Environmental and Hunter-Gatherer Responses to the White River Ash East Volcanic Eruption in the Late Holocene Canadian Subarctic

The eastern lobe of the Alaskan White River Ash volcanic event of AD 846 – 848 blanketed portions of Yukon and Northwest Territories, Canada, in 5 to 50 cm of tephra. The eruption has been linked to concurrent changes among hunter- gatherers, including the spread of new technologies and the continent-wide migration of a group of Dene ancestors from Subarctic Canada to the United States. We use published palaeoenvironmental data (primarily pollen and charcoal profiles) as well as studies of modern ash fall ecology and human health hazards associated with eruptions to reconstruct effects of the White River Ash east event on northern hunter-gatherer subsistence. While many components of local ecosystems appear to have rebounded quickly from ash deposition, we deduce a more pronounced impact on the important game species of caribou and salmon, the seasonal migration paths of which were intersected by thick deposits of ash. A trophic model informed by palaeoenvironmental data and ethnohistoric records suggests that negative biological effects of the ash temporarily pushed hunter-gatherer populations to neighbouring and less affected kin groups for up to 100 years. This synthesis contextualises archaeological theories of human responses to ecological disturbance events in circumpolar landscapes.Le lobe occidental du dépôt de cendres volcaniques White River Ash, en Alaska, remontant aux années 846 – 848 A.D., a recouvert certaines parties du Yukon et des Territoires du Nord-Ouest, au Canada, d’une couche de téphra de 5 à 50 cm. Cette éruption a été liée à des changements simultanés chez les chasseurs-cueilleurs, dont l’adoption de nouvelles technologies et la migration à l’échelle du continent d’un groupe d’ancêtres dénés, de la zone subarctique canadienne jusqu’aux États-Unis. Nous avons publié des données paléoenvironnementales (principalement des profils de pollen et de charbon de bois) ainsi que des études d’écologie moderne de chutes de cendres et de dangers pour la santé des êtres humains découlant d’éruptions afin de reconstituer les effets du dépôt occidental White River Ash sur la subsistance des chasseurs-cueilleurs nordiques. Même si de nombreuses composantes des écosystèmes locaux semblent s’être remises rapidement des dépôts de cendres, nous déduisons que les répercussions ont été plus prononcées sur les espèces de gibier de caribou et sur le saumon, dont les voies de migration saisonnière croisaient les épais dépôts de cendres. Un modèle trophique établi à l’aide de données paléoenvironnementales et d’enregistrements ethnohistoriques suggère que les effets biologiques négatifs des cendres ont poussé temporairement les populations de chasseurs-cueilleurs chez des groupes apparentés avoisinants et moins touchés pendant une centaine d’années. Cette synthèse met en contexte les théories archéologiques se rapportant aux réactions humaines vis-à-vis d’événements de perturbation écologique dans les paysages circumpolaires

GRAND CHALLENGE No. 5: COMMUNICATING ARCHAEOLOGY Outreach and Narratives in Professional Practice

Communicating archaeology to non-expert audiences can convey the role and value of the discipline, implant respect for heritage, and connect descendant communities to their past. A challenge facing archaeology communicators is to translate complex ideas while retaining their richness and maximizing audience engagement. This article discusses how archaeologists can effectively communicate with non-experts using narrative and visual tools. We provide a communication strategy and three case studies from North America. The examples include the packaging of archaeological theory in the shape of mystery novels for student consumption; the use of artwork to anchor archaeological narratives in public outreach; and, the use of historical fiction to reformat archaeological content for Indigenous communities. We conclude with a discussion of outreach capacities and some of the risks and rewards of professional interactions with non-archaeologists

Effects of Ambulant Myofeedback Training and Ergonomic Counselling in Female Computer Workers with Work-Related Neck-Shoulder Complaints: A Randomized Controlled Trial

