Supporting Information II for: "New Steroidal 4-Aminoquinolines Antagonize Botulinum Neurotoxin Serotype A in Mouse Embryonic Stem Cell Derived Motor Neurons in Postintoxication Model"

NMR spectra and HPLC purity spectra of all tested compoundsSupporting information II for: Konstantinović, J. M., Kiris, E., Kota, K. P., Kugelman-Tonos, J., Videnović, M., Cazares, L. H., Terzić-Jovanović, N., Verbić, T., Anđelković, B. D., Duplantier, A. J., Bavari, S.,& Šolaja, B. (2018). New Steroidal 4-Aminoquinolines Antagonize Botulinum Neurotoxin Serotype A in Mouse Embryonic Stem Cell Derived Motor Neurons in Postintoxication Model. Journal of Medicinal Chemistry, American Chemical Society (ACS)., 61(4), 1595-1608. [https://doi.org/10.1021/acs.jmedchem.7b01710]The published version of the article: [https://cer.ihtm.bg.ac.rs/handle/123456789/2325]The peer-reviewed version of the article: [http://cer.ihtm.bg.ac.rs/handle/123456789/2935]Additional supporting information: [https://cer.ihtm.bg.ac.rs/handle/123456789/4515]Molecular formula strings and additional data: [https://cer.ihtm.bg.ac.rs/handle/123456789/4517

TELEX HEBDOMADAIRE NR 186 DU 12 OCTOBRE 1984 ADRESSE A L'ENSEMBLE DES DELEGATIONS EXTERIEURES ET BUREAUX DE PRESS ET D'INFORMATION INDEPENDANTS DANS LES PAYS TIERS = WEEKLY MEMO NO. 186 ON OCTOBER 12, 1984 TO FOREIGN DELEGATIONS AND PRESS BUREAUS OF THIRD COUNTRIES

Inhibitory activities against BoNT/A LC and holotoxin in proteolytic and cell-based assay for all tested compounds; fluorescence and UV–vis spectra for determination of 16 binding to HSA and AGP; ligand interaction diagrams, docking scores, and docking–in vitro inhibitory activity correlations; spectral and analytical data for all synthesized compounds; detailed procedures for the determination of the HPLC purity.Supporting information I for: Konstantinović, J. M., Kiris, E., Kota, K. P., Kugelman-Tonos, J., Videnović, M., Cazares, L. H., Terzić-Jovanović, N., Verbić, T., Anđelković, B. D., Duplantier, A. J., Bavari, S.,& Šolaja, B. (2018). New Steroidal 4-Aminoquinolines Antagonize Botulinum Neurotoxin Serotype A in Mouse Embryonic Stem Cell Derived Motor Neurons in Postintoxication Model. Journal of Medicinal Chemistry, American Chemical Society (ACS)., 61(4), 1595-1608. [https://doi.org/10.1021/acs.jmedchem.7b01710]The published version of the article: [https://cer.ihtm.bg.ac.rs/handle/123456789/2325]The peer-reviewed version of the article: [http://cer.ihtm.bg.ac.rs/handle/123456789/2935]Additional supporting information (NMR spectra and HPLC purity spectra of all tested compounds): [https://cer.ihtm.bg.ac.rs/handle/123456789/4516]Molecular formula strings and additional data: [https://cer.ihtm.bg.ac.rs/handle/123456789/4517

Supplementary data for the article: Konstantinović, J.; Kiris, E.; Kota, K. P.; Kugelman-Tonos, J.; Videnović, M.; Cazares, L. H.; Terzić Jovanović, N.; Verbić, T. Ž.; Andjelković, B.; Duplantier, A. J.; et al. New Steroidal 4-Aminoquinolines Antagonize Botulinum Neurotoxin Serotype A in Mouse Embryonic Stem Cell Derived Motor Neurons in Postintoxication Model. Journal of Medicinal Chemistry 2018, 61 (4), 1595–1608. https://doi.org/10.1021/acs.jmedchem.7b01710

Supplementary material for: [https://doi.org/10.1021/acs.jmedchem.7b01710]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2099

Emergence of Ebola Virus Escape Variants in Infected Nonhuman Primates Treated with the MB-003 Antibody Cocktail

MB-003, a plant-derived monoclonal antibody cocktail used effectively in treatment of Ebola virus infection in non-human primates, was unable to protect two of six animals when initiated 1 or 2 days post-infection. We characterized a mechanism of viral escape in one of the animals, after observation of two clusters of genomic mutations that resulted in five nonsynonymous mutations in the monoclonal antibody target sites. These mutations were linked to a reduction in antibody binding and later confirmed to be present in a viral isolate that was not neutralized in vitro. Retrospective evaluation of a second independent study allowed the identification of a similar case. Four SNPs in previously identified positions were found in this second fatality, suggesting that genetic drift could be a potential cause for treatment failure. These findings highlight the importance selecting different target domains for each component of the cocktail to minimize the potential for viral escape