Vitamin C, From Supplement to Treatment: A Re-Emerging Adjunct for Cancer Immunotherapy?

Vitamin C (VitC), in addition to its role as a general antioxidant, has long been considered to possess direct anti-cancer activity at high doses. VitC acts through oxidant and epigenetic mechanisms, which at high doses can exert direct killing of tumor cells in vitro and delay tumor growth in vivo. Recently, it has also been shown that pharmacologic-dose VitC can contribute to control of tumors by modulating the immune system, and studies have been done interrogating the role of physiologic-dose VitC on novel adoptive cellular therapies (ACTs). In this review, we discuss the effects of VitC on anti-tumor immune cells, as well as the mechanisms underlying those effects. We address important unanswered questions concerning both VitC and ACTs, and outline challenges and opportunities facing the use of VitC in the clinical setting as an adjunct to immune-based anti-cancer therapies

A doubly responsive probe for the detection of Cys4-tagged proteins

International audienceRecombinant proteins bearing a tag are crucial tools for assessing protein location or function. Small tags such as Cys4 tag (tetracysteine; Cys–Cys–X–X–Cys–Cys) are less likely disrupt protein function in the living cell than green fluorescent protein. Herein we report the first example of the design and synthesis of a dual fluorescence and hyperpolarized 129Xe NMR-based sensor of Cys4-tagged proteins. This sensor becomes fluorescent when bound to such Cys4-tagged peptides, and the 129Xe NMR spectrum exhibits a specific signal, characteristic of the biosensor-peptide association

VIII. Phénomènes intellectuels. - Pensée et attitudes mentales. - Logique.

Michotte A., Piéron Henri, Duprat G. L., F. E. VIII. Phénomènes intellectuels. - Pensée et attitudes mentales. - Logique.. In: L'année psychologique. 1912 vol. 19. pp. 449-463

Faculté de droit de Paris. De la Condition du prodigue, dans le droit romain, le droit français et dans les législations étrangères modernes. Thèse pour le doctorat par E.-M.-Léonce Delaporte...

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VIII. Phénomènes intellectuels. - Pensée et attitudes mentales. - Logique.

Michotte A., Piéron Henri, Duprat G. L., F. E. VIII. Phénomènes intellectuels. - Pensée et attitudes mentales. - Logique.. In: L'année psychologique. 1912 vol. 19. pp. 449-463

¹²⁹Xe ultra-fast Z spectroscopy enables micromolar detection of biosensors on a 1 T benchtop spectrometer

The availability of a benchtop nuclear magnetic resonance (NMR) spectrometer, of low cost and easily transportable, can allow detection of low quantities of biosensors, provided that hyperpolarized species are used. Here we show that the micromolar threshold can easily be reached by employing laser-polarized xenon and cage molecules reversibly hosting it. Indirect detection of caged xenon is made via chemical exchange, using ultra-fast Z spectroscopy based on spatio-temporal encoding. On this non-dedicated low-field spectrometer, several ideas are proposed to improve the signal.</p

A doubly responsive probe for the detection of Cys4-tagged proteins

International audienceIntroduction Full understanding of intracellular phenomena involves sensitive and non-invasive detection. A less disruptive method than labeling of fluorescent proteins uses binding between a tag of only six natural amine acids that can be genetically incorporated into the protein of interest and a small molecule ca lied FIAsH[1]. This molecule has the ability to fluoresce only when it binds to its 4Cys-tag target. Another technique based on 129Xe NMR has emerged. Xenon is hyperpolarized to enhance the NMR signal by orders of magnitude and its reversible encapsulation in functionalized host systems gives it a specifie spectral signature[2]. Capability of the noble gas to cross cell membranes without losing its polarization[3] enables in cellule investigations. Here we report the first design and study of a dual fluorescence-and 129Xe NMR-based sensor of Cys4-tagged proteins[4]

Synthesis and cytotoxic activity of pyranocoumarins of the seselin and xanthyletin series

The synthesis of known (3-6) and new (7-10 and 14-22) coumarins in the seselin and xanthyletin series is described. The cytotoxic activity of compounds 3-22 was carried out in vitro on L-1210 cells. The most active compounds were 9, 16, 18, and 20 in the seselin series and 10, 17, and 19 in the xanthyletin series. Structure-activity relationships are discussed