Local anesthetic infiltration vs. nervous blocks in face’s skin lesions: what’s new

Skin tumors are the most common type of cancer. They are localized throughout the body, more frequently in those regions chronically exposed to sun, like face, scalp and neck, compromising aesthetic appearance. The optimization of day hospital surgical procedures is mandatory, to avoid erroneous indications, insufficient intra operative comfort and prolonged recovery. New guidelines should be discussed and shared. Patients were divided in two groups: i. Group A of 50 patients, 21 male and 29 female, age 65 ±9, ASA I – III (10/19/21), weight 68±11 kg, height 160±8, with anesthetic Local Infiltration (LI); ii. Group B of 50 patients, 16 male, 34 female, age 68 ±10, ASA I – III (9/22/19), weight 64 ± 9 kg, height 158 ±11, with nerve block (NB). The purpose of our study is to evaluate the analgesia level, compliance and complication rate after LI or selective NB with alkalinised mepivacaine cloridrate 2%, Guardant®. Demographic data, ASA physical stauts, size of lesions, surgery, anesthesia durations and volume of LA injected were analyzed. Fisher’s exact test and Student’s t test were used; P ≤ 0.05 was considered statistically significant. No differences in age (65 ± 9 vs 68 ± 10 years), weight (68 ± 11 vs 64 ± 9), height (160 ± 8 vs 158 ± 11 cm), size of lesion (23 ± 11 vs 25 ± 14 mm), duration of surgery (47 ± 18 vs 51 ± 23 minutes) were detected in two groups (p > 0.05). Both anesthetic techniques ensured good analgesia, but only nerve’s blocks were be able to determine satisfactory intra operative patient’s comfort, a bloodless wound and weak risk for nervous lesions and toxic reaction to local anesthetic

Evidence for a Sympatric Origin of Ribolla gialla, Gouais Blanc and Schiava cultivars (V. vinifera L.)

Ribolla gialla is an autochthonous grape variety cultivated in Friuli Venezia Giulia (Italy) and in Slovenia, and probably originated in Eastern Central Europe. Just like Ribolla gialla, the Gouais blanc and Schiava cultivars also appear to have originated in the same geographical area. To verify this hypothesis, a pool of varieties probably sharing the same historical and geographical origin were taken into account. The fingerprinting, by 35 SSR loci, of Gouais blanc, Ribolla gialla, Schiava grossa and Schiava lombarda (synonyms of Schiava bresciana), is presented and the correlation among historical, geographical and genetic information of these cultivars was investigated. Gouais blanc and Traminer, already suggested as key varieties in the development of European grape diversity and as parents for some French varieties, interestingly enough seems to be linked to Ribolla gialla. The putative parentage was verified using 58 microsatellite markers. The genetic results suggest a common geographical origin for Gouais blanc, Ribolla gialla and the Schiava group: these cultivars appear to be related through sympatric origin. The hypothesis of Ribolla gialla as a progeny of Gouais blanc and Traminer was ruled out. The data proved a second-degree relationship between Gouais blanc and Ribolla gialla and a third or more distant degree of relationships between Ribolla gialla and Traminer

Evidence for a Sympatric Origin of Ribolla gialla, Gouais Blanc and Schiava cultivars (V. vinifera L.)

Ribolla gialla is an autochthonous grape variety cultivated in Friuli Venezia Giulia (Italy) and in Slovenia,and probably originated in Eastern Central Europe. Just like Ribolla gialla, the Gouais blanc and Schiavacultivars also appear to have originated in the same geographical area. To verify this hypothesis, a poolof varieties probably sharing the same historical and geographical origin were taken into account. Thefingerprinting, by 35 SSR loci, of Gouais blanc, Ribolla gialla, Schiava grossa and Schiava lombarda(synonyms of Schiava bresciana), is presented and the correlation among historical, geographical andgenetic information of these cultivars was investigated. Gouais blanc and Traminer, already suggested askey varieties in the development of European grape diversity and as parents for some French varieties,interestingly enough seems to be linked to Ribolla gialla. The putative parentage was verified using 58microsatellite markers. The genetic results suggest a common geographical origin for Gouais blanc,Ribolla gialla and the Schiava group: these cultivars appear to be related through sympatric origin. Thehypothesis of Ribolla gialla as a progeny of Gouais blanc and Traminer was ruled out. The data proved asecond-degree relationship between Gouais blanc and Ribolla gialla and a third or more distant degree ofrelationships between Ribolla gialla and Traminer

Effects of genotype and environmental conditions on grapevine (Vitis vinifera L.) shoot morphology

