Prevalence of the Cryptosporidium Pig Genotype II in Pigs from the Yangtze River Delta, China

Background: Cryptosporidium spp. is prevalent globally, pigs are an important Cryptosporidium reservoir. In China, little data regarding rates of Cryptosporidium infections in pigs are available. The present study was therefore aimed at characterizing the distribution of Cryptosporidium species in pigs from two different cities, Shaoxing and Shanghai, from the Yangtze River delta. Methodology/Principal Findings: Nested PCR to amplify the 18S rRNA locus on DNA extracted from fecal samples (n = 94) revealed the positive rate of Cryptosporidium in pigs from two cities was approximately 17.0%. The positive rates in Shanghai and Shaoxing were 14.3 % and 25.0 % respectively. Amplified sequences were verified by sequencing. The identified strain belonged to the C. pig genotype II using BLAST analysis in the NCBI database. Conclusion/Significance: Our finding of Cryptosporidium pig genotype II in pigs in the Yangtze River delta area suggests that pig farms in this region must be considered a public health threat and proper control measures be introduced

NMR metabolomics reveals effects of Cryptosporidium infections on host cell metabolome

Background: Cryptosporidium is an important gut microbe whose contributions towards infant and immunocompromise patient mortality rates are steadily increasing. Over the last decade, we have seen the development of various tools and methods for studying Cryptosporidium infection and its interactions with their hosts. One area that is sorely overlooked is the effect infection has on host metabolic processes. Results: Using a 1H nuclear magnetic resonance approach to metabolomics, we have explored the nature of the mouse gut metabolome as well as providing the first insight into the metabolome of an infected cell line. Statistical analysis and predictive modelling demonstrated new understandings of the effects of a Cryptosporidium infection, while verifying the presence of known metabolic changes. Of note is the potential contribution of host derived taurine to the diarrhoeal aspects of the disease previously attributed to a solely parasite-based alteration of the gut environment, in addition to other metabolites involved with host cell catabolism. Conclusion: This approach will spearhead our understanding of the Cryptosporidium-host metabolic exchange and provide novel targets for tackling this deadly parasite

Review of Cryptosporidium and Giardia in the eastern part of Europe, 2016

Introduction: This paper reviews the current knowledge and understanding of Cryptosporidium spp. an d Giardia spp. in humans, animals and the environment in 10 countries in the eastern part of Europe: Bosnia and Herzegovina, Croatia, Czech Republic, Estonia, Hungary, Latvia, Poland, Romania, Serbia and Slovenia. Methods: Published scientific papers and conference proceedings from the international and local literature, official national health service reports, national databases and doctoral theses in local languages were reviewed to provide an extensive overview on the epidemiology, diagnostics and research on these pathogens, as well as analyse knowledge gaps and areas for further research. Results: Cryptosporidium spp. and Giardia spp. were found to be common in eastern Europe, but the results from different countries are difficult to compare because of variations in reporting practices and detection methodologies used. Conclusion: Upgrading and making the diagnosis/detection procedures more uniform is recommended throughout the region. Public health authorities should actively work towards increasing reporting and standardising reporting practices as these prerequisites for the reported data to be valid and therefore necessary for appropriate control plans.Peer reviewe

Catalogue of existing good practice examples of programmes and interventions : Deliverable 2.1

This document (D2.1) provides an overview of the extensive data that has been collected on sustainable energy consumption initiatives as part of Work Package 2 (WP2) in ENERGISE. The deliverable provides a general introduction to the scope and objectives of WP2 specifically, as well as a short introduction as to how sustainable energy consumption initiatives are defined in ENERGISE. In addition, a full list is provided of 1000+ sustainable energy consumption initiatives that have been identified throughout Europe

Whistleblowing as a human right: the extent of freedom of speech

V diplomski nalogi sta predstavljena pojma žvižgaštvo in svoboda govora. V prvem delu smo pojma predstavili, v osrednjem delu smo se pregledali pravno ureditev na področju žvižgaštva v Evropi, Sloveniji, Združenih državah Amerike in Rusiji, nalogo smo zaključili z analizo najbolj znanih primerov žvižgačev. Žvižgaštvo in svoboda govora sta pojma, ki sta med sabo zelo povezana, brez njiju pa ne moremo govoriti o sodobnih demokratičnih državah.The thesis presents the concepts of whitleblowing and freedom of speech. In the first part we presented the concept, in the central part we reviewed the legislation in the field of whistleblowing in Europe, Slovenia, the United States and Russia, we then finished the thesis with an analysis of the most known examples of whistleblowers. Whistleblowing and freedom of speach are two very connected concepts, without them we cannot speak of modern democracies

