Effects of different ceramic and dentin thicknesses on the temperature rise during photocuring

AbstractBackground/purposeThe aims of this investigation were to describe the effect of different ceramic and remaining dentin thicknesses on substrate temperature during photocuring, and investigate whether the temperature increased by >5.5°C for different dentin/ceramic combinations.Materials and methodsThree groups of dentin thicknesses of 1.0 (D1.0), 1.5 (D1.5), and 2.0 mm (D2.0), and three groups of ceramic thicknesses of 1.5 (C1.5), 2.5 (C2.5), and 3.5 mm (C3.5) were examined. Temperature changes and the maximum temperature were observed under a high-intensity halogen light (QTH-Atralis 10 ECS program at 1200mW/cm2 for 30 seconds, Ivoclar Vivadent AG, Schaan, Liechtenstein). Four groups, D1.0–C1.5 (+11°C), D1.5–C1.5 (+7.2°C), D1.0–C2.5 (+6.7°C), and D2–0C1.5 (+5.8°C), demonstrated temperature changes of >5.5°C.Results and ConclusionsA statistical analysis showed that separate individual thicknesses and combinations of dentin and ceramic had significant effects on temperature changes (P<0.01). It was observed that the ceramic exhibited a smaller temperature shielding effect than dentin. Clinically, it would be optimal to preserve the dentin to avoid damaging pulp tissues. Where there is insufficient overall thickness (≤3.5mm), continuous high-energy output photocuring should be avoided to protect pulp tissues from thermal injury

Comparison of breast motion at different levels of support during physical activity

Optical tracking systems have been used in previous studies to capture the motion of the nude breast and breasts in bras, under the assumption that no breast-bra relative movement occurred within the bra. This study compared breast and bra movement through electromagnetic tracking and optical tracking systems to determine the relative breast movement occurring with different breast support and exercise-induced breast discomfort. Total of 30 female participants (mean age: 21.5 ± 2.3 years; cup sizes: A-F) were recruited and their movement at four different levels of breast support was recorded in two motion capture systems for further analysis and comparison. Significant differences between bra and breast vertical displacement were found at all support levels during periods of intense movement (r = 0.556; p < 0.05). Because the greatest bra displacement was observed when participants wore an everyday bra and the greatest breast displacement was observed when participants wore a crop-top bra, there was evident inconsistency in bra and breast motion and a high-impact sports bra was the most effective to reduce breast movement and discomfort among the four types of bras. An electromagnetic tracking system provided direct observation of the actual movement of the breasts, and an optical tracking system enabled us to monitor bra displacement. Significant differences were observed in bra and breast displacement during the intense movements included in this study. The results bring into question the assumption made in previous studies that no relative movement occurs within a breast support garment.This study is supported by Ministry of Science and Technology grants 103-2815-C-006-037-H and 105-2410-H-006-052, TAIWAN, and OT Motion Lab, Department of Occupational Therapy, National Cheng Kung University

Cloning, purification, and functional characterization of Carocin S2, a ribonuclease bacteriocin produced by Pectobacterium carotovorum

<p>Abstract</p> <p>Background</p> <p>Most isolates of <it>Pectobacterium carotovorum subsp. carotovorum </it>(Pcc) produce bacteriocins. In this study, we have determined that Pcc strain F-rif-18 has a chromosomal gene encoding the low-molecular-weight bacteriocin, Carocin S2, and that this bacteriocin inhibits the growth of a closely related strain. Carocin S2 is inducible by ultraviolet radiation but not by mutagenic agents such as mitomycin C.</p> <p>Results</p> <p>A carocin S2-defective mutant, TF1-2, was obtained by Tn5 insertional mutagenesis using F-rif-18. A 5706-bp DNA fragment was detected by Southern blotting, selected from a genomic DNA library, and cloned to the vector, pMS2KI. Two adjacent complete open reading frames within pMS2KI were sequenced, characterized, and identified as caroS2K and caroS2I, which respectively encode the killing protein and immunity protein. Notably, carocin S2 could be expressed not only in the mutant TF1-2 but also in <it>Escherichia coli </it>DH5α after entry of the plasmid pMS2KI. Furthermore, the C-terminal domain of CaroS2K was homologous to the nuclease domains of colicin D and klebicin D. Moreover, SDS-PAGE analysis showed that the relative mass of CaroS2K was 85 kDa and that of CaroS2I was 10 kDa.</p> <p>Conclusion</p> <p>This study shown that another nuclease type of bacteriocin was found in <it>Pectobacterium carotovorum</it>. This new type of bacteriocin, Carocin S2, has the ribonuclease activity of CaroS2K and the immunity protein activity of CaroS2I.</p

An overview of epigenetics and chemoprevention

AbstractIt is now appreciated that both genetic alteration, e.g. mutations, and aberrant epigenetic changes, e.g. DNA methylation, cause cancer. Epigenetic dysregulation is potentially reversible which makes it attractive as targets for cancer prevention. Synthetic drugs targeting enzymes, e.g. DNA methyltransferase and histone deacetylase, that regulate epigenetic patterns are active in clinical settings. In addition, dietary factors have been suggested to have potential to reverse aberrant epigenetic patterns. Uncovering the human epigenome can lead us to better understand the dynamics of DNA methylation in disease progression which can further assist in cancer prevention

The glucocorticoid-Angptl4-ceramide axis induces insulin resistance through PP2A and PKCζ.

