarative choreographies, which is able to express general omega-regular liveness properties. We use the Dynamic Condition Response (DCR) graphs notation for both choreographies and end-points. We define end-point projection as a restriction of DCR graphs and derive the condition for end-point projectability from the causal relationships of the graph. We illustrate the results with a running example of a Buyer-Seller-Shipper protocol. All the examples are available for simulation in the online DCR workbench at http://dcr.tools/forte19

An Executive Appraisal of Courses Which Best Prepare One for General Management

This ongoing study summarizes 1980-81 data from 1158 newly promoted executives in the United States who answered this question: "Assuming the study of business administration best prepares a young person for a career in general management, how important are the following courses as a part of that preparation?" Business Communication-oral and written- was the course selected as Very Important more often than any of thirteen courses.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/68655/2/10.1177_002194368201900102.pd

Uncovering the (un-)occupied electronic structure of a buried hybrid interface

The energy level alignment at organic/inorganic (o/i) semiconductor interfaces is crucial for any light-emitting or -harvesting functionality. Essential is the access to both occupied and unoccupied electronic states directly at the interface, which is often deeply buried underneath thick organic films and challenging to characterize. We use several complementary experimental techniques to determine the electronic structure of p-quinquephenyl pyridine (5P-Py) adsorbed on ZnO(10-10). The parent anchoring group, pyridine, significantly lowers the work function by up to 2.9 eV and causes an occupied in-gap state (IGS) directly below the Fermi level $E_\text{F}$. Adsorption of upright-standing 5P-Py also leads to a strong work function reduction of up to 2.1 eV and to a similar IGS. The latter is then used as an initial state for the transient population of three normally unoccupied molecular levels through optical excitation and, due to its localization right at the o/i interface, provides interfacial sensitivity, even for thick 5P-Py films. We observe two final states above the vacuum level and one bound state at around 2 eV above $E_\text{F}$, which we attribute to the 5P-Py LUMO. By the separate study of anchoring group and organic dye combined with the exploitation of the occupied IGS for selective interfacial photoexcitation this work provides a new pathway for characterizing the electronic structure at buried o/i interfaces

Cosmological surveys with the Australian Square Kilometre Array Pathfinder

This is a design study into the capabilities of the Australian Square Kilometre Array Pathfinder in performing a full-sky low redshift neutral hydrogen survey, termed WALLABY, and the potential cosmological constraints one can attain from measurement of the galaxy power spectrum. We find that the full sky survey will likely attain 0.6 million redshifts which, when combined with expected Planck CMB data, will constrain the Dark Energy equation of state to 20%, representing a coming of age for radio observations in creating cosmological constraints.Comment: 10 pages, 6 figures, accepted in PASA, updated to match published versio

Feature Lines for Illustrating Medical Surface Models: Mathematical Background and Survey

This paper provides a tutorial and survey for a specific kind of illustrative visualization technique: feature lines. We examine different feature line methods. For this, we provide the differential geometry behind these concepts and adapt this mathematical field to the discrete differential geometry. All discrete differential geometry terms are explained for triangulated surface meshes. These utilities serve as basis for the feature line methods. We provide the reader with all knowledge to re-implement every feature line method. Furthermore, we summarize the methods and suggest a guideline for which kind of surface which feature line algorithm is best suited. Our work is motivated by, but not restricted to, medical and biological surface models.Comment: 33 page