Novo neonicotinóide associado a regulador de crescimento de insetos, no controle da mosca-branca e do pulgão na cultura do algodão.

Resumo: O objetivo deste trabalho foi avaliar o desempenho da nova molécula química dinotefuram, pertencente ao grupo dos neonicotinóides, associada a um análogo do hormônio juvenil (piriproxifem), em aplicação foliar, no controle da mosca-branca B. tabaci Biótipo B e do pulgão A. gossypii, em algodoeiro. O delineamento foi em blocos ao acaso com quatro repetições. Os tratamentos foram: testemunha, dinotefuram + piriproxifem a 25 + 12,5; 50 + 12,5; 75 + 12,5; 25 + 25; 50 + 25; 75 + 25 e dinotefuram a 75 g i.a./ha. Cada parcela foi de 48m2. Para aplicação foliar, utilizou-se um pulverizador costal equipado com barra contendo seis pontas cônicas (TXVK-8), propelido por CO2 e volume de 200 L/ha. Foram realizadas avaliações de eficiência dos tratamentos aos 0, 5, 10, 15 e 20 dias após a aplicação, contando-se o número de ninfas de mosca-branca e o número de pulgões em 10 folhas por parcela. Pela análise dos resultados, concluiu-se que o inseticida dinotefuram associado ao piriproxifem, foi eficiente no controle de ninfas de mosca-branca e do pulgão, igualando-se aos padrões já utilizados

Potential application for antimicrobial and antileukemic therapy of a flavonoid-rich fraction of Camellia sinensis

The antimicrobial and antileukemic effect of a purified fraction of flavonoids from the leaves of Camellia sinensis was evaluated. An extraction yield of 9.77 mg.g-1 total flavonoids was recovered through a pressurized liquid extraction associated with solid-phase extraction. This fraction was tested against pathogenic microorganisms (Staphylococcus, Salmonella, and Enterococcus), considering the minimum inhibitory concentration. In addition, the human monocyte cell line THP-1, derived from a patient with acute monocytic leukemia, was used for the antitumor assay. The results show that the flavonoid-rich fraction obtained by coupling a Pressurized Liquids Extraction in-line with a Solid Phase Extraction (PLE-SPE) has a high antimicrobial effect and resulted in cell cycle blockage G0 / G1, increased DNA fragmentation, and altered leukemic cell morphology. These results suggest that a flavonoid-rich fraction obtained from Camellia sinensis can be applied as potential adjuvants in chemotherapy treatment to mitigate the side effects caused by chemotherapy or even as a supplement to cancer therapy9 página

Previsão probabilística de enchentes para uma pequena bacia hidrográfica do Pantanal.

O monitoramento para a previsão de cheias de pequenas bacias hidrográficas é de grande importância tendo em vista a relação dos recursos hídricos com a sociedade, pois pode garantir o uso sustentável às comunidades urbanas de cidades lindeiras à bacia. O rio Aquidauana está inserido na planície Pantaneira sendo considerado vulnerável à inundações, no entanto, falta de um sistema eficiente para previsões de cheias e inundações. Assim, este estudo propõe um sistema de previsão probalística de enchentes para a bacia do Rio Aquidauana. Para tanto foram utilizadas as redes neurais artificiais (RNAs) do tipo MultiLayer Perceptron (treinamento backpropagation) com parâmetros otimizados pelos Algoritmos Genéticos. A RNA foi treinada e avaliada com base em dados de chuva acumulada (mm) e nível de rio (cm) à montante entre os anos de 1995 a 2014. A previsão realizada foi de 1 a 5 dias, tendo como melhor desempenho o modelo para 1 dia de previsão, com resultado de coeficiente de determinação e erro quadrático médio de 0,93 e 30 (cm), respectivamente

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Supercritical fluid and pressurized liquid extractions of phytonutrients from passion fruit by-products: economic evaluation of sequential multi-stage and single-stage processes

This work presents the economic evaluation of two processes to obtain: i) four extract fractions from passion fruit bagasse by sequential multi-stage process comprising three steps of supercritical fluid extraction (SFE) and one step of pressurized liquid extraction (PLE); ii) one extract from passion fruit rinds by single-stage PLE process. The economic simulation and sensitivity study were performed for plants containing two extraction vessels of 1, 5, 50 and 500 L. The scale-up led to a decrease in the cost of manufacturing (COM). COMs of extracts from sequential multi-stage and single-stage processes decreased from US$220.51/kg to US$ 26.33/kg and US$71.03/kg to US$ 11.96/kg, respectively, when the system capacity increased from 2 × 1 L to 2 × 500 L. Itemized costs related to materials, facilities, labor and utilities are also presented. Both processes are economically promising, especially when the extracts are produced in large scale and sold by the current market price1228898COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP2952/20112014/00372-8; 2013/02203-6; 2015/11932-

