Mikrobiološka transformacija sintetičkog laktona benzofuranskog tipa

The aim of this work was the investigation of the biotransformation ability of a synthetic benzofurane type g-lactone. This workwas basedon the microbiological reactions of Aspergillus niger, Saccharomyces cerevisiae, Bacillus mycoides, Agrobacterium tumefa- ciens,Pseudomonas glicinea and Pseudomonas fluorescens to obtain newactive derivatives. Four biotransformation products were determined. The lactone was used for two purposes, as an additive for the nutrient substrate and as a source of organic carbon.Cilj ovog rada je ispitivanje mogućnosti biotransformacije benzofuranskog derivata, sa a-metilen-g-butirolaktonskom strukturnom jedinicom, sa različitim sojevima mikroorganizama: Aspergillus niger, Saccharomyces cerrevisiae, Bacillus mycoides, Agrobacterium tumefaciens, Pseudomonas glicinea i Pseudomonas fluorescens u cilju dobivanja novih jedinjenja sa specifičnijom antimikrobnom aktivnošću. Takodje izolovani i identifikovani proizvodi su ukazali i potvrdili osnovne biodegradacione puteve ispitivanog laktona

Thermal noise in aqueous quadrupole micro- and nano-traps

Recent simulations and experiments with aqueous quadrupole micro-traps have confirmed a possibility for control and localization of motion of a charged particle in a water environment, also predicting a possibility of further reduction of the trap size to tens of nano-meters for trapping charged bio-molecules and DNA segments. We study the random thermal noise due to Brownian motion in water which significantly influences the trapping of particles in an aqueous environment. We derive the exact, closed-form expressions for the thermal fluctuations of position and velocity of a trapped particle and thoroughly examine the properties of the rms for the fluctuations as functions of the system parameters and time. The instantaneous signal transferring mechanism between the velocity and position fluctuations could not be achieved in the previous phase-average approaches

Примена пракси амбијенталних уметности у архитектонском пројектовању простора свакодневнице

From a theoretical standpoint, this is an interdisciplinary doctoral thesis, combining contemporary design practices with their phenomenological interpretation using the notion of atmosphere of a space. The theme of the dissertation is a definition of the concept of architectural atmosphere, its theoretical interpretation through research of environmental art practice and its analysis through the study of everyday spaces in the designs of architect Vasilije Milunović. The main research objective is to establish a practical relationship between the practices of environmental art and architectural design, in order to open architectural design practice towards critical and research potentials of interdisciplinary and hybrid practices in artistic creation. The usefulness of the established relations is reflected in the possibility of interdisciplinary exchange of research tactics and techniques, as well as through the possibility of improvement of design practice through broadening of the area and of the media of research and work. The operational goal of the research, in terms of architectural work, is to point out the usefulness of studying a wide range of human behavior in everyday life, from what is known as performing the everyday, through rituals and routines, to forms of performance. The assumption is that architecture is considered a structural system of symbols in complex communicational and phenomenological constructs, as well as a multimedia textual phenomenon, which takes place in time intervals of constituting the individual and collective everyday. The general methodological theoretical position of this research is predominantly phenomenological (hermeneutic and interpretative), through which the research is conducted as qualitative interpretative research. Qualitative research assumes the involvement of researchers in the problem and the subject of research. In this case, the relevant position is the dual role of the author: as chronicler and actors of the event, which is a specific role and thus appropriate for qualitative research. The first part of the study sets a theoretical framework, followed by an analysis of the relevant concepts of architectural theory, the theory of environmental art and theory of everyday life. The final chapter of the theoretical study outlines the implications for the possibility of analyzing the ambiance of the quotidian. The second part of the thesis is conceived as qualitative research, consisting of four case studies in which previously proposed hypotheses are tested in real conditions. The concluding paragraphs are an overview of the research results obtained...Докторска теза теоријски се позиционира у пољу трансдициплинарног истраживања савремених пројектантских пракси и њихових феноменолошких интерпретација кроз појам амбијента. Тема дисертације је одређење појма архитектонског амбијента, његова теоријска поставка кроз истраживање пракси амбијенталних уметности и његова анализа кроз студију простора свакодневице у пројектима архитекте Василија Милуновића. Основни циљ истраживања је успостављање практичних релација између пракси амбијенталних уметности и архитектонског пројектовања, како би се архитектонске пројектантске праксе отвориле према критичко–истраживачким потенцијалима интердисциплинарних и хибридних пракси у уметничком стваралаштву. Корисност успостављене релације сагледава се кроз могућност интердисциплинарне размене истраживачких тактика и техника, као и кроз могућност унапређења пројектантске праксе кроз проширивање подручја и медија истраживања и деловања. Оперативни циљ дисертације је да се у оквирима архитектонског деловања укаже на корисност проучавања широког спектра људског понашања у свакодневном животу, од онога што називамо извођењем свакодневице, преко ритуала и рутина, до облика културалног извођења и перформанса. Претпоставка је да се архитектура сагледава као структура система знакова у сложеним комуникацијским и феноменолошким конструктима, као вишемедијска текстуална појавност која се одиграва у временским интервалима конституисања индивидуалне и колективне свакодневице. Општа методолошка теоријска позиција овог истраживања је доминантно феноменолошка (херменеутичка и интерпретативна), у оквиру које се истраживање спроводи као квалитативно интерпретативно истраживање. Квалитативна истраживања претпостављају укљученост истраживача у проблем и предмет истраживања, у овом случају релевантна је позиција двоструке улоге аутора као хроничара и актера догађаја, што је чини специфичном и на тај начин примереном квалитативним истраживањима. У првом делу истраживања постављен је теоријски оквир рада, анализирани су референтни концепти архитектонске теорије, теорије амбијенталних уметности и теорије свакодневице, у закључној глави теоријског истраживања износе се импликације за могућност анализе амбијената свакодневице. Други део дисертације је конципиран као квалитативно истраживање које се састоји из четири студије случаја у којима се претходно постављене тезе испитују у конкретним условима. На основу резултата истраживања, формирају се закључни ставови..

