Influencia de factores productivos de cabras de raza Florida, en los resultados de fertilidad a inseminación artificial

Este estudio se ha realizado utilizando los datos recogidos por los técnicos de ACRIFLOR y del Centro IFAPA de Hinojosa del Duque durante las anualidades 2005, 2006, 2007 y 2008. Dichos datos informan acerca de la inseminación de 1.739 cabras de la raza Florida. El trabajo se ha realizado con semen fresco refrigerado, estudiando los siguientes factores: Número de parto de la cabra en el momento inseminación, Nivel de producción (kg de leche en el control lechero más cercano) y días de lactación desde la fecha del último parto. La obtención y procesamiento del semen se realiza por técnicos del Instituto Andaluz de Investigación y Formación Agraria, Pesca, Alimentación y de la Producción Ecológica IFAPA en el centro de Recogida de Esperma Caprino ubicado en Hinojosa del Duque (Córdoba), y donde ACRIFLOR, mediante Convenio suscrito con el IFAPA, mantiene su Centro de Testaje de Machos. Las hembras inseminadas deben cumplir los requisitos de: una ecografía previa para descartar las pseudogestaciones, que no hayan abortado o presenten problemas reproductivos, que no sean nulíparas y, además, todas las cabras deben estar identificadas individualmente y en control lechero oficial. Los resultados de fertilidad con inseminación artificial en la raza Florida no han sido tan altos como los resultados obtenidos en otras razas caprinas, los cuales rondan un 60% de fertilidad en semen fresco y un 50% de fertilidad en semen congelado, es por ello que se han analizado los posibles factores que pueden estar provocando estos resultados menos favorables

Evaluation of the small ruminants cystic ovarian disease using transrectal ultrasonography

A transrectal ultrasonic method using a 7.5 MHz linear-array transducer was used in 194 ewes and 39 goats to study the characteristc of cystic ovaries. Cystic structures found on the ovaries include follicular cysts and luteinised cysts. Follicular cysts showed up as non-echogenic structures with a 1.5 ± 0.4 cm diameter. The cysts luteinised showed an echogenic rim of tissue, ranging from 2- 3 mm, around the cyst; the central cavity was 1±0.2 cm. In total, 4.1 percent of ewes and 5.1 percent of goats exhibited follicular cysts. Only 1 percent of ewes and 2.6 percent of goats showed luteinised cysts. In conclusion, the results demonstrated that transrectal ultrasonography provides a noninvasive form of visual access to the ovary from small ruminants, to evaluate pathological changes before the aplication of superovulation treatments and embryo transfer.Se ha realizado un estudio de las características ecográficas de estructuras quísticas ováricas en 194 ovejas y 39 cabras de diferentes razas y procedentes de distintas zonas geográficas, con el objeto de valorar la ecografía transrectal con sondas de alta resolución (7,5 MHz) como criterio de selección de animales destinados a programas de superovulación y recogida de embriones. Los quistes foliculares tenían una incidencia de presentación del 4,1 p.100 en las ovejas y del 5,1 p.100 en las cabras, presentándose ecográficamente con una morfología esférica anecoica de 1,5±0,4 cm de diámetro, con paredes finas y generalmente bien definidas. Los quistes luteínicos tuvieron una presentación del 1 p.100 en las ovejas y del 2,6 p.100 en las cabras. Ecográficamente se caracterizaban por presentar una cavidad central anecógena 1±0,2 cm, rodeada por un anillo ecogénico de 2-3 mm de espesor. No había diferencias significativas en la frecuencia de presentación de quistes ováricos entre ambas especies. Los resultados muestran que la ecografía transrectal con sondas de alta resolución permite la realización de un diagnóstico eficaz de la patología quística ovárica en los pequeños rumiantes, siendo recomendable esta metodología en la selección de hembras donantes y receptoras

Disparate Population and Holobiont Structure of Pocilloporid Corals Across the Red Sea Gradient Demonstrate Species-Specific Evolutionary Trajectories

