Characterization of Adherent Bacteroidales from Intestinal Biopsies of Children and Young Adults with Inflammatory Bowel Disease

There is extensive evidence implicating the intestinal microbiota in inflammatory bowel disease [IBD], but no microbial agent has been identified as a sole causative agent. Bacteroidales are numerically dominant intestinal organisms that associate with the mucosal surface and have properties that both positively and negatively affect the host. To determine precise numbers and species of Bacteroidales adherent to the mucosal surface in IBD patients, we performed a comprehensive culture based analysis of intestinal biopsies from pediatric Crohn's disease [CD], ulcerative colitis [UC], and control subjects. We obtained biopsies from 94 patients and used multiplex PCR or 16S rDNA sequencing of Bacteroidales isolates for species identification. Eighteen different Bacteroidales species were identified in the study group, with up to ten different species per biopsy, a number higher than demonstrated using 16S rRNA gene sequencing methods. Species diversity was decreased in IBD compared to controls and with increasingly inflamed tissue. There were significant differences in predominant Bacteroidales species between biopsies from the three groups and from inflamed and uninflamed sites. Parabacteroides distasonis significantly decreased in inflamed tissue. All 373 Bacteroidales isolates collected in this study grew with mucin as the only utilizable carbon source suggesting this is a non-pathogenic feature of this bacterial order. Bacteroides fragilis isolates with the enterotoxin gene [bft], previously associated with flares of colitis, were not found more often at inflamed colonic sites or within IBD subjects. B. fragilis isolates with the ability to synthesize the immunomodulatory polysaccharide A [PSA], previously shown to be protective in murine models of colitis, were not detected more often from healthy versus inflamed tissue

Spin-polarized multiexcitons in quantum dots in the presence of spin-orbit interaction

An efficient electron spin-relaxation mechanism has been observed in InAs quantum dots (QDs) that manifests itself as a sharp drop in the circular polarization of the light emitted by Fe spin-light emitting diodes, which incorporate a single layer of InGaAs QDs, for a narrow range of magnetic fields around 5 T. The underlying mechanism occurs when the QDs are occupied by three-electron-hole pairs forming a tri-exciton (3X) and is a two-step process. The first step involves the spin flip of one of the three electrons mediated by the spin-orbit interaction; in the second step the 3X relaxes to its ground state via phonon emission

GEOMATICS AND CIVIL ENGINEERING INNOVATIVE RESEARCH ON HERITAGE: INTRODUCING THE “ENGINEER” PROJECT

This paper aims to introduce the concept and objectives of a recently supported European project entitled “Geomatics and Civil Engineering Innovative Research on Heritage”, in short ENGINEER. The ENGINEER project visions to enhance and extend inter-departmental multidisciplinary research activities of the Department of Civil Engineering & Geomatics of the Cyprus University of Technology through coordination and support actions as well as through targeted research activities with the support of European leading institutions. Project tasks aim to fill research multidisciplinary gaps, push, and extend knowledge into new and innovative fields dealing with the monitoring, digitization, visualization, and preservation of ancient monuments and cultural heritage sites, assisting their protection, promotion, and safeguarding

Dysfunction of the Intestinal Microbiome in Inflammatory Bowel Disease and Treatment

Background: The inflammatory bowel diseases (IBD) Crohn's disease and ulcerative colitis result from alterations in intestinal microbes and the immune system. However, the precise dysfunctions of microbial metabolism in the gastrointestinal microbiome during IBD remain unclear. We analyzed the microbiota of intestinal biopsies and stool samples from 231 IBD and healthy subjects by 16S gene pyrosequencing and followed up a subset using shotgun metagenomics. Gene and pathway composition were assessed, based on 16S data from phylogenetically-related reference genomes, and associated using sparse multivariate linear modeling with medications, environmental factors, and IBD status. Results: Firmicutes and Enterobacteriaceae abundances were associated with disease status as expected, but also with treatment and subject characteristics. Microbial function, though, was more consistently perturbed than composition, with 12% of analyzed pathways changed compared with 2% of genera. We identified major shifts in oxidative stress pathways, as well as decreased carbohydrate metabolism and amino acid biosynthesis in favor of nutrient transport and uptake. The microbiome of ileal Crohn's disease was notable for increases in virulence and secretion pathways. Conclusions: This inferred functional metagenomic information provides the first insights into community-wide microbial processes and pathways that underpin IBD pathogenesis

Assessing the dynamics and complexity of disease pathogenicity using 4-dimensional immunological data