Toxicological analysis of the risk of lead exposure in metal processing

Purpose: To evaluate toxicological risks for workers who are exposed to lead in their work environment. Methods: Since it is an important indicator of toxicological risk, a statistical analysis of lead concentration and biological lead toxicity markers in blood and urine were performed for both exposed and control groups. Both experimental groups consisted of employees from "NISSAL" JSC factory. Analytical epidemiological method in the form of a retrospective cohort study was applied, and covered the period from 2001 to 2010. The concentration of lead, δ-aminolevulinic acid, and coproporphyrin in biological samples were determined by spectrophotometric methods. Results: The results showed a high positive correlation between lead concentrations in blood and urine and the length of exposure of the participants (p < 0.01). Also, increase of lead concentration in the biological material significantly increased δ-aminolevulinic acid (p < 0.01) and slightly increased the concentration of coproporphyrin, both of which are important indicators of toxicological risk. Conclusion: The control group of employees belonged to the normal risk category, while the exposed group belonged to a moderate risk category. Correlation between the monitored parameters is statistically significant at p < 0.01 and p < 0.05. Keywords: Lead, δ-Aminolevulinic acid, Coproporphyrin, Chronic exposure, Toxicity, Correlatio

Application of environmental art practices in architectural design of everyday spaces

Докторска теза теоријски се позиционира у пољу трансдициплинарног истраживања савремених пројектантских пракси и њихових феноменолошких интерпретација кроз појам амбијента. Тема дисертације је одређење појма архитектонског амбијента, његова теоријска поставка кроз истраживање пракси амбијенталних уметности и његова анализа кроз студију простора свакодневице у пројектима архитекте Василија Милуновића. Основни циљ истраживања је успостављање практичних релација између пракси амбијенталних уметности и архитектонског пројектовања, како би се архитектонске пројектантске праксе отвориле према критичко–истраживачким потенцијалима интердисциплинарних и хибридних пракси у уметничком стваралаштву. Корисност успостављене релације сагледава се кроз могућност интердисциплинарне размене истраживачких тактика и техника, као и кроз могућност унапређења пројектантске праксе кроз проширивање подручја и медија истраживања и деловања. Оперативни циљ дисертације је да се у оквирима архитектонског деловања укаже на корисност проучавања широког спектра људског понашања у свакодневном животу, од онога што називамо извођењем свакодневице, преко ритуала и рутина, до облика културалног извођења и перформанса. Претпоставка је да се архитектура сагледава као структура система знакова у сложеним комуникацијским и феноменолошким конструктима, као вишемедијска текстуална појавност која се одиграва у временским интервалима конституисања индивидуалне и колективне свакодневице. Општа методолошка теоријска позиција овог истраживања је доминантно феноменолошка (херменеутичка и интерпретативна), у оквиру које се истраживање спроводи као квалитативно интерпретативно истраживање. Квалитативна истраживања претпостављају укљученост истраживача у проблем и предмет истраживања, у овом случају релевантна је позиција двоструке улоге аутора као хроничара и актера догађаја, што је чини специфичном и на тај начин примереном квалитативним истраживањима. У првом делу истраживања постављен је теоријски оквир рада, анализирани су референтни концепти архитектонске теорије, теорије амбијенталних уметности и теорије свакодневице, у закључној глави теоријског истраживања износе се импликације за могућност анализе амбијената свакодневице. Други део дисертације је конципиран као квалитативно истраживање које се састоји из четири студије случаја у којима се претходно постављене тезе испитују у конкретним условима. На основу резултата истраживања, формирају се закључни ставови...From a theoretical standpoint, this is an interdisciplinary doctoral thesis, combining contemporary design practices with their phenomenological interpretation using the notion of atmosphere of a space. The theme of the dissertation is a definition of the concept of architectural atmosphere, its theoretical interpretation through research of environmental art practice and its analysis through the study of everyday spaces in the designs of architect Vasilije Milunović. The main research objective is to establish a practical relationship between the practices of environmental art and architectural design, in order to open architectural design practice towards critical and research potentials of interdisciplinary and hybrid practices in artistic creation. The usefulness of the established relations is reflected in the possibility of interdisciplinary exchange of research tactics and techniques, as well as through the possibility of improvement of design practice through broadening of the area and of the media of research and work. The operational goal of the research, in terms of architectural work, is to point out the usefulness of studying a wide range of human behavior in everyday life, from what is known as performing the everyday, through rituals and routines, to forms of performance. The assumption is that architecture is considered a structural system of symbols in complex communicational and phenomenological constructs, as well as a