Developing a Computer Model and Its Application in Evaluating Forward Cleaner Sequences

The objection of this work was to establish a useable format for developing an idea into a working model and to verify that format by example. The format is outlined as follows: Establish a need Set Objectives/Criteria Synthesis Analysis Selection Decision/Action Communication The example used to verify the format was a comparison of various forward cleaner sequences to determine if an improvement could be made over the current cascade system. After completion of the model format, it was determined that an alpha/alpha cleaning system would prove more cost effective than a cascade system if it was run at feed consistencies greater than .55%. The incremental cleaning efficiency would allow for lower cost stocks to be used, thus defraying the increased capital costs. The overall application of the model format proved very effective

Segmental isotopic labeling by asparaginyl endopeptidase-mediated protein ligation

Segmental isotopic labeling can facilitate NMR studies of large proteins, multi-domain proteins, and proteins with repetitive sequences by alleviating NMR signal overlaps. Segmental isotopic labeling also allows us to investigate an individual domain in the context of a full-length protein by NMR. Several established methods are available for segmental isotopic labeling such as intein-mediated ligation, but each has specific requirements and limitations. Here, we report an enzymatic approach using bacterially produced asparagine endopeptidase from Oldenlandia affinis for segmental isotopic labeling of a protein with repetitive sequences, a designed armadillo repeat protein, by overcoming some of the shortcomings of enzymatic ligation for segmental isotopic labeling.Peer reviewe

2019 Scholars at Work Conference Program

Program for the 2019 Scholars at Work Conference at Minnesota State University, Mankato on March 29, 2019

NPM Reforms in Napoleonic Countries: A Comparative Study of Management Accounting Innovations in Greek and Italian Municipalities

his is a comparative study of the rationale and effects of management accounting innovations in Greek and Italian municipalities, as part of the NPM reform in the public sector. This article aims to understand the extent of adoption of accounting innovations in Greek and Italian municipalities, and the factors affecting the specific techniques in use. The study reports on a survey based on medium-sized and large Italian and Greek municipalities, targeted at public municipalities’ accountants, the users of accounting techniques. The study identifies the intended purposes of NPM reform and, particularly, management accounting innovations, analyzes a set of factors that affect the implementation of management accounting technologies, and discusses determinants and features of such innovation on a comparative perspective