Gavidia, Una Conducta

Gavidia, Una Conduct

Rildo: Real-World Multicenter Study on the Effectiveness and Safety of Single-Tablet Regimen of Dolutegravir plus Rilpivirine in Treatment-Experienced People Living with HIV

Two-drug regimens (2DRs) are emerging in clinical practice guidelines as treatment option for both naive and treatment-experienced people living with HIV (PLHIV). Objectives: To determine the real-life effectiveness of 2DR with 25 mg RPV plus 50 mg DTG in a single-tablet regimen (RPV/DTGSTR) and its impact on viral and immune status, lipid profile, and inflammatory markers. Methods: This observational study included 291 treatment-experienced PLHIV, starting 2DR with RPV/DTGSTR between 29 January 2019 and 2 February 2022, who were followed up for at least six months. Participants gave verbal informed consent for the switch in antiretroviral therapy (ART) to RPV/DTGSTR. Results: The mean age of the 291 participants was 51.3 years; 77.7% were male; and 42.9% were in the AIDS stage with a CD4 nadir of 283.5 ± 204.6 cells/uL. The median time since HIV diagnosis was 19.7 years (IQR: 10.6-27). Before 2DR, patients received a median of five ART lines (IQR: 3-7) for 22.2 years (IQR: 14-26), with 34.4% (n = 100) receiving a three-drug regimen (3DR), 31.3% (n = 91) receiving monotherapy, and 34.4% (n = 100) receiving 2DR. The median time on RPV/DTGSTR was 14 months (IQR: 9.5-21); 1.4% were lost to the follow-up. Effectiveness was 96.2% by intention-to-treat (ITT) analysis, 97.5% by modified ITT, and 99.3% by per-protocol analysis. Virological failure was observed in 0.69%, blips in 3.5%, and switch to another ART in 1.4%. The mean lipid profile improved, with reductions in TC/HDLc ratio (3.9 ± 0.9 vs. 3.6 ± 0.9; p = 0.0001), LDLc (118.3 ± 32.2 mg/dL vs. 106.2 ± 29.8 mg/dL, p = 0.0001), TG (130.9 ± 73.9 mg/dL vs. 115.9 ± 68.5 mg/dL, p = 0.0001), and CD4/CD8 ratio increase (0.99 ± 0.58 vs. 1.01 ± 0.54; p = 0.0001). The cost-effectiveness of 2DR with RPV/DTGSTR was similar to that of DTG/3TC and superior to those of BIC/TAF/FTC and DRV/c/TAF/FTC, with higher virological suppression and lower annual costs. Conclusions: The switch to RPV plus DTG in STR is a cost-effective, long-lasting, and robust strategy for PLHIV, with a very long experience of treatment, which improves the lipid profile without affecting inflammatory markers

Phenotypic and genetic components for growth, morphology, and flesh-quality traits of meagre (Argyrosomus Regius) reared in tank and sea cage

Meagre (Argyrosomus regius) plays an important role in the aquaculture system, with the potential for diversification of European aquaculture, and is characterized by its fast growth rate, low feed conversion ratio, and the high quality of the product. Focusing on the relevance of meagre, the aim of the study was to analyze growth performance, fish morphology, and flesh composition phenotypically and genetically to be considered as a strategy in a breeding program. For this purpose, 633 fish were raised in two different housing systems, in sea cages or in a continental tank, and when they reached harvest size, manual growth traits, automatic morphology by the image analysis program IMAFISH_ML, and flesh chemical composition (fat, protein, moisture, and collagen percentages) were measured. The fish reared in the cages showed a higher body weight and fillet fat percentage than those in the tank. Heritabilities for growth and morphology traits, and for fillet fat percentage were medium, revealing these traits as a possible selection criterion in a breeding program. Phenotypic and genetic correlations between growth and morphology traits were positive and high. Phenotypic correlations between growth or morphology traits with fillet fat percentage were positive and medium; genetic correlations were not estimated accurately.This research was funded by Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia through the project “Mejora de la competitividad del sector de la corvina a través de la selección genética” (GENECOR, 21002/PI/18) in the call for grants for projects for the development of scientific and technical research by competitive groups, included in the Regional Program for the Promotion of Scientific and Technical Research (Action Plan 2018). A.V. was funded by a pre-doctoral research fellowship (20716/FPI/18. Fundación Séneca. Cofinanciado por grupo Avramar S.L. Región de Murcia (Spain))

Development of the first microsatellite multiplex PCR panel for meagre (Argyrosomus regius), a commercial aquaculture species

In this study, a microsatellite-based multiplex PCR panel for meagre (Argyrosomus regius) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre (Argyrosomus regius), Japanese meagre (A. japonicus), red drum (Sciaenops ocellatus) and yellow meagre (Acoupa weakfish) were assessed for genetic variability, allelic range and genotype reliability. Finally, a SuperMultiplex for Argyrosomus regius (SMAr) was designed with only the best eight microsatellite markers. The panel assessment was performed using a batch of brood stock from one company and a sample of 616 offspring. It was possible to assign 95% of the offspring to a single pair of parents using the exclusion method. It is therefore considered an easy procedure, and a powerful and low-cost tool for parental assignment to support companies’ breeding programs and to exchange information between research groups.This research was funded by Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia through the project “Mejora de la competitividad del sector de la corvina a través de la selección genética (GENECOR, 21002/PI/18)” in the call for grants for projects for the development of scientific and technical research by competitive groups, included in the Regional Program for the Promotion of Scientific and Technical Research (Action Plan 2018). A.V. was funded by a pre-doctora research fellowship (20716/FPI/18. Fundación Séneca. Cofinanciado por grupo Avramar S.L. Región de Murcia (Spain)). The Avramar S.L. group provided financial support and the use of its facilities for this study

