1,343 research outputs found
The diagnosis of malaria infection using a solid-phase radioimmunoassay for the detection of malaria antigens: Application to the detection of Plasmodium berghei infection in mice
A method has been devised to show that malaria parasites can be detected serologically in infected blood with a high degree of sensitivity. Using a murine malaria model, parasites were demonstrated in a solid-phase radio-immunoassay which measured antibody-binding inhibition. Lysed red blood cells (r.b.c.) were incubated with labelled specific antibody and were then reacted in antigen-coated tubes. The degree of inhibition of antibody binding in the tubes correlated with the level of parasitaemia in the test blood. Using homologous antisera the test detected infection at a level of 1 parasite/million r.b.c. The specificity of the method was shown by comparison of antibody-binding inhibition in normal and infected r.b.c. and in r.b.c. from non-infected mice with induced reticulocytosis. The sensitivity was shown in vitro in tests of serially diluted blood of high parasitaemia and in vivo for the detection of early infection. The presence of antibody in the test blood did not significantly affect the sensitivity of parasite detectio
Quasi-monoenergetic femtosecond photon sources from Thomson Scattering using laser plasma accelerators and plasma channels
Narrow bandwidth, high energy photon sources can be generated by Thomson
scattering of laser light from energetic electrons, and detailed control of the
interaction is needed to produce high quality sources. We present analytic
calculations of the energy-angular spectra and photon yield that parametrize
the influences of the electron and laser beam parameters to allow source
design. These calculations, combined with numerical simulations, are applied to
evaluate sources using conventional scattering in vacuum and methods for
improving the source via laser waveguides or plasma channels. We show that the
photon flux can be greatly increased by using a plasma channel to guide the
laser during the interaction. Conversely, we show that to produce a given
number of photons, the required laser energy can be reduced by an order of
magnitude through the use of a plasma channel. In addition, we show that a
plasma can be used as a compact beam dump, in which the electron beam is
decelerated in a short distance, thereby greatly reducing radiation shielding.
Realistic experimental errors such as transverse jitter are quantitatively
shown to be tolerable. Examples of designs for sources capable of performing
nuclear resonance fluorescence and photofission are provided
Effects of Hyperbolic Rotation in Minkowski Space on the Modeling of Plasma Accelerators in a Lorentz Boosted Frame
Laser driven plasma accelerators promise much shorter particle accelerators
but their development requires detailed simulations that challenge or exceed
current capabilities. We report the first direct simulations of stages up to 1
TeV from simulations using a Lorentz boosted calculation frame resulting in a
million times speedup, thanks to a frame boost as high as gamma=1300. Effects
of the hyperbolic rotation in Minkowski space resulting from the frame boost on
the laser propagation in the plasma is shown to be key in the mitigation of a
numerical instability that was limiting previous attempts
Multispectral images of peach related to firmness and maturity at harvest
wo multispectral maturity classifications for red soft-flesh peaches (‘Kingcrest’, ‘Rubyrich’ and ‘Richlady’ n = 260) are proposed and compared based on R (red) and R/IR (red divided by infrared) images obtained with a three CCD camera (800 nm, 675 nm and 450 nm). R/IR histograms were able to correct the effect of 3D shape on light reflectance and thus more Gaussian histograms were produced than R images. As fruits ripened, the R/IR histograms showed increasing levels of intensity. Reference measurements such as firmness and visible spectra also varied significantly as the fruit ripens, firmness decreased while reflectance at 680 nm increased (chlorophyll absorption peak)
The Effect of Dry Matter Content and Inoculation with Lactic Acid Bacteria on the Residual Water Soluble Carbohydrate Content of Silages Prepared from a High Sugar Grass Cultivar
The introduction of new perennial ryegrass cultivars bred for high water soluble carbohydrate (WSC) content has created opportunities for improving the quality of grass silage, by not only providing adequate WSC for a good fermentation, but also sufficient to leave a higher residual level of WSC in the mature silage. High WSC silages have the potential to provide readily available energy during the early stages of rumen fermentation to balance energy and nitrogen supply and optimise rumen microbial growth. (Merry et al. 2002). The aim was to examine the effect of wilting and silage inoculants on the residual WSC content of grass silage
Performing public credit at the eighteenth-century Bank of England
Much is known about the negotiation of personal credit relationships during the eighteenth century. It has been noted how direct contact and observation allowed individuals to assess the creditworthiness of those with whom they had financial connections and to whom they might lend money. Much less is known about one of the most important credit relationships of the long eighteenth century: that between the state and its creditors. This article shows that investors could experience the performance of public credit at the Bank of England. By 1760 the Bank was the manager of nearly three-quarters of the state's debt and housed the main secondary market in that debt. Thus, it provided a place for public creditors, both current and potential, to attend and scrutinize the performance of the state's promises. The article demonstrates how the Bank acted to embody public credit through its architecture, internal structures, and imagery and through the very visible actions of its clerks and the technologies that they used to record ownership and transfer of the national debt. The Bank of England, by those means, allowed creditors to interrogate the financial stability and reputation of the state in the same ways that they could interrogate the integrity of a private debtor.Peer reviewedFinal Accepted Versio
Flap reconstruction of the hypopharynx: a defect orientated approach
The present retrospective analysis evaluated the outcomes of different flap reconstructions for several hypopharyngeal defects in 136 patients who underwent hypopharyngeal reconstruction with a free or pedicled flap after excision of pharyngeal or laryngeal carcinoma.Functional and oncological outcome were the main measures. Nine patients had a type I-a hypopharyngeal defect (partial with larynx preserved), 33 type I-b (partial without larynx preserved), 85 type II (circumferential), 5 type III (extensive superior) and 4 vertical hemipharyngolaryngectomy. The flaps used to reconstruct these defects were pectoralis major (n = 34), free radial forearm (n = 25), jejunum (n = 72), pedicled latissimus dorsi (n = 2), sternocleidomastoid (n = 1), lateral thigh (n = 1) and deltopectoral (n = 1). Twelve defects (9%) needed a secondary flap reconstruction. Surgical and medical complications were seen in 29% and 8% of patients, respectively; 18% of patients developed a fistula. No difference in complication rate or admission days was found for pre-operative versus no previous radiotherapy, type of defect or free versus pedicled flap. After 12 months follow-up, 38% of patients had a tracheo-oesophageal voice prosthesis, in 82% a fully oral diet was obtained and the average body weight gain was 0.9 kg. Five-year overall and disease-specific survival rates were 35% and 49%, respectively, while local and regional control rates were 65% and 91%, respectively. Considering these results, a defect orientated approach may be helpful for deciding which flap should be used for reconstruction of the hypopharynx. An algorithm is proposed with similar functional and oncological outcomes for the different groups. The choice of flap should be based on expected morbidity and functional outcome
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