Direct on-chip differentiation of intestinal tubules from induced pluripotent stem cells

Intestinal organoids have emerged as the new paradigm for modelling the healthy and diseased intestine with patient-relevant properties. In this study, we show directed differentiation of induced pluripotent stem cells towards intestinal-like phenotype within a microfluidic device. iPSCs are cultured against a gel in microfluidic chips of the OrganoPlate, in which they undergo stepwise differentiation. Cells form a tubular structure, lose their stem cell markers and start expressing mature intestinal markers, including markers for Paneth cells, enterocytes and neuroendocrine cells. Tubes develop barrier properties as confirmed by transepithelial electrical resistance (TEER). Lastly, we show that tubules respond to pro-inflammatory cytokine triggers. The whole procedure for differentiation lasts 14 days, making it an efficient process to make patient-specific organoid tubules. We anticipate the usage of the platform for disease modelling and drug candidate screening

Lorentz Invariance Violation induced time delays in GRBs in different cosmological models

Lorentz Invariance Violation (LIV) manifesting itself by energy dependent modification of standard relativistic dispersion relation has recently attracted a considerable attention. Ellis et al. previously investigated the energy dependent time offsets in different energy bands on a sample of gamma ray bursts and, assuming standard cosmological model, they found a weak indication for redshift dependence of time delays suggestive of LIV. Going beyond the $\Lambda$CDM cosmology we extend this analysis considering also four alternative models of dark energy (quintessence with constant and variable equation of state, Chaplygin gas and brane-world cosmology). It turns out that the effect noticed by Ellis et al. is also present in those models and is the strongest for quintessence with variable equation of state.Comment: 14 pages, 1 figur

Density growth in Kantowski-Sachs cosmologies with cosmological constant

In this work the growth of density perturbations in Kantowski-Sachs cosmologies with a positive cosmological constant is studied, using the 1+3 and 1+1+2 covariant formalisms. For each wave number we obtain a closed system for scalars formed from quantities that are zero on the background and hence are gauge-invariant. The solutions to this system are then analyzed both analytically and numerically. In particular the effects of anisotropy and the behaviour close to a bounce in the cosmic scale factor are considered. We find that typically the density gradient in the bouncing directions experiences a local maximum at or slightly after the bounce.Comment: 33 pages, 17 picture

Reconstructing the properties of dark energy from recent observations

We explore the properties of dark energy from recent observational data, including the Gold Sne Ia, the baryonic acoustic oscillation peak from SDSS, the CMB shift parameter from WMAP3, the X-ray gas mass fraction in cluster and the Hubble parameter versus redshift. The $\Lambda CDM$ model with curvature and two parameterized dark energy models are studied. For the $\Lambda CDM$ model, we find that the flat universe is consistent with observations at the $1\sigma$ confidence level and a closed universe is slightly favored by these data. For two parameterized dark energy models, with the prior given on the present matter density, $\Omega_{m0}$, with $\Omega_{m0}=0.24$, $\Omega_{m0}=0.28$ and $\Omega_{m0}=0.32$, our result seems to suggest that the trend of $\Omega_{m0}$ dependence for an evolving dark energy from a combination of the observational data sets is model-dependent.Comment: 16 pages, 15 figures, To appear in JCA

The effect of inhomogeneities on the distance to the last scattering surface and the accuracy of the CMB analysis

The standard analysis of the CMB data assumes that the distance to the last scattering surface can be calculated using the distance-redshift relation as in the Friedmann model. However, in the inhomogeneous universe, even if =0, the distance relation is not the same as in the unperturbed universe. This can be of serious consequences as a change of distance affects the mapping of CMB temperature fluctuations into the angular power spectrum. In addition, if the change of distance is relatively uniform no new temperature fluctuations are generated. It is therefore a different effect than the lensing or ISW effects which introduce additional CMB anisotropies. This paper shows that the accuracy of the CMB analysis can be impaired by the accuracy of calculation of the distance within the cosmological models. Since this effect has not been fully explored before, to test how the inhomogeneities affect the distance-redshift relation, several methods are examined: the Dyer-Roeder relation, lensing approximation, and non-linear Swiss-Cheese model. In all cases, the distance to the last scattering surface is different than when homogeneity is assumed. The difference can be as low as 1% and as high as 80%. Excluding extreme cases, the distance changes by about 20-30%. Since the distance to the last scattering surface is set by the position of the CMB peaks, in order to have a good fit, the distance needs to be adjusted. After correcting the distance, the cosmological parameters change. Therefore, a not properly estimated distance to the last scattering surface can be a major source of systematics. This paper shows that if inhomogeneities are taken into account when calculating the distance then models with positive spatial curvature and with \Omega_\Lambda ~ 0.8-0.9 are preferred. The \Lambda CDM model in most cases, is at odds with the current data.Comment: 18 pages, 6 figure