Objective: To investigate the effects of ambulant myofeedback training including ergonomic counselling (Mfb) and ergonomic counselling alone (EC), on work-related neck-shoulder pain and disability. Methods: Seventy-nine female computer workers reporting neck-shoulder complaints were randomly assigned to Mfb or EC and received four weeks of intervention. Pain intensity in neck, shoulders, and upper back, and pain disability, were measured at baseline, immediately after intervention, and at three and six months follow-up. Results: Pain intensity and disability had significantly decreased immediately after four weeks Mfb or EC, and the effects remained at follow up. No differences were observed between the Mfb and EC group for outcome and subjects in both intervention groups showed comparable chances for improvement in pain intensity and disability. Conclusions: Pain intensity and disability significantly reduced after both interventions and this effect remained at follow-up. No differences were observed between the two intervention groups

A Measure of the Promiscuity of Proteins and Characteristics of Residues in the Vicinity of the Catalytic Site That Regulate Promiscuity

Promiscuity, the basis for the evolution of new functions through ‘tinkering’ of residues in the vicinity of the catalytic site, is yet to be quantitatively defined. We present a computational method Promiscuity Indices Estimator (PROMISE) - based on signatures derived from the spatial and electrostatic properties of the catalytic residues, to estimate the promiscuity (PromIndex) of proteins with known active site residues and 3D structure. PromIndex reflects the number of different active site signatures that have congruent matches in close proximity of its native catalytic site, the quality of the matches and difference in the enzymatic activity. Promiscuity in proteins is observed to follow a lognormal distribution (μ = 0.28, σ = 1.1 reduced chi-square = 3.0E-5). The PROMISE predicted promiscuous functions in any protein can serve as the starting point for directed evolution experiments. PROMISE ranks carboxypeptidase A and ribonuclease A amongst the more promiscuous proteins. We have also investigated the properties of the residues in the vicinity of the catalytic site that regulates its promiscuity. Linear regression establishes a weak correlation (R2∼0.1) between certain properties of the residues (charge, polar, etc) in the neighborhood of the catalytic residues and PromIndex. A stronger relationship states that most proteins with high promiscuity have high percentages of charged and polar residues within a radius of 3 Å of the catalytic site, which is validated using one-tailed hypothesis tests (P-values∼0.05). Since it is known that these characteristics are key factors in catalysis, their relationship with the promiscuity index cross validates the methodology of PROMISE

Elevated basal serum tryptase identifies a multisystem disorder associated with increased TPSAB1 copy number

Elevated basal serum tryptase levels are present in 4-6% of the general population, but the cause and relevance of such increases are unknown. Previously, we described subjects with dominantly inherited elevated basal serum tryptase levels associated with multisystem complaints including cutaneous flushing and pruritus, dysautonomia, functional gastrointestinal symptoms, chronic pain, and connective tissue abnormalities, including joint hypermobility. Here we report the identification of germline duplications and triplications in the TPSAB1 gene encoding α-tryptase that segregate with inherited increases in basal serum tryptase levels in 35 families presenting with associated multisystem complaints. Individuals harboring alleles encoding three copies of α-tryptase had higher basal serum levels of tryptase and were more symptomatic than those with alleles encoding two copies, suggesting a gene-dose effect. Further, we found in two additional cohorts (172 individuals) that elevated basal serum tryptase levels were exclusively associated with duplication of α-tryptase-encoding sequence in TPSAB1, and affected individuals reported symptom complexes seen in our initial familial cohort. Thus, our findings link duplications in TPSAB1 with irritable bowel syndrome, cutaneous complaints, connective tissue abnormalities, and dysautonomia

Genome-Wide Meta-Analyses of Breast, Ovarian, and Prostate Cancer Association Studies Identify Multiple New Susceptibility Loci Shared by at Least Two Cancer Types.

