Kartläggning av ogräsförekomsten på Mickels hemman i Närpes

Ogräsarternas frekvens och artsammansättning varierar mellan regioner, gårdar och enskilda skiften. Artförekomsten förändras långsamt pga. att många arters frön kan ligga många år i groningsvila i marken. Utan anpassad reglering och bekämpning kan ogräsen bli ett stort problem som påverkar huvudgrödans avkastning. Klimat, jordart, växtföljd, jordbearbetning och direkt riktade bekämpningsåtgärder, är de huvudsakliga faktorerna som inverkar på ogräsflorans sammansättning på fältet. Framgångsrik bekämpning av ogräsen förutsätter kännedom om vilka arter som förekommer på ett enskilt skifte. Detta gäller i synnerhet vid användning av kemiska ogräsbekämpningsmedel. Genom ett odlingsförsök kartlades ogräsfloran på samtliga, till jordbruksföretaget Mickels hörande, skiften. Från varje skifte togs sex jordprov. Jordproverna breddes ut i lådor och de i proven befintliga ogräsfröna stimulerades med hjälp av bevattning, temperatur och konstljus till groning. De uppkomna ogräsplantorna artbestämdes och räknades. 16 olika ogräsarter identifierades, varav åkerviol var den mest frekventa. Resultaten i undersökningen liknade till stor del de resultat som framlagts i tidigare undersökningar i Finland. Kartläggningen antyder att alla fält kan bekämpas med samma på marknaden befintliga herbicidblandning eftersom denna hade god effekt på samtliga av de mest frekventa ogräsen.Weed frequency and species composition vary between regions, farms and individual fields. The occurrence of weed species changes very slowly because a lot of species seeds can be in dormancy in the ground for many years. Without specific regulation and control the weeds can become a large problem, which affects the yield negatively. Climate, soil type, crop rotation, cultivation and directly aimed control measurements are the main factors that affect the weed species composition in the fields. A successful weed control presupposes knowledge of the species occurring in a single field. This applies particularly when chemical weed control is used. By means of a growth trial the weed flora occurring on the fields belonging to the Mickel farm was mapped. Six soil samples were taken from every field. The samples were put in boxes and the existing weed seeds in them were stimulated to germinate by irrigation, temperature and artificial light. The emerging plants were identified by species and counted. Sixteen different weed species were identified, of which field pansy was the most frequent. The results in the investigation largely resembled the results of previous investigations in Finland. The mapping indicates that all the fields can be treated with one specific herbicide mixture existing on the market, as this was effective on the most frequent weed species

Why are Extraverts more Popular? : Oral Fluency mediates the Effect of Extraversion on Popularity in Middle Childhood

Peer reviewe

Is there a g-factor of genderedness? Using a continuous measure of genderedness to assess sex differences in personality, values, cognitive ability, school grades, and educational track

Some of the most persistently recurring research questions concern sex differences. Despite much progress, limited research has thus far been undertaken to investigate whether there is one general construct of genderedness that runs through various domains of human individuality. In order to determine whether being gender typical in one way goes together with being gender typical also in other ways, we investigated whether 16-year-old Finnish girls and boys (N = 4106) differ in their personality, values, cognitive abilities, academic achievement, and educational track. To do this, we updated the prediction-focused gender diagnosticity approach by methods of cross-validation for more accurate estimation. The preregistered analysis shows that sex differences vary across domains (Ds = 0.15–1.48), that fine-grained measures, such as grade profiles, can be accurate in predicting sex (77.5%), whereas some summary indices, such as general cognitive ability, do not perform above-chance (52.4%), and that the genderedness correlations, despite all being positive, are too weak (average partial correlation, r´ = .09, range .03–.34) to support a general factor of genderedness. Our more exploratory analyses show that more focus on gender typicality could offer important insights into the role of gender in shaping people’s lives.publishedVersionPeer reviewe

Attitudes towards genetic testing and information : does parenthood shape the views?

