53 research outputs found
Cardiac and Respiratory Patterns Synchronize between Persons during Choir Singing
Dyadic and collective activities requiring temporally coordinated action are likely to be associated with cardiac and respiratory patterns that synchronize within and between people. However, the extent and functional significance of cardiac and respiratory between-person couplings have not been investigated thus far. Here, we report interpersonal oscillatory couplings among eleven singers and one conductor engaged in choir singing. We find that: (a) phase synchronization both in respiration and heart rate variability increase significantly during singing relative to a rest condition; (b) phase synchronization is higher when singing in unison than when singing pieces with multiple voice parts; (c) directed coupling measures are consistent with the presence of causal effects of the conductor on the singers at high modulation frequencies; (d) the different voices of the choir are reflected in network analyses of cardiac and respiratory activity based on graph theory. Our results suggest that oscillatory coupling of cardiac and respiratory patterns provide a physiological basis for interpersonal action coordination
Identification and Characterisation of a Novel Acylpeptide Hydrolase from Sulfolobus Solfataricus: Structural and Functional Insights
A novel acylpeptide hydrolase, named APEH-3Ss, was isolated from the hypertermophilic archaeon Sulfolobus solfataricus. APEH is a member of the prolyl oligopeptidase family which catalyzes the removal of acetylated amino acid residues from the N terminus of oligopeptides. The purified enzyme shows a homotrimeric structure, unique among the associate partners of the APEH cluster and, in contrast to the archaeal APEHs which show both exo/endo peptidase activities, it appears to be a “true” aminopeptidase as exemplified by its mammalian counterparts, with which it shares a similar substrate specificity. Furthermore, a comparative study on the regulation of apeh gene expression, revealed a significant but divergent alteration in the expression pattern of apeh-3Ss and apehSs (the gene encoding the previously identified APEHSs from S. solfataricus), which is induced in response to various stressful growth conditions. Hence, both APEH enzymes can be defined as stress-regulated proteins which play a complementary role in enabling the survival of S. solfataricus cells under different conditions. These results provide new structural and functional insights into S. solfataricus APEH, offering a possible explanation for the multiplicity of this enzyme in Archaea
The serine protease domain of MASP-3: enzymatic properties and crystal structure in complex with ecotin.
International audienceMannan-binding lectin (MBL), ficolins and collectin-11 are known to associate with three homologous modular proteases, the MBL-Associated Serine Proteases (MASPs). The crystal structures of the catalytic domains of MASP-1 and MASP-2 have been solved, but the structure of the corresponding domain of MASP-3 remains unknown. A link between mutations in the MASP1/3 gene and the rare autosomal recessive 3MC (Mingarelli, Malpuech, Michels and Carnevale,) syndrome, characterized by various developmental disorders, was discovered recently, revealing an unexpected important role of MASP-3 in early developmental processes. To gain a first insight into the enzymatic and structural properties of MASP-3, a recombinant form of its serine protease (SP) domain was produced and characterized. The amidolytic activity of this domain on fluorescent peptidyl-aminomethylcoumarin substrates was shown to be considerably lower than that of other members of the C1r/C1s/MASP family. The E. coli protease inhibitor ecotin bound to the SP domains of MASP-3 and MASP-2, whereas no significant interaction was detected with MASP-1, C1r and C1s. A tetrameric complex comprising an ecotin dimer and two MASP-3 SP domains was isolated and its crystal structure was solved and refined to 3.2 Å. Analysis of the ecotin/MASP-3 interfaces allows a better understanding of the differential reactivity of the C1r/C1s/MASP protease family members towards ecotin, and comparison of the MASP-3 SP domain structure with those of other trypsin-like proteases yields novel hypotheses accounting for its zymogen-like properties in vitro
Mechanisms of Intramolecular Communication in a Hyperthermophilic Acylaminoacyl Peptidase: A Molecular Dynamics Investigation
Protein dynamics and the underlying networks of intramolecular interactions and communicating residues within the three-dimensional (3D) structure are known to influence protein function and stability, as well as to modulate conformational changes and allostery. Acylaminoacyl peptidase (AAP) subfamily of enzymes belongs to a unique class of serine proteases, the prolyl oligopeptidase (POP) family, which has not been thoroughly investigated yet. POPs have a characteristic multidomain three-dimensional architecture with the active site at the interface of the C-terminal catalytic domain and a β-propeller domain, whose N-terminal region acts as a bridge to the hydrolase domain. In the present contribution, protein dynamics signatures of a hyperthermophilic acylaminoacyl peptidase (AAP) of the prolyl oligopeptidase (POP) family, as well as of a deletion variant and alanine mutants (I12A, V13A, V16A, L19A, I20A) are reported. In particular, we aimed at identifying crucial residues for long range communications to the catalytic site or promoting the conformational changes to switch from closed to open ApAAP conformations. Our investigation shows that the N-terminal α1-helix mediates structural intramolecular communication to the catalytic site, concurring to the maintenance of a proper functional architecture of the catalytic triad. Main determinants of the effects induced by α1-helix are a subset of hydrophobic residues (V16, L19 and I20). Moreover, a subset of residues characterized by relevant interaction networks or coupled motions have been identified, which are likely to modulate the conformational properties at the interdomain interface