Global habitat degradation heightens the need to better understand patterns of genetic connectivity and diversity of marine biota across geographical ranges to guide conservation efforts. Corals across the Red Sea are subject to pronounced environmental differences, but studies so far suggest that animal populations are largely connected, excepting evidence for a genetic break between the northern-central and southern regions. Here, we investigated population structure and holobiont assemblage of two common pocilloporid corals, Pocillopora verrucosa and Stylophora pistillata, across the Red Sea. We found little evidence for population differentiation in P. verrucosa, except for the southernmost site. Conversely, S. pistillata exhibited a complex population structure with evidence for within-reef and regional genetic differentiation, in line with differences in their reproductive mode (P. verrucosa is a broadcast spawner and S. pistillata is a brooder). Analysis for genomic loci under positive selection identified 85 sites (18 of which were in coding sequences) that distinguished the southern P. verrucosa population from the remainder of the Red Sea population. By comparison, we found 128 loci (24 of which were residing in coding sequences) in S. pistillata with evidence for local adaptation at various sites. Functional annotation of the underlying proteins revealed putative roles in the response to stress, lipid metabolism, transport, cytoskeletal rearrangement, and ciliary function (among others). Microbial assemblages of both coral species showed pervasive association with microalgal symbionts from the genus Symbiodinium (former clade A) and bacteria from the genus Endozoicomonas that exhibited significant differences according to host genotype and environment. The disparity of population genetic and holobiont assemblage patterns even between closely related species (family Pocilloporidae) highlights the need for multispecies investigations to better understand the role of the environment in shaping evolutionary trajectories. It further emphasizes the importance of networks of reef reserves to achieve conservation of genetic variants critical to the future survival of coral ecosystems

BRIEF UPDATE ON THE SATELLITE TAGGING OF ATLANTIC SWORDFISH

This paper provides a brief update of the study on habitat use for swordfish, developed within the working plan of the Swordfish Species Group of ICCAT. A total of 9 miniPAT tags have been deployed by observers on Portuguese and Spanish vessels and the Uruguayan research cruise in the North and South Atlantic. Data from eight tags/specimens are available, four specimens suffered from post-release mortality and one individual tag pop-up date has not occurred yet. These preliminary results showed swordfish moved in several directions, travelling considerable distances. Swordfish spent most of the daytime in deeper waters, being closer to the surface during night-time. The main plan for the next phase of the project is to continue the tag deployment during 2020 in several regions of the Atlantic Ocean and Mediterranean SeaEn prens

Does Size Matters? Relationships among Social Dominance and Some Morphometric Traits upon Out-of-Season Reproductive Outcomes in Anestrus Dairy Goats Treated with P4 + eCG

The possible role of the social rank (R) (i.e., low-LSR, middle-MSR, or high-HSR) in anestrus goats exposed to a P4 + eCG (D) (i.e., 100 or 350 IU) estrus induction protocol (EIP) was evaluated. Goats (Alpine-Saanen-Nubian × Criollo; n = 70; 25° North) managed under stall-fed conditions were all ultrasound evaluated to confirm anestrous status, while the social rank was determined 30 d prior to the EIP. The response variables included estrus induction (EI, %), duration of estrus (DUR, h), ovulation rate (OR, n), live weight (LW, kg), thoracic perimeter (TP, cm), thoracic diameter (TD, cm), body length (BL, cm), height at withers (HW, cm), beard length (BEA, cm), compactness index (COM, cm), and anamorphosis index (ANA, cm), as affected by R, D, and the R × D interaction were evaluated, while the correlation coefficients among reproductive and morphometric variables were quantified. An R × D interaction (p 0.05) with any response variable, either reproductive or morphometric. As expected, LW had high correlation coefficients (p < 0.01) with TP (0.86), TD (0.88), BL (0.82), HW (0.75), BEA (0.51), COM (0.97), and ANA (0.75). In general, the morphometric variables as a whole demonstrated important correlation coefficients among them (p < 0.01), ranging from 0.38 up to 0.84. To estimate the importance of the morphometric differences between social rank upon estrus induction, a principal component (PC) analysis was performed based on the correlation matrix derived from the corporal measurements. The PC1 and PC2 explained 70.3% and 17.6% of the morphometric variation, respectively. The PC1 was a measure of the goat size (i.e., small, medium, large) and its association with estrus occurrence was evaluated using a logistic regression model; the bigger the goat, the increased probability of being in estrus, by up to five times compared to small goats. Our results confirm that the higher social ranked, larger goats amalgamated some fundamental factors to be successful: aggressiveness, primacy to food access, augmented live weight, and corporal size; all of these were able to modulate out-of-season reproductive success in crossbred dairy goats subjected to an estrus induction protocol and managed under stall-fed conditions in Northern Mexico

Effect of Social Rank upon Estrus Induction and Some Reproductive Outcomes in Anestrus Goats Treated With Progesterone + eCG