multimedia textual phenomenon, which takes place in time intervals of constituting the individual and collective everyday. The general methodological theoretical position of this research is predominantly phenomenological (hermeneutic and interpretative), through which the research is conducted as qualitative interpretative research. Qualitative research assumes the involvement of researchers in the problem and the subject of research. In this case, the relevant position is the dual role of the author: as chronicler and actors of the event, which is a specific role and thus appropriate for qualitative research. The first part of the study sets a theoretical framework, followed by an analysis of the relevant concepts of architectural theory, the theory of environmental art and theory of everyday life. The final chapter of the theoretical study outlines the implications for the possibility of analyzing the ambiance of the quotidian. The second part of the thesis is conceived as qualitative research, consisting of four case studies in which previously proposed hypotheses are tested in real conditions. The concluding paragraphs are an overview of the research results obtained..

DESIGN OF FIRST ORDER DIFFERENTIATOR WITH PARALLEL ALL-PASS STRUCTURE

In this paper a new method for design of the first order differentiator is presented. The proposed differentiator consists of two parallel branches, i.e. direct path and IIR all-pass filter. The described design method allows one to obtain solution with minimum mean relative error at the desired region by controlling the ratio of phase response extremes. A small relative magnitude error, as well as a low phase error, at low frequencies is condition for good time domain behaviour. The obtained differentiator can be realized by means of only two multipliers, hence being a good choice for real time applications. The proposed solution provides a lower magnitude error than several known differentiators with similar phase error

Ocena opravdanosti investicija u reciklažu otpada - izazovi za upravljanje održivim razvojem u Republici Srbiji

Besides the all positive effects on economic growth the modern business environment also has some disadvantages, reflected in generating different kinds of waste in large quanti ties The issue of waste has become more apparent i n the period of transition from the traditional economic into the so called consumer society It is characterized by the c onsumption of a plethora of di fferent products , which, in combination with the decreasing lifespan of products, results in an enormous pressure and negative impact on the environment , bringing sustainabl e development into jeopardy . In th ese circumstances , the development of the recycli ng industry presents itself as a possible solution to the problem of waste generation and management, while creating the basic preconditions for sustainable development. The empirical research is based on the case study of the companies that are dealing wi th recycling of cars and electrical and electronic equipment, and the cost benefit analysis is used for the evaluation of both the financial and non financial, i.e. socioeconomic benefits. The f inancial anal ysis has confirmed the justification of investmen t s in both projects, not only car recycling but also the electronic and electrical equipment recycling. From the investors’ perspective , car recycling is a more acceptable project , since it generates more profit, and it is less depend e nt on government ince ntives. The i ncentive oriented policy in the area of e waste recycling is requiredrequired since thesince the research has shown that theresearch has shown that the continuity of continuity of this process isthis process is directlydirectly dependdependentent onon governmentgovernment support. support. The The significant amount of socioeconomic benefitssignificant amount of socioeconomic benefits,, created by created by ththee electronic electronic and electrical equipment recycling projectand electrical equipment recycling project, makes it acceptable, makes it acceptable from the from the social perspective, social perspective, creating a longcreating a long--term contribution term contribution to sustainable to sustainable developmentdevelopment. The sensitivity analysis results indicate the variation in stock prices of secondary rawprices of secondary raw materials, which are the product of the materials, which are the product of the recycling process, and disparity of incentive policies towards this recycling process, and disparity of incentive policies towards this process in the Republic of Serbia represent factors which are the most process in the Republic of Serbia represent factors which are the most influential on the investment analysis criteria of recycling projects. This influential on the investment analysis criteria of recycling projects. This dissertation contributes to a better understanding of the current dissertation contributes to a better understanding of the current conditions in the recycling industry in the Republic of Serbia, and conditions in the recycling industry in the Republic of Serbia, and demonstrates the direction of future development as well as the most demonstrates the direction of future development as well as the most important challenges which stand in the way of reachiimportant challenges which stand in the way of reaching the goal of ng the goal of sustainable development.sustainable development