Genetic parameters for Photobacterium damselae subsp. piscicida resistance, immunological markers and body weight in gilthead seabream (Sparus aurata)

A challenge test for Photobacterium damselae subsp. piscicida (Phdp) resistance was carried out in two juvenile populations of gilthead seabream (Sparus aurata L.): F2_ATL and F0_MED. At 250 days post-hatching (dph), a fish plasma sample was collected to measure humoral immune markers (peroxidase activity, bactericidal activity, and IgM immunoglobulin levels), and at 272 dph fish were weighed and inoculated with bacteria Phdp. From that time onwards, surviving fish were recorded for nine days, and days to death was registered. Heritabilities for body weight and Phdp survival were moderate, although for days to death the heritability was low. Regarding humoral immune markers, for peroxidase activity it was moderate, and for IgM levels and for bactericidal activity it was low. Genetic correlations for body weight with Phdp survival and days to death were high and positive, while with peroxidase activity and IgM levels they tended to be positive, although these estimates were not accurate. Regarding genetic correlations between Phdp survival and humoral immune markers, they were very high, positive with peroxidase activity, and negative with IgM levels and bactericidal activity. Some humoral immune markers, particularly peroxidase activity, along with performance traits such as body weight and absence of deformities, are proposed to be included in a selective breeding program to raise fish that are capable of coping with diseasesThis project has received funding from the European Maritime and Fisheries Fund (EMFF) through the PROGENSA III project (Mejora de la Competitividad del Sector de la Dorada a Través de la Selección Genética, JACUMAR program), This study was also funded by Fondo Europeo Marítimo y de Pesca (FEMP) code PR.FEM.PPA201700.14. R.P. was supported by Universidad Politécnica de Cartagena Research Grant; A.V. was funded by a research scholarship fellow (Mejora de la Competitividad del Sector de la Dorada a Través de la Selección Genética, specialization scholarship); AV. was funded by a pre-doctoral research fellow (20,716/FPI/18. Fundación Séneca. Cofinanciado por grupo Andromeda. Región de Murcia (Spain)). Thanks to Javier Lopez Ales and M.A. Castaño Remeseiro for laboratory assistance

Genetic Analysis of the Fatty Acid Profile in Gilthead Seabream (Sparus aurata L.).

The gilthead seabream is one of the most valuable species in the Mediterranean basin both for fisheries and aquaculture. Marine fish, such as gilthead seabream, are a source of n3 polyunsaturated fatty acids, highly appreciated for human food owing to their benefits on the cardiovascular and immune systems. The aim of the present study was to estimate heritability for fatty acid (FA) profile in fillet gilthead seabream to be considered as a strategy of a selective breeding program. Total of 399 fish, from a broodstock Mediterranean Sea, were analysed for growth, flesh composition and FA profile. Heritabilities for growth traits, and flesh composition (fat, protein, and moisture content) were medium. Heritability was moderate for 14:0, 16:0 and 18:1n9 and for sum of monounsaturated FA and n6/n3 ratio, and it was low for 20:1n11 and 22:6n3 and the ratio unsaturated/saturated FA. Breeding programs in gilthead seabream usually include growth as the first criterion in the selection process of the fish. However, other quality traits, such as fillet fat content and its fatty acids profile should be considered, since they are very important traits for the consumer, from a nutritional point of view and the benefits for the health.Versión del edito

Development of the first standardized panel of microsatellites PCR multiplex for meagre (Argyrosomus regius)

[SPA] La realización del panel de microsatélites fue llevada a cabo sobre una población de corvina (Argyrosomus regius) procedente de un lote de reproductores de la empresa Alevines del Sureste S.L. A los 220 días después de la eclosión, los peces fueron muestreados y, momento en el cual, se recogió una muestra de aleta caudal para la posterior extracción de ADN. El objetivo del presente proyecto es establecer un programa de mejora genética en corvina. Se trabajó con un lote de 100 individuos, con los que se comprobaron diversos marcadores microsatélites específicos e interespecíficos. Finalmente, 10 marcadores microsatélites formaron nuestro panel estandarizado para corvina, con una longitud de entre 70-270 pares de bases. [ENG] The microsatellite panel was carried out on a population of meagre (Argyrosomus regius) from a batch of broodstock from the Alevines del Sureste S.L. company, 220 days post-hatching, the fish were weighed and measured, at which time a sample was taken from the tail fin for subsequent DNA extraction. The objective of this project is to establish a breeding program in meagre. We worked with a batch of 100 individuals, with which they were verified in various specific and interspecific microsatellite markers. Finally, 10 microsatellite markers formed our standardized panel for meagre, with a length of between 70-270 base pairs.A la fundación SÉNECA+ Por la beca predoctoral 20716/FPI/18 y al proyecto GENECOR. Cofinanciado por grupo Avramar. Región de Murcia (Spain)