UNLABELLED: Breast, ovarian, and prostate cancers are hormone-related and may have a shared genetic basis, but this has not been investigated systematically by genome-wide association (GWA) studies. Meta-analyses combining the largest GWA meta-analysis data sets for these cancers totaling 112,349 cases and 116,421 controls of European ancestry, all together and in pairs, identified at P < 10(-8) seven new cross-cancer loci: three associated with susceptibility to all three cancers (rs17041869/2q13/BCL2L11; rs7937840/11q12/INCENP; rs1469713/19p13/GATAD2A), two breast and ovarian cancer risk loci (rs200182588/9q31/SMC2; rs8037137/15q26/RCCD1), and two breast and prostate cancer risk loci (rs5013329/1p34/NSUN4; rs9375701/6q23/L3MBTL3). Index variants in five additional regions previously associated with only one cancer also showed clear association with a second cancer type. Cell-type-specific expression quantitative trait locus and enhancer-gene interaction annotations suggested target genes with potential cross-cancer roles at the new loci. Pathway analysis revealed significant enrichment of death receptor signaling genes near loci with P < 10(-5) in the three-cancer meta-analysis. SIGNIFICANCE: We demonstrate that combining large-scale GWA meta-analysis findings across cancer types can identify completely new risk loci common to breast, ovarian, and prostate cancers. We show that the identification of such cross-cancer risk loci has the potential to shed new light on the shared biology underlying these hormone-related cancers. Cancer Discov; 6(9); 1052-67. ©2016 AACR.This article is highlighted in the In This Issue feature, p. 932.The Breast Cancer Association Consortium (BCAC), the Prostate Cancer Association Group to Investigate Cancer Associated Alterations in the Genome (PRACTICAL), and the Ovarian Cancer Association Consortium (OCAC) that contributed breast, prostate, and ovarian cancer data analyzed in this study were in part funded by Cancer Research UK [C1287/A10118 and C1287/A12014 for BCAC; C5047/A7357, C1287/A10118, C5047/A3354, C5047/A10692, and C16913/A6135 for PRACTICAL; and C490/A6187, C490/A10119, C490/A10124, C536/A13086, and C536/A6689 for OCAC]. Funding for the Collaborative Oncological Gene-environment Study (COGS) infrastructure came from: the European Community's Seventh Framework Programme under grant agreement number 223175 (HEALTH-F2-2009-223175), Cancer Research UK (C1287/A10118, C1287/A 10710, C12292/A11174, C1281/A12014, C5047/A8384, C5047/A15007, C5047/A10692, and C8197/A16565), the US National Institutes of Health (CA128978) and the Post-Cancer GWAS Genetic Associations and Mechanisms in Oncology (GAME-ON) initiative (1U19 CA148537, 1U19 CA148065, and 1U19 CA148112), the US Department of Defence (W81XWH-10-1-0341), the Canadian Institutes of Health Research (CIHR) for the CIHR Team in Familial Risks of Breast Cancer, Komen Foundation for the Cure, the Breast Cancer Research Foundation, and the Ovarian Cancer Research Fund [with donations by the family and friends of Kathryn Sladek Smith (PPD/RPCI.07)]. Additional financial support for contributing studies is documented under Supplementary Financial Support.This is the author accepted manuscript. The final version is available from the American Association for Cancer Research via http://dx.doi.org/10.1158/2159-8290.CD-15-122

L'Anse aux Meadows (EjAv-01) : an archaeological and ethnohistorical investigation of bird use during the recent Indian period in Newfoundland and Labrador

This thesis explores Native American bird hunting and consumption in Newfoundland and Labrador from 2000 years ago to the nineteenth century. Ethnohistorical records of Beothuk and Innu bird use inform an archaeological interpretation of the Recent Indian period at L'Anse aux Meadows National Historic Site. This investigation of hunter-gatherer bird exploitation supplements research of Late Holocene mammal hunting in order to provide a broadened perspective of pre-contact ecology in the North Atlantic. -- Birds were attractive resources to many northern hunter-gatherers because of the predictability and availability of various bird species throughout the year. At the time of European contact, many Newfoundland Beothuk harvested seabirds while the Labrador Innu relied on ptarmigan and grouse. Based on ethnohistorical records, birds played prominent roles in the ecological systems of both Native peoples and an array of tools were utilized to capture and process them. -- A synopsis of the province's archaeological record reveals that birds commonly appear in faunal assemblages from Newfoundland Recent Indian and Beothuk coastal sites while hunting blinds are the most visible refuse of bird use in Labrador. Geographic and temporal trends of avian exploitation are discussed with reference to ethnographic and archaeological examples of northern hunter-gatherers. -- Archaeological research at L'Anse aux Meadows provides a case study of Recent Indian bird use in northern Newfoundland. Explanations are offered for the co-occurrence of a faunal record dominated by bird bone and a high relative frequency of large bifaces and scrapers. I argue that at L'Anse aux Meadows birds were hunted and tools were prepared for future activities in the seasonal round