Interplay among transversity induced asymmetries in hadron leptoproduction

In the fragmentation of a transversely polarized quark several left-right asymmetries are possible for the hadrons in the jet. When only one unpolarized hadron is selected, it exhibits an azimuthal modulation known as Collins effect. When a pair of oppositely charged hadrons is observed, three asymmetries can be considered, a di-hadron asymmetry and two single hadron asymmetries. In lepton deep inelastic scattering on transversely polarized nucleons all these asymmetries are coupled with the transversity distribution. From the high statistics COMPASS data on oppositely charged hadron-pair production we have investigated for the first time the dependence of these three asymmetries on the difference of the azimuthal angles of the two hadrons. The similarity of transversity induced single and di-hadron asymmetries is discussed. A new analysis of the data allows to establish quantitative relationships among them, providing for the first time strong experimental indication that the underlying fragmentation mechanisms are all driven by a common physical process.Comment: 6 figure

Interplay among transversity induced asymmetries in hadron leptoproduction

In the fragmentation of a transversely polarized quark several left-right asymmetries are possible for the hadrons in the jet. When only one unpolarized hadron is selected, it exhibits an azimuthal modulation known as Collins effect. When a pair of oppositely charged hadrons is observed, three asymmetries can be considered, a di-hadron asymmetry and two single hadron asymmetries. In lepton deep inelastic scattering on transversely polarized nucleons all these asymmetries are coupled with the transversity distribution. From the high statistics COMPASS data on oppositely charged hadron-pair production we have investigated for the first time the dependence of these three asymmetries on the difference of the azimuthal angles of the two hadrons. The similarity of transversity induced single and di-hadron asymmetries is discussed. A new analysis of the data allows to establish quantitative relationships among them, providing for the first time strong experimental indication that the underlying fragmentation mechanisms are all driven by a common physical process.Comment: 6 figure

Longitudinal double spin asymmetries in single hadron quasi-real photoproduction at high pTp_T

We measured the longitudinal double spin asymmetries $A_{LL}$ for single hadron muo-production off protons and deuterons at photon virtuality $Q^2$ < 1(GeV/$\it c$)$^2$ for transverse hadron momenta $p_T$ in the range 0.7 GeV/$\it c$ to 4 GeV/$\it c$ . They were determined using COMPASS data taken with a polarised muon beam of 160 GeV/$\it c$ or 200 GeV/$\it c$ impinging on polarised $\mathrm{{}^6LiD}$ or $\mathrm{NH_3}$ targets. The experimental asymmetries are compared to next-to-leading order pQCD calculations, and are sensitive to the gluon polarisation $\Delta G$ inside the nucleon in the range of the nucleon momentum fraction carried by gluons $0.05 < x_g < 0.2$

Conservative treatment of a left atrial intramural hematoma after left atrial thrombus resection and concomitant mitral valve replacement - case report

Left atrial intramural hematoma is a seldom cause of left atrial mass. It has been described to occur spontaneously, after interventional procedures, after blunt chest trauma, or after aortocoronary bypass surgery. We present a case of mitral valve replacement together with the removal of a large intraatrial space-occupying lesion. Intraoperative transesophageal echocardiography confirmed a successful resection of this mass. Surprisingly, upon admission to ICU, transesophageal and transthoracic echocardiography revealed a recurrence of an intramural lesion, closest matching a hematoma, which was confirmed by contrast-enhanced computed tomography. Surgical intervention was thoroughly discussed but a conservative management was favoured. 3 months after surgery, a reassessed transthoracic echocardiography and computed tomography demonstrated an almost complete resolution of the pre-existing hematoma