Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization.

In this protocol, we provide detailed instructions to desensitize human and murine mast cells (MCs) after polyclonal IgE sensitization. Moreover, we specify the steps for MC degranulation assessment after desensitization, measuring CD63 and CD107a expression by flow cytometry and b-hexosaminidase activity. Desensitized MCs can be used directly for co-culture with other cell types, immunofluorescence, live imaging, and omics approaches.post-print3350 K

Local inflammation enables a basophil-neuronal circuITCH in atopic dermatitis.

post-print1573 K

El flamenco como vehículo comunicativo entre culturas: La imagen del flamenco en la prensa japonesa.

España y Japón son dos países separados por 10.642 kilómetros que poseen un sinfín de diferencias como su lenguaje, sus costumbres o su entorno. Sin embargo, la pasión de la población japonesa por uno de los bienes culturales españoles más conocidos, el flamenco, es latente desde años antes de la dictadura franquista. Este estudio trata de conocer las relaciones entre España y Japón gracias al flamenco y de estudiar cuál es la verdadera relevancia que posee este bien cultural español en el país nipón. Para ello, se va a analizar qué importancia otorga la prensa digital japonesa al flamenco a través de su contenido. <br /

Meglumine gadoterate induces immunoglobulin-independent human mast cell activation via MRGPRX2.

post-print2297 K

Abordaje fisioterápico en una niña con síndrome de Angelman: a propósito de un caso

Introducción. El síndrome de Angelman es un trastorno del neurodesarrollo que se caracteriza por un retraso psicomotor acompañado de trastornos en el movimiento, un profundo retraso cognitivo con graves dificultades de aprendizaje, habla muy limitada, conducta hiperactiva y risa frecuente, producida por una alteración genética en el que se ve involucrado un cromosoma materno. Objetivos. Los objetivos se centran en estimular el desarrollo psicomotor tratando de conseguir la máxima independencia, evitando a su vez la aparición de deformidades, ayudándose del uso de dispositivos ortopédicos. Metodología. Se aplica un diseño AB longitudinal prospectivo. El sujeto de estudio es una niña de 4 años de edad, diagnosticado de síndrome de Angelman, que se manifiesta clínicamente con retraso psicomotor, inestabilidad en el equilibrio estático y dinámico y alteraciones ortopédicas, afectando a su autonomía. Se realiza una valoración fisioterápica inicial para la consiguiente elaboración del plan de intervención fisioterápico, utilizando distintos dispositivos ortopédicos. Desarrollo. Se aplica el tratamiento fisioterápico 3 veces por semana durante 6 meses, observándose en los resultados de la valoración final una mejoría a nivel motor y cognitivo junto con una mayor autonomía. Conclusiones. A pesar de que los resultados no son generalizables, el plan de intervención fisioterápico propuesto muestra mejoras en la evolución de la paciente, aunque sería conveniente hacer más estudios para encontrar evidencia de otras vías de tratamiento, dado que es un síndrome poco frecuente. Palabras clave: síndrome de Angelman, hiperactividad, dispositivos ortopédicos

The impact of type 2 immunity and allergic diseases in atherosclerosis.