Investigating disease pathogenesis and personalized prognostics are major biomedical needs. Because patients sharing the same diagnosis can experience different outcomes, such as survival or death, physicians need new personalized tools, including those that rapidly differentiate several inflammatory phases. To address these topics, a pattern recognition-based method (PRM) that follows an inverse problem approach was designed to assess, in <10min, eight concepts: synergy, pleiotropy, complexity, dynamics, ambiguity, circularity, personalized outcomes, and explanatory prognostics (pathogenesis). By creating thousands of secondary combinations derived from blood leukocyte data, the PRM measures synergic, pleiotropic, complex and dynamic data interactions, which provide personalized prognostics while some undesirable features—such as false results and the ambiguity associated with data circularity-are prevented. Here, this method is compared to Principal Component Analysis (PCA) and evaluated with data collected from hantavirus-infected humans and birds that appeared to be healthy. When human data were examined, the PRM predicted 96.9 % of all surviving patients while PCA did not distinguish outcomes. Demonstrating applications in personalized prognosis, eight PRM data structures sufficed to identify all but one of the survivors. Dynamic data patterns also distinguished survivors from non-survivors, as well as one subset of non-survivors, which exhibited chronic inflammation. When the PRM explored avian data, it differentiated immune profiles consistent with no, early, or late inflammation. Yet, PCA did not recognize patterns in avian data. Findings support the notion that immune responses, while variable, are rather deterministic: a low number of complex and dynamic data combinations may be enough to, rapidly, unmask conditions that are neither directly observable nor reliably forecasted.Conacyt of Mexico (Consejo Nacional de Ciencia y Tecnologíahttp://www.frontiersin.org/Immunologyam2020Veterinary Tropical Disease

GEOMATICS AND CIVIL ENGINEERING INNOVATIVE RESEARCH ON HERITAGE: INTRODUCING THE “ENGINEER” PROJECT

This paper aims to introduce the concept and objectives of a recently supported European project entitled “Geomatics and Civil Engineering Innovative Research on Heritage”, in short ENGINEER. The ENGINEER project visions to enhance and extend inter- departmental multidisciplinary research activities of the Department of Civil Engineering & Geomatics of the Cyprus University of Technology through coordination and support actions as well as through targeted research activities with the support of European leading institutions. Project tasks aim to fill research multidisciplinary gaps, push, and extend knowledge into new and innovative fields dealing with the monitoring, digitization, visualization, and preservation of ancient monuments and cultural heritage sites, assisting their protection, promotion, and safeguarding

Big Earth Data for Cultural Heritage in the Copernicus Era

Digital data is stepping in its golden age characterized by an increasing growth of both classical and emerging big earth data along with trans- and multidisciplinary methodological approaches and services addressed to the study, preservation and sustainable exploitation of cultural heritage (CH). The availability of new digital technologies has opened new possibilities, unthinkable only a few years ago for cultural heritage. The currently available digital data, tools and services with particular reference to Copernicus initiatives make possible to characterize and understand the state of conservation of CH for preventive restoration and opened up a frontier of possibilities for the discovery of archaeological sites from above and also for supporting their excavation, monitoring and preservation. The different areas of intervention require the availability and integration of rigorous information from different sources for improving knowledge and interpretation, risk assessment and management in order to make more successful all the actions oriented to the preservation of cultural properties. One of the biggest challenges is to fully involve the citizen also from an emotional point of view connecting “pixels with people” and “bridging” remote sensing and social sensing

Dietary levels of pure flavonoids improve spatial memory performance and increase hippocampal brain-derived neurotrophic factor.

Evidence suggests that flavonoid-rich foods are capable of inducing improvements in memory and cognition in animals and humans. However, there is a lack of clarity concerning whether flavonoids are the causal agents in inducing such behavioral responses. Here we show that supplementation with pure anthocyanins or pure flavanols for 6 weeks, at levels similar to that found in blueberry (2% w/w), results in an enhancement of spatial memory in 18 month old rats. Pure flavanols and pure anthocyanins were observed to induce significant improvements in spatial working memory (p = 0.002 and p = 0.006 respectively), to a similar extent to that following blueberry supplementation (p = 0.002). These behavioral changes were paralleled by increases in hippocampal brain-derived neurotrophic factor (R = 0.46, p<0.01), suggesting a common mechanism for the enhancement of memory. However, unlike protein levels of BDNF, the regional enhancement of BDNF mRNA expression in the hippocampus appeared to be predominantly enhanced by anthocyanins. Our data support the claim that flavonoids are likely causal agents in mediating the cognitive effects of flavonoid-rich foods