11 research outputs found
Do We Need to Perform Cystoscopy on All Adults Attending Urology Centres as Outpatients?
Application of a Library of Artificial Receptors Formed by the Self-Organization of N-Lipidated Peptides Immobilized on Cellulose in Studying the Effects of the Incorporation of a Fluorine Atom
On the microscopic mechanism behind the purely orientational disorder–disorder transition in the plastic phase of 1-chloroadamantane
Development and application of a 3D periodontal in vitro model for the evaluation of fibrillar biomaterials
Chasing Phosphohistidine, an Elusive Sibling in the Phosphoamino Acid Fa mily
This year (2012) marks the 50th anniversary of the discovery of protein histidine phosphorylation. Phosphorylation of histidine (pHis) is now widely recognized as being critical to signaling processes in prokaryotes and lower eukaryotes. However, the modification is also becoming more widely reported in mammalian cellular processes and implicated in certain human disease states such as cancer and inflammation. Nonetheless, much remains to be understood about the role and extent of the modification in mammalian cell biology. Studying the functional role of pHis in signaling, either in vitro or in vivo, has proven devilishly hard, largely due to the chemical instability of the modification. As a consequence, we are currently handicapped by a chronic lack of chemical and biochemical tools with which to study histidine phosphorylation. Here, we discuss the challenges associated with studying the chemical biology of pHis and review recent., progress that offers some hope that long-awaited biochemical reagents for studying this elusive posttranslational modification (PTM) might soon be availableclose353