We evaluated the possible role of the social rank [R] (i.e., low—LSR, middle—MSR, or high—HSR) in anestrus goats exposed to a P4 + eCG [D] (i.e., 100 or 350 IU) estrus induction protocol (EIP). Adult, multiparous (two to three lactations), multiracial, dairy-type goats (Alpine–Saanen–Nubian x Criollo goats (n = 70; 25°51′ North) managed under stall-fed conditions were all ultrasound evaluated to confirm anestrus status while the R was determined 30 d prior to the EIP. The variables of estrus induction (EI, %), estrus latency (LAT, h), estrus duration (DUR, h), ovulation (OVU, %), ovulation rate (OR, n), corpus luteum size (CLS, cm), pregnancy (PREG, %), kidding (KIDD, %), and litter size (LS, n) as affected by R, D, and the R × D interaction, were evaluated. While OVU and CLS favored (p 0.05; 38.5%) KIDD. However, EI, LAT, DUR, OR, and PREG were affected by the R × D interaction. The HSR group had the largest (p < 0.05) EI % and DUR h, irrespective of D. The shortest (p < 0.05) LAT occurred in D350, irrespective of R. While the largest (p < 0.05) OR occurred in HSR and MSR within D350, the HSR + D350 group had the largest PREG (p < 0.05). These research outcomes are central to defining out-of-season reproductive strategies designed to attenuate seasonal reproduction in goats

Effects of in vivo treatment with the dopamine antagonist pimozide and gonadotropin-releasing hormone agonist (GnRHa) on the reproductive axis of Senegalese sole (Solea senegalensis)

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Histopathology Caused by the Entomopathogenic Fungi, Beauveria bassiana and Metarhizium anisopliae, in the Adult Planthopper, Peregrinus maidis, a Maize Virus Vector

The planthopper Peregrinus maidis (Ashmead) (Hemiptera: Delphacidae) is an important vector of maize viruses in tropical and subtropical areas. Planthoppers are biologically controlled with several species of entomopathogenic fungi that have been isolated from these insect pests of rice in Asia. Beauveria bassiana (Balsamo-Crivelli) Vuillemin and Metarhizium anisopliae (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae) appear to be the most useful against planthoppers because of their ease of mass production, storage, virulence, and application. In the present study, adults of P. maidis infected with B. bassiana and M. anisopliae were observed under light and scanning electron microscopy to characterize morphologically the process of infection and the development of these fungi, prior to and after the death of the host. The hydrophobic conidia of both fungal species were able to attach to all body regions, with a preference for surfaces containing hairs. Few germinated conidia were observed on the insect's body surface at 24, 48, and 72 hr post-inoculation. On the cuticular surface of P. maidis treated with B. bassiana and M. anisopliae, bacillus-like bacteria were observed. These microorganisms could be interacting with fungal conidia, playing a role of antibiosis that will not allow the fungal pathogens to germinate and penetrate. In the colonization events observed in this study, the formation and multiplication of hyphal bodies by both fungal species inside the host's body was noted. The host's whole body was invaded by hyphae between five and six days post-inoculation, and body fat was the most affected tissue

Precision Betacarotene Supplementation Enhanced Ovarian Function and the LH Release Pattern in Yearling Crossbred Anestrous Goats

The possible out-of-season effect of beta-carotene supplementation on ovulation rate (OR), antral follicles (AFN), and total ovarian activity (TOA = OR + AFN) as related to the LH release pattern in yearling anestrous goats was evaluated. In late April, Alpine-Saanen-Nubian x Criollo goats (n = 22, 26 N) were randomly allotted to: (1) Beta-carotene (BETA; n = 10, orally supplemented with 50 mg/goat/d; 36.4 ± 1.07 kg live weight (LW), 3.5 ± 0.20 units, body condition score (BCS) or (2) Non-supplemented (CONT; n = 12, 35.2 ± 1.07 kg LW, 3.4 ± 0.2 units BCS). Upon estrus synchronization, an intensive blood sampling (6 h × 15 min) was accomplished in May for LH quantifications; response variables included (pulsatility-PULSE, time to first pulse-TTFP, amplitude-AMPL, nadir-NAD and area under the curve-AUC). Thereafter, an ultrasonography scanning was completed to assess OR and AFN. The Munro algorithm was used to quantify LH pulsatility; if significant effects of time, treatment or interaction were identified, data were compared across time. Neither LW nor BCS (p > 0.05) or even the LH (p > 0.05); PULSE (4.1 ± 0.9 pulses/6 h), NAD (0.47 ± 0.13 ng) and AUC (51.7 ± 18.6 units) differed between treatments. Nonetheless, OR (1.57 vs. 0.87 ± 0.18 units) and TOA (3.44 vs. 1.87 ± 0.45 units) escorted by a reduced TTFP (33 vs. 126 ± 31.9 min) and an increased AMPL (0.55 vs. 0.24 ± 0.9 ng), favored to the BETA supplemented group (p < 0.05), possibly through a GnRH-LH enhanced pathway and(or) a direct effect at ovarian level. Results are relevant to speed-up the out-of-season reproductive outcomes in goats while may embrace translational applications