Grapevine shoot morphology is widely studied for both ampelography and growing adaptation to environmental stresses. However, few is known concerning the relative contribution and interactions of the genotype and of the growing conditions to the vegetative growth. In this work, seven grapevine cultivars were studied in three geographically distant ampelographic collections to maximize the genotype and environment differences among samples. Phytomers were studied concerning the leaf area and the stem and petiole diameters and lengths. These measurements allowed the calculation of derivative parameters to describe the proportions among elements. Despite most of the studied parameters significantly discriminated both factors (cultivar and growing conditions), it was possible to identify, for each one of them, the most promising parameters based on their relative variance explanation. In fact, a negative correlation was observed between the roles of genotype and environment among the studied parameters. The low interaction effect suggested a stability in the plant behaviors, confirming the possibility to use vegetative descriptions for both cultivar discrimination and growing conditions. Future studies will be performed to develop specific indexes based on the phenotypical variability of shoot morphology described here

Water deficit effects on grapevine woody tissue pigmentations

Water availability is an important environmental factor in viticulture. In a climate change context, vineyard management should be adapted to the new conditions. Drought-resistant rootstocks need to be selected. In this paper, reflectance spectroscopy is proposed as a new method to characterize the water stress effects on woody section pigmentations. Cabernet Sauvignon grafted on 4 different rootstocks (140Ru, 420A, M2 and M3) represented the plant material. Greenhouse controlled conditions allowed the comparison of well-watered (WW) and water-stressed (WS) plants. The physiological responses were characterized concerning daily water consumption, stem water potential, gas exchange, and plant growth. The water use efficiency was calculated and discussed as well. Spectroscopy analyses of woody sections indicated a major absorption band probably related to phenolic derivatives. Water stress produced characteristic spectrum modifications both in the Cabernet Sauvignon stem and in the rootstock xylem. These preliminary results encourage further studies addressed at the evaluation of drought-resistant genotypes, to distinguish their stress responses and to characterize the compositional aspects linked to drought tolerance

Application of visible/near infrared spectroscopy to assess the grape infection at the winery

The aim of this work was to investigate the applicability of vis/NIR spectroscopy for rapid grape infection assessment in a view of a grape classification directly at the check point station entering the winery. The experimentation was conducted on white and red wine varieties, using grape bunches naturally infected with Botrytis cinerea, powdery mildew and sour rot, the major grape diseases. The research applied a compact vis/NIR device (400-1600 nm) for analysis of flows and/or non-homogeneous product. The system is capable to perform measurements in reflection at a variable distance between sensor and sample of 80-600 mm. Spectral measurements were carried out on healthy and diseased bunches for a total of 2559 spectral acquisitions; Qualitative (spectral analysis and Principal Component Analysis, PCA) and quantitative (Partial Least Squares \u2013 Discriminant Analysis, PLS-DA) analyses were applied on grape and must spectra in order to test the performance of vis/NIR device to classify healthy and infected samples. The results obtained from PLS-DA models, in validation, gave a positive predictive value (PPV) of classification between 89.8% and 94.0% for grape. The results demonstrated that optical devices are capable to provide useful information for a better management of the vinification process

Assessing the Effectiveness of Variable-Rate Drip Irrigation on Water Use Efficiency in a Vineyard in Northern Italy

Although many studies in the literature illustrate the numerous devices and methodologies nowadays existing for assessing the spatial variability within agricultural fields, and indicate the potential for variable-rate irrigation (VRI) in vineyards, only very few works deal with the implementation of VRI systems to manage such heterogeneity, and these studies are usually conducted in experimental fields for research aims. In this study, a VR drip irrigation system was designed for a 1-ha productive vineyard in Northern Italy and managed during the agricultural season 2018, to demonstrate feasibility and effectiveness of a water supply differentiated according to the spatial variability detected in field. Electrical resistivity maps obtained by means of an electro-magnetic induction sensor were used to detect four homogeneous zones with similar soil properties. In each zone, a soil profile was opened, and soil samples were taken and analyzed in laboratory. Two irrigation management zones (MZs) were identified by grouping homogeneous zones on the basis of their hydrological properties, and an irrigation prescription map was built consistently with the total available water (TAW) content in the root zone of the two MZs. The designed drip irrigation system consisted of three independent sectors: the first two supplied water to the two MZs, while the third sector (reference sector) was managed following the farmer\u2019s habits. During the season, irrigation in the first two sectors was fine-tuned using information provided by soil moisture probes installed in each sector. Results showed a reduction of water use by 18% compared to the \u2018reference\u2019 sector without losses in yield and product quality, and a grape\u2019s maturation more homogeneous in time

