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Oratie uitgesproken door Prof.dr. E.J. Kuijper bij de aanvaarding van het ambt van hoogleraar in de Experimentele Bacteriologieaan de Universiteit Leiden op vrijdag 20 december 201

Inaccuracy of routine susceptibility tests for detection of erythromycin resistance of Campylobacter jejuni and Campylobacter coli

In The Netherlands, both an increase in and regional differences in erythromycin resistance of Campylobacter jejuni and Campylobacter coli have been reported. To determine the accuracy of routine tests for erythromycin resistance, 48 erythromycin-resistant isolates from various laboratories that participate in the Dutch surveillance of Campylobacter infections were reinvestigated. Initial susceptibility testing for erythromycin had been performed by disk diffusion in six and MIC-based methods in two laboratories. Reinvestigation was carried out using broth microdilution as a reference standard, as well as E-test and genetic resistance testing. Of 36 C. jejuni isolates reported by the initial laboratories as erythromycin-resistant, four (11%) and five (14%) were confirmed as erythromycin-resistant using broth microdilution according to CLSI and EUCAST resistance criteria, respectively. Erythromycin resistance was found in eight of 12 (67%) C. coli isolates according to both criteria. Results of E-tests were in accordance with these results in all isolates. Resistance-associated mutations in the 23S rRNA gene (A2059G and A2058T) were found in all isolates showing high-level resistance, whereas none were found in susceptible isolates. Routine determination of the erythromycin resistance of C. jejuni and C. coli shows unacceptable interlaboratory variation. In the absence of standardized protocols and interpretive criteria for disk diffusion, and while we await the development of easily applicable and reliable methods for molecular resistance testing, the use of broth microdilution remains the best method

Recovery of Mycobacterium haemophilum skin infection in an HIV-I-infected patient after the start of antiretroviral triple therapy

Constant Rate Thermal Analysis (CRTA) method implies controlling the temperature in such a way that the reaction rate is maintained constant all over the process. This method allows determining simultaneously both the kinetic parameters and the kinetic model from a single experiment as the shape of the CRTA α-T curves strongly depends on the kinetic model. CRTA method has been developed in the market only for thermogravimetric and thermodilatometric systems and, therefore, its use has been limited until now to the kinetic study of processes involving changes in mass or size of the samples, respectively. To overcome this obstacle, a method has been developed in this work for using the DSC signal for controlling the process rate in such a way that CRTA would be applied to the kinetic analysis of either phase transformations or crystallizations. The advantages of CRTA for performing the kinetics of crystallization processes have been here successfully demonstrated for the first time after selecting the crystallization of zirconia gel as test reaction.Ministerio de Economía y Competitividad CTQ2014-52763-C2-1-RJunta de Andalucía TEP-7858, TEP-1900FEDER CTQ2014-52763-C2-1-RFEDER TEP-7858 TEP-190

Висвітлення діяльності опозиційного руху в УРСР у ІІ пол. 1960-х – поч. 1980-х рр. у наукових дослідженнях

В статті досліджуються основні історіографічні тенденції вивчення діяльності опозиційного руху в УРСР в другій половині 1960-х – початку 1980-х рр.В статье исследуются основные историографические тенденции изучения оппозиционного движения в УССР во второй половине 1960-х – начале 1980-х гг.The article is dedicated to the main historiographical trends in the investigations of oppositional movements in Soviet Ukraine during second half of 1960th – at the beginning 1980th

Faecal microbiota replacement to eradicate antimicrobial resistant bacteria in the intestinal tract - a systematic review

Purpose of review Antimicrobial resistance is a rising threat to global health and is associated with increased mortality. Intestinal colonisation with multidrug-resistant organisms (MDRO) can precede invasive infection and facilitates spread within communities and hospitals. Novel decolonisation strategies, such as faecal microbiota transplantation (FMT), are being explored. The purpose of this review is to provide an update on how the field of FMT for MDRO decolonisation has developed during the past year and to assess the efficacy of FMT for intestinal MDRO decolonisation. Recent findings Since 2020, seven highly heterogenous, small, nonrandomised cohort studies and five case reports have been published. In line with previous literature, decolonisation rates ranged from 20 to 90% between studies and were slightly higher for carbapenem-resistant Enterobacteriaceae than vancomycin-resistant Enterococcus. Despite moderate decolonisation rates in two studies, a reduction in MDRO bloodstream and urinary tract infections was observed. Summary and implications Although a number of smaller cohort studies show some effect of FMT for MDRO decolonisation, questions remain regarding the true efficacy of FMT (taking spontaneous decolonisation into account), the optimal route of administration, the role of antibiotics pre and post-FMT and the efficacy in different patient populations. The observed decrease in MDRO infections post-FMT warrants further research.Immunogenetics and cellular immunology of bacterial infectious disease

DNA replication proteins as potential targets for antimicrobials in drug-resistant bacterial pathogens

Molecular basis of bacterial pathogenesis, virulence factors and antibiotic resistanc

Effect on diagnostic yield of repeated stool testing during outbreaks of Clostridium difficile-associated disease

ABSTRACTThe effect on diagnostic yield of testing sequential stools was assessed during two hospital epidemics of Clostridium difficile. Using a rapid immunoassay, C. difficile-associated disease was diagnosed in 237 diarrhoeal patients, of whom 204 (86%) were diagnosed from the first faeces sample and 12 (5%) were diagnosed from follow-up samples obtained within 1 week. The remaining 21 (9%) patients yielded a positive test from stools obtained >1 week after the initial negative sample. It was concluded that repeated testing of stools for C. difficile toxin is of value in controlling outbreaks of C. difficile infection