Multiple myeloma - oral radiological evidences

Objective: The aim of this article is to summarize the main radiological evidences induced by multiple myeloma at the jaws level. Materials and Methods: The analysis examines the main case reports and reviews on the matter. All relevant publications were searched through the PubMed search engine, using the following keywords: "Multiple Myeloma" and "Radiological evidences", "Maxilla", "Jaw", "TMJ". The purpose of our review is to highlight how oral radiological investigation is extremely useful in detecting any bone alterations associated with cases of multiple myeloma. In addition, we investigate the sites of the jaws where direct bone alteration is more frequently, and we perform a differential diagnosis with other focal alterations of the jaws or those that are the result of certain drug therapies (bisphosphonates). Our research stresses the concept that early diagnosis of multiple myeloma at the jaws level may prevent unnecessary and inadequate treatments. Results: From the evaluation of our research, it is evident that osteolytic lesions are one of the fundamental signs of multiple myeloma, especially in areas undergoing intense hematopoietic activity. Among the radiological techniques used for diagnosis, the most sensitive one appears to be the three-dimensional technique, such as CT or Cone Beam, whereas two-dimensional radiographs (orthopantomography) appear to be exhaustive only when the lesion is very extensive. Conclusions: It is evident that the dentist figure plays not only a supportive role, but also a fundamental part in the diagnostic process, as lesions that may anticipate a systemic manifestation are often encountered at the oral level.Sin financiaciónNo data 2020UE

Ultrasound-Assisted Extraction of Semi-Defatted Unripe Genipap (Genipa americana L.): Selective Conditions for the Recovery of Natural Colorants

Ultrasound-assisted extraction (UAE) of semi-defatted unripe genipap (SDG) using supercritical CO2 was performed to enhance the recovery of natural colorant iridoids genipin and geniposide. There are currently few natural sources of iridoids, and their application as colorants is scarce. The UAE resulted in extracts with blue and green colors using water and ethanol, respectively. The highest global yield and genipin content was recovered with water, and the geniposide was significantly recovered with ethanol. With water at 450 W, the UAE raised the maximum global yield (25.50 g/100 g raw material). At 150 W and 7 min, the maximum content of genipin (121.7 mg/g extract) and geniposide (312 mg/g extract) was recovered. The total phenolic content (TPC) and antioxidant capacity with the oxygen reactive antioxidant capacity (ORAC) assay were also high in aqueous extracts. Ethanolic extracts showed high ferric-reducing ability antioxidant potential (FRAP) values. UAE showed an efficient and fast method to obtain different extracts’ fractions from SDG, which have a wide spectrum of applications, especially as natural food colorants

Plaque composition in plexogenic and thromboembolic pulmonary hypertension: the critical role of thrombotic material in pultaceous core formation

Background: Patients with pulmonary hypertension develop intimal plaques in large pulmonary arteries. Objective: To test the hypothesis that the composition of such plaques differs depending on whether the aetiology of the disease is thromboembolic or hypertensive. Design: Chronic thromboembolic and plexogenic pulmonary hypertension (primary and secondary (Eisenmenger syndrome)) were investigated. These are spontaneous human models and were used to examine the independent role of thrombus and hypertension in plaque composition. Setting: A national tertiary referral centre for lung transplantation and pulmonary thromboendoarterectomy. Patients: Thirty nine patients with chronic thromboembolic pulmonary hypertension who had undergone thromboendoarterectomy (n = 32) or lung transplantation (n = 7), 28 with plexogenic diseases (nine primary and 19 Eisenmenger), and three with Eisenmenger syndrome complicated by thromboembolic events. Interventions: The lung and thromboendoarterectomy samples were sectioned, stained with Movat pentachrome, and immunostained with antibodies for fibrin, platelets, inflammatory cells, smooth muscle cells, and erythrocyte membrane glycophorin A. Main outcome measure: Composition of the plaques affecting large pulmonary arteries. Results: Two types of intimal lesion were distinguished in chronic thromboembolic pulmonary hypertension: fibrous plaques with angioneogenesis; and core-rich atherosclerotic plaques with pultaceous cores largely consisting of glycophorin immunoreactive material, with cholesterol clefts (61.5%), CD68 positive macrophages (84.6%), T lymphocytes (87%), and calcification (46.1%). The samples from the patients with Eisenmenger syndrome and thromboembolic complications had similar characteristics, whereas those from patients with uncomplicated primary pulmonary hypertension had core-free fibrous plaques, spotted with macrophages and T lymphocytes. Conclusions: Chronic thromboembolic pulmonary hypertension is associated with atherosclerotic plaques with glycophorin-rich pultaceous cores, and plexogenic pulmonary hypertension with fibrous plaques. Thromboembolic material thus plays a critical role in the formation of pultaceous cores, of which erythrocyte membrane derived glycophorin is a major component