This study examines how parents of pediatric patients might differ in their views and attitudes towards genetic technology and information when compared to adult patients. There is surprisingly little evidence on how parents compare to other parts of population in their attitudes. Previous empirical studies often relate health-related preferences and attitudes to factors such as age, education, and income instead of parental status, thus evading comparison of parents to others as health-related decision makers. Findings related to the parental status can be useful when implementing genetic technology in clinical practice. We conducted a survey of views on genetic technology and information for groups of adult neurology patients (n = 68) and parents of pediatric neurology patients (n = 31) to shed some light on this issue. In addition to our own survey instrument, we conducted other surveys to gain insight on psychosocial factors that might affect these attitudes. The results suggest that parents are more concerned about their children's genetic risk factors when compared to the attitudes of adult patients about their own risk. For both groups, negative emotional state was associated with more concerns towards genetic information. Our study provides insights on how parental views might affect the acceptance of genetic technology and information.Peer reviewe

Diagnostic value of serum biomarkers FGF21 and GDF15 compared to muscle sample in mitochondrial disease

The aim of this study was to compare the value of serum biomarkers, fibroblast growth factor 21 (FGF21) and growth differentiation factor 15 (GDF15), with histological analysis of muscle in the diagnosis of mitochondrial disease. We collected 194 serum samples from patients with a suspected or known mitochondrial disease. Biomarkers were analyzed blinded using enzyme-labeled immunosorbent assay. Clinical data were collected using a structured questionnaire. Only 39% of patients with genetically verified mitochondrial disease had mitochondrial pathology in their muscle histology. In contrast, biomarkers were elevated in 62% of patients with genetically verified mitochondrial disease. Those with both biomarkers elevated had a muscle manifesting disorder and a defect affecting mitochondrial DNA expression. If at least one of the biomarkers was induced and the patient had a myopathic disease, a mitochondrial DNA expression disease was the cause with 94% probability. Among patients with biomarker analysis and muscle biopsy takenPeer reviewe

ATPase-deficient mitochondrial inner membrane protein ATAD3A disturbs mitochondrial dynamics in dominant hereditary spastic paraplegia

De novo mutations in ATAD3A (ATPase family AAA-domain containing protein 3A) were recently found to cause a neurological syndrome with developmental delay, hypotonia, spasticity, optic atrophy, axonal neuropathy, and hypertrophic cardiomyopathy. Using whole-exome sequencing, we identified a dominantly inherited heterozygous variant c.1064G > A (p.G355D) in ATAD3A in a mother presenting with hereditary spastic paraplegia (HSP) and axonal neuropathy and her son with dyskinetic cerebral palsy, both with disease onset in childhood. HSP is a clinically and genetically heterogeneous disorder of the upper motor neurons. Symptoms beginning in early childhood may resemble spastic cerebral palsy. The function of ATAD3A, a mitochondrial inner membrane AAA ATPase, is yet undefined. AAA ATPases form hexameric rings, which are catalytically dependent on the co-operation of the subunits. The dominant-negative patient mutation affects the Walker A motif, which is responsible for ATP binding in the AAA module of ATAD3A, and we show that the recombinant mutant ATAD3A protein has a markedly reduced ATPase activity. We further show that overexpression of the mutant ATAD3A fragments the mitochondrial network and induces lysosome mass. Similarly, we observed altered dynamics of the mitochondrial network and increased lysosomes in patient fibroblasts and neurons derived through differentiation of patient-specific induced pluripotent stem cells. These alterations were verified in patient fibroblasts to associate with upregulated basal autophagy through mTOR inactivation, resembling starvation. Mutations in ATAD3A can thus be dominantly inherited and underlie variable neurological phenotypes, including HSP, with intrafamiliar variability. This finding extends the group of mitochondrial inner membrane AAA proteins associated with spasticity.Peer reviewe

Attitudes towards genetic testing and information: does parenthood shape the views?

