3′-Hydroxymethyl 2′-deoxynucleoside 5′-triphosphates are inhibitors highly specific for reverse transcriptase

AbstractdNTP(3′-OCH3), a 3′-O-methyl derivative of dNTP, is a chain terminator substrate for DNA synthesis catalyzed by AMV reverse transriptase. The enzyme seems to be the only DNA polymerase susceptible to the inhibitor while all the other DNA polymerases tested are fully resistant to the nucleotide analog. The resistant polymerases are: E. coli DNA polymerase I, Klenow's fragment of DNA polymerase I, phage T4 DNA polymerase, calf thymus DNA polymerase α, rat liver DNA polymerase β and calf thymus terminal deoxyribonucleotidyl transferase

A novel and efficient method to prepare 2-aryltetrahydrofuran-2-ylphosphonic acids

A novel one-pot method was developed for the synthesis of the title compounds starting from 4-chloro-1-aryl-1-butanones 1, phosphorus trichloride and acetic acid. The end products 2 were obtained in 20–94% yield. The cyclization step under acidic conditions probably occurs as a result of anchimeric assistance of the phosphonic acid group