SNP genotyping elucidates the genetic diversity of Magna Graecia grapevine germplasm and its historical origin and dissemination

Background: Magna Graecia is the ancient name for the modern geopolitical region of South Italy extensively populated by Greek colonizers, shown by archeological and historical evidence to be the oldest wine growing region of Italy, crucial for the spread of specialized viticulture around Mediterranean shores. Here, the genetic diversity of Magna Graecia grape germplasm was assessed and its role in grapevine propagation around the Mediterranean basin was underlined. Results: A large collection of grapevines from Magna Graecia was compared with germplasm from Georgia to the Iberian Peninsula using the 18 K SNP array. A high level of genetic diversity of the analyzed germplasm was determined; clustering, structure analysis and DAPC (Discriminant Analysis of Principal Components) highlighted the genetic relationships among genotypes from South Italy and the Eastern Mediterranean (Greece). Gene flow from east (Georgia) to west (Iberian Peninsula) was identified throughout the large number of detected admixed samples. Pedigree analysis showed a complex and well-structured network of first degree relationships, where the cultivars from Magna Graecia were mainly involved. Conclusions: This study provided evidence that Magna Graecia germplasm was shaped by historical events that occurred in the area due to the robust link between South Italian and Greek genotypes, as well as, by the availability of different thermal resources for cultivars growing in such different winegrowing areas. The uniqueness of this ampelographic platform was mainly an outcome of complex natural or human-driven crosses involving elite cultivars

Drought tolerance in grapevine rootstocks: an association genetics approach

Water deficit is widely recognized as one of the major constraint in the Mediterranean and semi-arid regions where a large part of the world's premium wines are produced. Since the introduction of new varieties in the wine industry is not so straightforward, breeding grapevine rootstocks for tolerance to drought is becoming a key strategy for the future. A Genome Wide Association Study (GWAS) and a Candidate Gene (CG) approach were used to investigate the genetic basis of drought response mechanisms in an ad hoc core-collection consisting of 96 different genotypes of Vitis spp. and hybrids, selected to ensure the maximum genetic variability of a larger population of commercial, germplasm and new bred rootstocks. The physiological and growth responses to water deficit of more than 500 one-year old potted cuttings were evaluated over 30 days in semi-controlled conditions. For each genotype, three well-watered control plants were mantained at 90% of the Field Capacity (FC) determined by gravimetric method and three plants were subjected to water stress. After 7 days, water deficit was gradually established to reach first a moderate stable water deficit (50% FC for 7 days) and then a more severe and stable water deficit (30% FC for 7 days). Finally, stressed plants were fully irrigated to evaluate the level of plant recovery. Stomatal conductance was correlated with plant growth evaluation (leaves and shoots biometric measures) to define different response classes and thermal infrared imaging was implemented for the first time as a valuable remote sensing tool for high-throughput phenotyping in a GWAS experiment. Acknowledgements: Italian "Progetto AGER, bando Viticoltura da Vino" (SERRES, 2010-2105

SNP genotyping elucidates the genetic diversity of Magna Graecia grapevine germplasm and its historical origin and dissemination

BACKGROUND: Magna Graecia is the ancient name for the modern geopolitical region of South Italy extensively populated by Greek colonizers, shown by archeological and historical evidence to be the oldest wine growing region of Italy, crucial for the spread of specialized viticulture around Mediterranean shores. Here, the genetic diversity of Magna Graecia grape germplasm was assessed and its role in grapevine propagation around the Mediterranean basin was underlined. RESULTS: A large collection of grapevines from Magna Graecia was compared with germplasm from Georgia to the Iberian Peninsula using the 18\u2009K SNP array. A high level of genetic diversity of the analyzed germplasm was determined; clustering, structure analysis and DAPC (Discriminant Analysis of Principal Components) highlighted the genetic relationships among genotypes from South Italy and the Eastern Mediterranean (Greece). Gene flow from east (Georgia) to west (Iberian Peninsula) was identified throughout the large number of detected admixed samples. Pedigree analysis showed a complex and well-structured network of first degree relationships, where the cultivars from Magna Graecia were mainly involved. CONCLUSIONS: This study provided evidence that Magna Graecia germplasm was shaped by historical events that occurred in the area due to the robust link between South Italian and Greek genotypes, as well as, by the availability of different thermal resources for cultivars growing in such different winegrowing areas. The uniqueness of this ampelographic platform was mainly an outcome of complex natural or human-driven crosses involving elite cultivars