Discrimination of mineral waters using near-infrared spectroscopy and aquaphotomics

Voda je jedan od najčešće proučavanih materijala danas, ali uprkos tome mnoga njena svojstva i dalje ostaju nerazjašnjena i neiskorišćena. Voda je neophodna za normalno funkcionisanje ljudskog organizma, između ostalog zbog toga, poremećaji homeostaze vode u ljudskom telu leže u osnovi mnogih bolesti. Analiza vode i njene ispravnosti za upotrebu u ljudskoj ishrani uglavnom se bavi onim što je prisutno u vodi - koncentracijama prisutnih anjona i katjona, prisustvu mikroorganizama i tome slično. Različite vrste voda se uglavnom i klasifikuju upravo prema vrsti elemenata koje sadrže, koncentraciji prisutnih elemenata, ili pak odnosu između koncentracije pojedinih jona i njihov efekat na ljudski organizam razmatra se isključivo sa stanovišta elemenata koji su prisutni u njoj. Međutim, iako je poznato da voda formira različite tipove klastera i može da se organizuje oko prisutnih elemenata na različite načine, klasifikacija voda na osnovu organizacije vodenih molekula, kao i efekti različito klasterizovanih voda na ljudski organizam, za sada ne postoje u literaturi. Predmet ovog rada je diskriminacija različitih tipova voda na osnovu njihovog spektra u bliskoj infracrvenoj oblasti, primenom multivarijacione analize i novog pristupa za tumačenje spektara vode u ovoj oblasti, poznatog pod nazivom Akvafotomika. Akvafotomika interpretira spektar vode u bliskoj infracrvenoj oblasti preko posebno definisanih koordinata vodene mreže (water matrix coordinates - WAMACS) kojima su pripisani tačno određeni vibracioni modovi molekula vode preko kojih se može zaključiti kako se molekuli vode organizuju. Na taj način, primenom saznanja akvafotomike, voda se može opisati i sa aspekta njene organizacije u klastere, i time se omogućiti i diskriminacija voda na osnovu prisutnih tipova klastera što je prikazano u ovom radu.Despite that water is one of the most studied materials today its dynamic properties are still not well understood. Water state in human organism is of high importance for normal healthy functioning of human body. Different kinds of water are usually classified according to their present solutes and concentrations of these solutes, but though it is known that water molecules can form clusters around present solutes, the classification of waters based on types of water molecular organization and present clusters is not present in current literature. In this study the multivariate analysis is used for classification of commercial mineral waters based on their near-infrared spectra (NIR). Further, the aquaphotomics has been applied, a new approach for interpretation of near-infrared spectra of water, that gives insight into organization of water molecules in each of these waters

Electrical characteristics of female and male human skin

Bioimpedance spectroscopy (BIS) is a popular method for characterizing the electrical properties of biological tissues. In this study, BIS measurement data of female and male human skin were analyzed and compared. The electrical characteristics of tissue were followed according to four-parameters of the Cole-Cole model: low frequency resistance R0; high frequency resistance R∞; relaxation time t and parameter a. Individual electrical characteristics of human skin were determined for 30 women and 30 men. The distribution and one-way analysis of variance (one-way ANOVA) of the Cole-Cole parameters R0, R∞, t, a within the human population indicated their different dependence on gender. Parameter a, which is higher in the female subjects (a =0.83±0.03) than in the male subjects (a=0.7±0.05), is strongly dependent on gender (p=0). Parameter R∞ also significantly depends on gender (p=0.002), while t and R0 seem to be slightly related to gender (p>0.05). [Acknowledgments - This work was supported by the Ministry of Education and Science of the Republic of Serbia (41006).