This study examines how parents of pediatric patients might differ in their views and attitudes towards genetic technology and information when compared to adult patients. There is surprisingly little evidence on how parents compare to other parts of population in their attitudes. Previous empirical studies often relate health-related preferences and attitudes to factors such as age, education, and income instead of parental status, thus evading comparison of parents to others as health-related decision makers. Findings related to the parental status can be useful when implementing genetic technology in clinical practice. We conducted a survey of views on genetic technology and information for groups of adult neurology patients (n = 68) and parents of pediatric neurology patients (n = 31) to shed some light on this issue. In addition to our own survey instrument, we conducted other surveys to gain insight on psychosocial factors that might affect these attitudes. The results suggest that parents are more concerned about their children's genetic risk factors when compared to the attitudes of adult patients about their own risk. For both groups, negative emotional state was associated with more concerns towards genetic information. Our study provides insights on how parental views might affect the acceptance of genetic technology and information

Implementation of CYP2D6 copy-number imputation panel and frequency of key pharmacogenetic variants in Finnish individuals with a psychotic disorder

We demonstrate that CYP2D6 copy-number variation (CNV) can be imputed using existing imputation algorithms. Additionally, we report frequencies of key pharmacogenetic variants in individuals with a psychotic disorder from the genetically bottle-necked population of Finland. We combined GWAS chip and CYP2D6 CNV data from the Breast Cancer Pain Genetics study to construct an imputation panel (n = 902) for CYP2D6 CNV. The resulting data set was used as a CYP2D6 CNV imputation panel in 9262 non-related individuals from the SUPER-Finland study. Based on imputation of 9262 individuals we confirm the higher frequency of CYP2D6 ultrarapid metabolizers and a 22-fold enrichment of the UGT1A1 decreased function variant rs4148323 (UGT1A1*6) in Finland compared with non-Finnish Europeans. Similarly, the NUDT15 variant rs116855232 was highly enriched in Finland. We demonstrate that imputation of CYP2D6 CNV is possible and the methodology enables studying CYP2D6 in large biobanks with genome-wide data.Peer reviewe

Implementation of CYP2D6 copy-number imputation panel and frequency of key pharmacogenetic variants in Finnish individuals with a psychotic disorder

We demonstrate that CYP2D6 copy-number variation (CNV) can be imputed using existing imputation algorithms. Additionally, we report frequencies of key pharmacogenetic variants in individuals with a psychotic disorder from the genetically bottle-necked population of Finland. We combined GWAS chip and CYP2D6 CNV data from the Breast Cancer Pain Genetics study to construct an imputation panel (n = 902) for CYP2D6 CNV. The resulting data set was used as a CYP2D6 CNV imputation panel in 9262 non-related individuals from the SUPER-Finland study. Based on imputation of 9262 individuals we confirm the higher frequency of CYP2D6 ultrarapid metabolizers and a 22-fold enrichment of the UGT1A1 decreased function variant rs4148323 (UGT1A1*6) in Finland compared with non-Finnish Europeans. Similarly, the NUDT15 variant rs116855232 was highly enriched in Finland. We demonstrate that imputation of CYP2D6 CNV is possible and the methodology enables studying CYP2D6 in large biobanks with genome-wide data.</p

No Reliable Association between Runs of Homozygosity and Schizophrenia in a Well-Powered Replication Study

It is well known that inbreeding increases the risk of recessive monogenic diseases, but it is less certain whether it contributes to the etiology of complex diseases such as schizophrenia. One way to estimate the effects of inbreeding is to examine the association between disease diagnosis and genome-wide autozygosity estimated using runs of homozygosity (ROH) in genome-wide single nucleotide polymorphism arrays. Using data for schizophrenia from the Psychiatric Genomics Consortium (n = 21,868), Keller et al. (2012) estimated that the odds of developing schizophrenia increased by approximately 17% for every additional percent of the genome that is autozygous (β = 16.1, CI(β) = [6.93, 25.7], Z = 3.44, p = 0.0006). Here we describe replication results from 22 independent schizophrenia case-control datasets from the Psychiatric Genomics Consortium (n = 39,830). Using the same ROH calling thresholds and procedures as Keller et al. (2012), we were unable to replicate the significant association between ROH burden and schizophrenia in the independent PGC phase II data, although the effect was in the predicted direction, and the combined (original + replication) dataset yielded an attenuated but significant relationship between Froh and schizophrenia (β = 4.86,CI(β) = [0.90,8.83],Z = 2.40,p = 0.02). Since Keller et al. (2012), several studies reported inconsistent association of ROH burden with complex traits, particularly in case-control data. These conflicting results might suggest that the effects of autozygosity are confounded by various factors, such as socioeconomic status, education, urbanicity, and religiosity, which may be associated with both real inbreeding and the outcome measures of interest