Mozart K.448 listening decreased seizure recurrence and epileptiform discharges in children with first unprovoked seizures: a randomized controlled study
Molecular mechanisms of pH-tunable stability and surface coverage of polypeptide films
Funding Information: This work was supported by the Academy of Finland through its Centres of Excellence Programme (2022–2029, LIBER) under project no. 346111 (M.S.) , Business Finland Co-Innovation grant No. 3767/31/ 2019 (M.S.) , the National Science Centre, Poland (grant no. 2018/31/D/ ST5/01866 ) (P.B. and M.M.), and the U.S. National Science Foundation , grant No. 1905732 (J.L.). We are grateful for the support by FinnCERES Materials Bioeconomy Ecosystem. Computational resources by CSC IT Centre for Finland, PLGrid Infrastructure (Poland), and RAMI – RawMatters Finland Infrastructure are also gratefully acknowledged. Publisher Copyright: © 2023 The AuthorsStreaming potential and quartz crystal microbalance measurements, combined with all-atom molecular dynamics simulations, were used to study the pH dependency of the adsorption of two basic homopolypeptides, poly-L-lysine (PLL) and poly-L-arginine (PARG), on α-quartz surface. We report that the observed adsorption behavior rises from an interplay of i) the change in the number of possible peptide-surface ion pairs between the charged moieties and ii) repulsive electrostatic interactions between the polypeptide molecules. For low pH values, polypeptide adsorption was strongest and stable monolayers were formed. However, electrostatic repulsion between the polypeptides led to a relatively low maximum surface coverage. On the other hand, higher pH led to more weakly bound, but significantly denser, peptide films with limited stability. Simulations indicate that electrostatic interactions are the main driving force for adsorption, while hydrogen bonding and non-specific interactions also contribute. Additionally, the important role of the counterions of the negatively charged quartz surface that form a positively charged ion adlayer is highlighted. Ion release of the condensed sodium ions at the charged surface occurs via displacement by polypeptide adsorption. The mechanisms revealed by this work provide systematic guidelines to engineering active surfaces of charged peptides with controlled surface coverage and reversible binding.Peer reviewe
Can flow alleviate anxiety? The roles of academic self-efficacy and self-esteem in building psychological sustainability and resilience.
A growing number of studies suggest that flow experience is associated with life satisfaction,
eudaimonic well-being, and the perceived strength of one’s social and place identity. However, little
research has placed emphasis on flow and its relations with negative experiences such as anxiety.
The current study investigated the relations between flow and anxiety by considering the roles of
self-esteem and academic self-ecacy. The study sample included 590 Chinese university students,
who were asked to complete a self-report questionnaire on flow, anxiety, self-esteem, and academic
self-ecacy. Data were analyzed using structural equation modeling (SEM) with AMOS software, in
which both factorial analysis and path analysis were performed. Results revealed that the experience
of flow negatively predicted anxiety, and both self-esteem and academic self-ecacy fully mediated
the path between flow and anxiety. Specifically, self-esteem played a crucial and complete mediating
role in this relationship, while academic self-ecacy mediated the path between self-esteem and
anxiety. Our findings enrich the literature on flow experience and help with identifying practical
considerations for buering anxiety and more broadly with fostering strategies for promoting
psychological sustainability and resilience
Dissipative particle dynamics simulations of H-shaped diblock copolymer self-assembly in solvent
We examine the self-assembly of H-shaped block-copolymers as the function of the middle block to branch length
ratio and interaction between the middle and branch blocks differing in their solvophobicity. The work shows
that the examined H-shaped polymers readily transition from uniform mixing of the polymer species to domain
formation and a variety of advanced assembly configurations including vesicles, onion-like, and multi compartment aggregates. We identify the polymer conformational and packing changes involved to extract
governing interactions and molecule features giving rise to the different assembly structures. The findings are
discussed in terms of the H-shaped polymer architecture and polymer assemblies. We conclude that the assembly
structure is governed by the molecular level local curvature induced by the varying conformations of the
polymers. The findings highlight that for H-shaped polymers the degree of polymerization and polymer chem istries in terms of solvation and mixing characteristics of the blocks are keys to controlling the assembling
structures
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