Allergic diseases are allergen-induced immunological disorders characterized by the development of type 2 immunity and IgE responses. The prevalence of allergic diseases has been on the rise alike cardiovascular disease (CVD), which affects arteries of different organs such as the heart, the kidney and the brain. The underlying cause of CVD is often atherosclerosis, a disease distinguished by endothelial dysfunction, fibrofatty material accumulation in the intima of the artery wall, smooth muscle cell proliferation, and Th1 inflammation. The opposed T-cell identity of allergy and atherosclerosis implies an atheroprotective role for Th2 cells by counteracting Th1 responses. Yet, the clinical association between allergic disease and CVD argues against it. Within, we review different phases of allergic pathology, basic immunological mechanisms of atherosclerosis and the clinical association between allergic diseases (particularly asthma, atopic dermatitis, allergic rhinitis and food allergy) and CVD. Then, we discuss putative atherogenic mechanisms of type 2 immunity and allergic inflammation including acute allergic reactions (IgE, IgG1, mast cells, macrophages and allergic mediators such as vasoactive components, growth factors and those derived from the complement, contact and coagulation systems) and late phase inflammation (Th2 cells, eosinophils, type 2 innate-like lymphoid cells, alarmins, IL-4, IL-5, IL-9, IL-13 and IL-17).Severo Ochoa Center of Excellence, Grant/Award Number: CEX2020-001041- S; Pro CNIC Foundation; Ministerio de Ciencia e Innovación; Ministry of Science and Innovation, Grant/ Award Number: PID2019-110369RB- I00; European Commission, Grant/Award Number: ERC-CoG 819775 and H2020-HEALTH 945118; Spanish Ministry of Universities; Ayudas Margarita Salas para la Formación de Jóvenes Doctores—Universidad Autónoma de Madrid, Grant/ Award Number: CA1/RSUE/2021–00577; Formación de Profesorado Universitario, Grant/Award Number: FPU16/03953; Sociedad Española de Alergología e Inmunología Clínica (SEAIC), Grant/ Award Number: BECA20A9; New Frontiers in Research Fund, Grant/ Award Number: NFRFE-2019- 00083; The Nutricia Research Foundation, Grant/Award Number: NRF-2021- 13; Instituto de Salud Carlos III, Grant/Award Number: PI21/00158, PI21/01126, CP20/00043, PI18/01467, PI19/00044, RD16/0006/0015 and RD21/0002/0008; Severo Ochoa Program, Grant/Award Number: AEI/SEV-2017- 0712S

Amyloid β / PKC-dependent alterations in NMDA receptor composition are detected in early stages of Alzheimer´s disease

[EN] Amyloid beta (Abeta)-mediated synapse dysfunction is an early event in Alzheimer's disease (AD) pathogenesis and previous studies suggest that NMDA receptor (NMDAR) dysregulation may contribute to these pathological effects. Although Abeta peptides impair NMDAR expression and activity, the mechanisms mediating these alterations in the early stages of AD are unclear. Here, we observed that NMDAR subunit NR2B and PSD-95 levels were aberrantly upregulated and correlated with Abeta42 load in human postsynaptic fractions of the prefrontal cortex in early stages of AD patients, as well as in the hippocampus of 3xTg-AD mice. Importantly, NR2B and PSD95 dysregulation was revealed by an increased expression of both proteins in Abeta-injected mouse hippocampi. In cultured neurons, Abeta oligomers increased the NR2B-containing NMDAR density in neuronal membranes and the NMDA-induced intracellular Ca2+ increase, in addition to colocalization in dendrites of NR2B subunit and PSD95. Mechanistically, Abeta oligomers required integrin beta1 to promote synaptic location and function of NR2B-containing NMDARs and PSD95 by phosphorylation through classic PKCs. These results provide evidence that Abeta oligomers modify the contribution of NR2B to NMDAR composition and function in the early stages of AD through an integrin beta1 and PKC-dependent pathway. These data reveal a novel role of Abeta oligomers in synaptic dysfunction that may be relevant to early-stage AD pathogenesis.We thank S. Marcos, L. Escobar, A Martínez and Z. Martínez for technical assistance. This study was supported by the Basque Government (IT1203-19; PIBA_2020_1_0012; ELKARTEK KK-2020/00034; fellowship to T.Q-L, U.B. and J.Z-I), University of the Basque Country (UPV/EHU; fellowship to C.O-S) CIBERNED, MICINN (PID2019-108465RB-I00) and Fundación Tatiana Pérez de Guzmán el Bueno (fellowship to C.L)

A Differential Signature of Circulating miRNAs and Cytokines Between COVID-19 and Community-Acquired Pneumonia Uncovers Novel Physiopathological Mechanisms of COVID-19