Chronic glucocorticoid exposure is associated with the development of insulin resistance. We showed that glucocorticoid-induced insulin resistance was attenuated upon ablation of Angptl4, a glucocorticoid target gene encoding the secreted protein angiopoietin-like 4, which mediates glucocorticoid-induced lipolysis in white adipose tissue. Through metabolomic profiling, we revealed that glucocorticoid treatment increased hepatic ceramide concentrations by inducing enzymes in the ceramide synthetic pathway in an Angptl4-dependent manner. Angptl4 was also required for glucocorticoids to stimulate the activities of the downstream effectors of ceramide, protein phosphatase 2A (PP2A) and protein kinase Cζ (PKCζ). We further showed that knockdown of PP2A or inhibition of PKCζ or ceramide synthesis prevented glucocorticoid-induced glucose intolerance in wild-type mice. Moreover, the inhibition of PKCζ or ceramide synthesis did not further improve glucose tolerance in Angptl4-/- mice, suggesting that these molecules were major downstream effectors of Angptl4. Overall, our study demonstrates the key role of Angptl4 in glucocorticoid-augmented hepatic ceramide production that induces whole-body insulin resistance

Comparison of breast motion at different levels of support during physical activity

Optical tracking systems have been used in previous studies to capture the motion of the nude breast and breasts in bras, under the assumption that no breast-bra relative movement occurred within the bra. This study compared breast and bra movement through electromagnetic tracking and optical tracking systems to determine the relative breast movement occurring with different breast support and exercise-induced breast discomfort. Total of 30 female participants (mean age: 21.5 ± 2.3 years; cup sizes: A-F) were recruited and their movement at four different levels of breast support was recorded in two motion capture systems for further analysis and comparison. Significant differences between bra and breast vertical displacement were found at all support levels during periods of intense movement (r = 0.556; p < 0.05). Because the greatest bra displacement was observed when participants wore an everyday bra and the greatest breast displacement was observed when participants wore a crop-top bra, there was evident inconsistency in bra and breast motion and a high-impact sports bra was the most effective to reduce breast movement and discomfort among the four types of bras. An electromagnetic tracking system provided direct observation of the actual movement of the breasts, and an optical tracking system enabled us to monitor bra displacement. Significant differences were observed in bra and breast displacement during the intense movements included in this study. The results bring into question the assumption made in previous studies that no relative movement occurs within a breast support garment

Does Long-Term Use of Silver Nanoparticles Have Persistent Inhibitory Effect on H. pylori Based on Mongolian Gerbil’s Model?

It is urgent to find alternative agents due to increasing failure rate of Helicobacter pylori (H. pylori) eradication. The study surveyed the long-term effect of silver nanoparticles (AgNP) on H. pylori based on Mongolian gerbil's model

Self-supervised learning-based general laboratory progress pretrained model for cardiovascular event detection

The inherent nature of patient data poses several challenges. Prevalent cases amass substantial longitudinal data owing to their patient volume and consistent follow-ups, however, longitudinal laboratory data are renowned for their irregularity, temporality, absenteeism, and sparsity; In contrast, recruitment for rare or specific cases is often constrained due to their limited patient size and episodic observations. This study employed self-supervised learning (SSL) to pretrain a generalized laboratory progress (GLP) model that captures the overall progression of six common laboratory markers in prevalent cardiovascular cases, with the intention of transferring this knowledge to aid in the detection of specific cardiovascular event. GLP implemented a two-stage training approach, leveraging the information embedded within interpolated data and amplify the performance of SSL. After GLP pretraining, it is transferred for TVR detection. The proposed two-stage training improved the performance of pure SSL, and the transferability of GLP exhibited distinctiveness. After GLP processing, the classification exhibited a notable enhancement, with averaged accuracy rising from 0.63 to 0.90. All evaluated metrics demonstrated substantial superiority (p < 0.01) compared to prior GLP processing. Our study effectively engages in translational engineering by transferring patient progression of cardiovascular laboratory parameters from one patient group to another, transcending the limitations of data availability. The transferability of disease progression optimized the strategies of examinations and treatments, and improves patient prognosis while using commonly available laboratory parameters. The potential for expanding this approach to encompass other diseases holds great promise.Comment: published in IEEE Journal of Translational Engineering in Health & Medicin