The impact of information technology on mcdonald\u27s business

V mojem diplomskem delu sem predstavil kaj je informacijska tehnologija, čemu služi in kako jo podjetja uporabljajo. Pisal sem tudi o vplivu informacijske tehnologije na podjetja, kjer sem se osredotočil na poslovanje, marketing in analizo podatkov. Nato sem še predstavil informacijsko tehnologijo na primeru McDonalds, kako ta tehnologija vpliva na gostoljubnost in storitev franšize, kako mcdonalds uporablja informacijsko tehnologijo v svojem vsakodnevnem poslovanju in povezavo med informacijsko tehnologijo in marketingom. Omenil sem tudi tehnologije, katere je McDonalds implementiral, ki so pomagale pri izboljšavi poslovanja podjetja McDonalds in približevanju svojih izdelkov in storitve svojim strankam. Za na konec pa sem se odločil, da se bom poglobil v aplikacijo »McDonalds«, ki se je izkazala kot zelo velik uspeh in posledično spremenila interakcijo med podjetjem in potrošnikom.In my diploma, I presented what information technology is, what it serves and how companys use it. I also wrote about the impact of information technology on companys, where i focused on business, marketing and data analysis. I then presented information technology on the case of McDonalds, how that technology effects the hospitality and service of the franchise, how McDonalds uses information technology in its daily business and the connection between information technology and marketing. I also mentioned the technologies implemented by McDonalds that have helped improve McDonalds business and bring their products and services closer to their costumers. In the end, I decided to delve in to the »McDonalds« application, which proved to be a great success and consequently changed the interaction between the company and the consumers

Latent microsporidiosis caused by Encephalitozoon cuniculi in immunocompetent hosts: a murine model demonstrating the ineffectiveness of the immune system and treatment with albendazole.

BACKGROUND: Microsporidia are obligate intracellular parasites causing severe infections with lethal outcome in immunocompromised hosts. However, these pathogens are more frequently reported as latent infections in immunocompetent individuals and raises questions about the potential risk of reactivation following induced immunosuppression. AIMS: To evaluate the possibility latent microsporidiosis, efficacy or albendazole, and reactivation, the authors monitored the course of E. cuniculi infection in immunocompetent BALB/c mice and immunodeficient SCID mice using molecular methods. METHODS: Mice were per orally infected with 10(7) spores of E. cuniculi. Selected groups were treated with albendazole, re-infected or chemically immunosuppressed by dexamethasone. The presence of microsporidia in the host's organs and feces were determined using PCR methods. Changes in numbers of lymphocytes in blood and in spleen after induction of immunosuppression were confirmed using flow cytometry analysis. RESULTS: Whereas E. cuniculi caused lethal microsporidiosis in SCID mice, the infection in BABL/c mice remained asymptomatic despite parasite dissemination into many organs during the acute infection phase. Albendazole treatment led to microsporidia elimination from organs in BALB/c mice. In SCID mice, however, only a temporary reduction in number of affected organs was observed and infection re-established post-treatment. Dexamethasone treatment resulted in a chronic microsporidia infection disseminating into most organs in BALB/c mice. Although the presence of E. cuniculi in organs of albendazole- treated mice was undetectable by PCR, it was striking that infection was reactivated by immunosuppression treatment. CONCLUSION: Our results demonstrated that microsporidia can successfully survive in organs of immunocompetent hosts and are able to reactivate from undetectable levels and spread within these hosts after induction of immunosuppression. These findings stress the danger of latent microsporidiosis as a life-threatening risk factor especially for individuals undergoing chemotherapy and in transplant recipients of organs originating from infected donors

Genetic characterizations of Cryptosporidium spp. from pet rodents indicate high zoonotic potential of pathogens from chinchillas

Cryptosporidium spp. are common protozoan pathogens in mammals. With pet rodents being integrated into modern life, the potential roles of them in transmitting parasites to humans need assessments. In the present study, we examined the occurrence of Cryptosporidium spp. in pet rodents in Guangdong, south China. A total of 697 fecal samples were collected from 11 species of rodents in seven pet shops, one pet market and one farm. Cryptosporidium spp. were identified by PCR analysis of the small subunit rRNA gene. An overall infection rate of 36.9% (257/697) was obtained, with infection rates varying from 9.3% in chinchillas, 52.3% in guinea pigs, 57.1% in squirrels, to 68.4% in cricetid animals. Nine Cryptosporidium species and genotypes were identified, including C. wrairi (in 129 guinea pigs), C. andersoni (in 34 hamsters), C. homai (in 32 guinea pigs), Cryptosporidium hamster genotype (in 30 hamsters), C. ubiquitum (in 24 chinchillas and squirrels), C. parvum (in 2 chinchillas), Cryptosporidium ferret genotype (in 2 chipmunks), C. muris (in 1 hamster and 1 guinea pig), and Cryptosporidium chipmunk genotype V (in 1 chinchilla and 1 chipmunk). Sequence analysis of the 60 kDa glycoprotein gene identified three subtype families of C. ubiquitum, including family XIId in 15 chinchillas, XIIa in 5 chinchillas, and a new subtype family (XIIi) in 1 squirrel. The identification of C. parvum and C. ubiquitum in pet rodents suggests that these animals, especially chinchillas, could serve as reservoirs of human-pathogenic Cryptosporidium spp. Hygiene should be practiced in the rear and care of these animals, and One Health measures should be developed to reduce the occurrence of zoonotic Cryptosporidium infections due to contact with pet rodents