Coronavirus Disease 2019 (COVID-19) pneumonia is a life-threatening infectious disease, especially for elderly patients with multiple comorbidities. Despite enormous efforts to understand its underlying etiopathogenic mechanisms, most of them remain elusive. In this study, we compared differential plasma miRNAs and cytokines profiles between COVID-19 and other community-acquired pneumonias (CAP). A first screening and subsequent validation assays in an independent cohort of patients revealed a signature of 15 dysregulated miRNAs between COVID-19 and CAP patients. Additionally, multivariate analysis displayed a combination of 4 miRNAs (miR-106b-5p, miR-221-3p, miR-25-3p and miR-30a-5p) that significantly discriminated between both pathologies. Search for targets of these miRNAs, combined with plasma protein measurements, identified a differential cytokine signature between COVID-19 and CAP that included EGFR, CXCL12 and IL-10. Significant differences were also detected in plasma levels of CXCL12, IL-17, TIMP-2 and IL-21R between mild and severe COVID-19 patients. These findings provide new insights into the etiopathological mechanisms underlying COVID-19This study was funded by Spanish Ministry of Economy, Industry and Competitiveness (MINECO) and Instituto de Salud Carlos III (grant nos. RD16/0011/0012 and PI18/0371 to IG-Á, grant no. PI19/00549 to AA, and grant no. PDI-2020-120412RB-I00 to FSM) and co-funded by the European Regional Development Fund. The study was also funded by ”La Caixa Banking Foundation” (grant no. HR17-00016 to FSM), REACT-UE INMUNOVACTER-CM from Comunidad de Madrid, and ”Fondos Supera COVID19” by Banco de Santander and CRUE. The work of ERV has been funded by a Rio-Hortega grant from the Ministerio de Economı́a y Competitividad (grant no. CM19/00149 Instituto de Salud Carlos III) and co-funded by The European Regional Development Fund (ERDF) “A way to make Europe

The impact of type 2 immunity and allergic diseases in atherosclerosis.

Allergic diseases are allergen-induced immunological disorders characterized by the development of type 2 immunity and IgE responses. The prevalence of allergic diseases has been on the rise alike cardiovascular disease (CVD), which affects arteries of different organs such as the heart, the kidney and the brain. The underlying cause of CVD is often atherosclerosis, a disease distinguished by endothelial dysfunction, fibrofatty material accumulation in the intima of the artery wall, smooth muscle cell proliferation, and Th1 inflammation. The opposed T-cell identity of allergy and atherosclerosis implies an atheroprotective role for Th2 cells by counteracting Th1 responses. Yet, the clinical association between allergic disease and CVD argues against it. Within, we review different phases of allergic pathology, basic immunological mechanisms of atherosclerosis and the clinical association between allergic diseases (particularly asthma, atopic dermatitis, allergic rhinitis and food allergy) and CVD. Then, we discuss putative atherogenic mechanisms of type 2 immunity and allergic inflammation including acute allergic reactions (IgE, IgG1, mast cells, macrophages and allergic mediators such as vasoactive components, growth factors and those derived from the complement, contact and coagulation systems) and late phase inflammation (Th2 cells, eosinophils, type 2 innate-like lymphoid cells, alarmins, IL-4, IL-5, IL-9, IL-13 and IL-17).N

Deregulated cellular circuits driving immunoglobulins and complement consumption associate with the severity of COVID-19 patients

SARS-CoV-2 infection causes an abrupt response by the host immune system, which is largely responsible for the outcome of COVID-19. We investigated whether the specific immune responses in the peripheral blood of 276 patients were associated with the severity and progression of COVID-19. At admission, dramatic lymphopenia of T, B, and NK cells is associated with severity. Conversely, the proportion of B cells, plasmablasts, circulating follicular helper T cells (cTfh) and CD56–CD16+ NK-cells increased. Regarding humoral immunity, levels of IgM, IgA, and IgG were unaffected, but when degrees of severity were considered, IgG was lower in severe patients. Compared to healthy donors, complement C3 and C4 protein levels were higher in mild and moderate, but not in severe patients, while the activation peptide of C5 (C5a) increased from the admission in every patient, regardless of their severity. Moreover, total IgG, the IgG1 and IgG3 isotypes, and C4 decreased from day 0 to day 10 in patients who were hospitalized for more than two weeks, but not in patients who were discharged earlier. Our study provides important clues to understand the immune response observed in COVID-19 patients, associating severity with an imbalanced humoral response, and identifying new targets for therapeutic interventionThe study was funded by grants SAF2017- 82886-R to FS-M from the Ministerio de Economía y Competitividad, and from “La Caixa Banking Foundation” (HR17-00016) to FS-M. Grant PI018/01163 to CMC and grant PI19/00549 to AA were funded by Fondo de Investigaciones Sanitarias, Ministerio de Sanidad y Consumo, Spain. SAF2017-82886-R, PI018/01163 and PI19/00549 grants were also co-funded by European Regional Development Fund, ERDF/FEDER. This work has been funded by grants Fondo Supera COVID (CRUE-Banco de Santander) to FSM, and “Ayuda Covid 2019” from Comunidad de Madri