Synthesis of ferrocene amidophosphine ligands from isocyanates

Community structure of patients with or without recurrent C. difficile infection. Principal coordinates analysis (PCoA) was used to plot the Yue and Clayton dissimilarity index (θ YC ). a The community structure of the microbiota in index (initial) samples from nonrecurrent, recurrent, and reinfected patients (analysis of molecular variance (AMOVA), p = 0.068). b The community structure of samples positive or negative for C. difficile as determined by cultivation (AMOVA, p = 0.001). (PDF 166 kb

Additional file 1: of Development and validation of a structured observation scale to measure responsiveness of physicians in rural Bangladesh

List and sources of all items in quantitative structured observation tool. (DOCX 112 kb

Traffic environment for children and elderly as pedestrians and cyclists

Godkänd; 2005; 20080821 (andbra

Additional file 1: of Development and validation of a structured observation scale to measure responsiveness of physicians in rural Bangladesh

List and sources of all items in quantitative structured observation tool. (DOCX 112 kb

Correlation between CD24% and cisplatin sensitivity.

<p>A) Tabulation showing that CD24+ is an indicator of poor outcome in multiple cancers. B) Tabulation showing that the positive or negative expression of CD24 is the discerning factor in isolating cancer stem cells/ tumor propagating cells in various cancers. C) Flow cytometry analysis of membrane bound CD24 and CD44 of the three laryngeal carcinoma lines show an increasing fraction of CD24% and D) Graphical representation of CD24% with R² = 0.804 showing a positive linear correlation with increasing cisplatin resistance in the three laryngeal carcinoma lines. E) Primary and secondary orospheres formed by the CD24+ (green) and CD24– (red) fractions. F) Graph of the number of orospheres formed as shown in E) with standard error of mean (t-test, n = 3). G) CD24+ residual fraction increase in proportion as it remains viable even after an IC-50 dose of cisplatin treatment. H) Graph with standard error of mean (t-test, n = 3) of the percent increase in proportion of the residual viable CD24+ fraction after an IC-50 dose of cisplatin treatment.</p

Modulation of CD24 expression directly affects the gene expression of various pro-survival genes critical in cisplatin resistance.

<p>A) and B) Down regulation of CD24 in CD24 rich UM-SCC-10B and UM-SCC-15s leads to significant down regulation of critical pro-survival genes, standard error of mean (t-test, n = 3). C) Up regulation of CD24 in CD24 deficient UM-SCC-74B leads to significant up regulation of critical pro-survival genes, standard error of mean (t-test, n = 3). D) Graph of the number of orospheres formed by the CD24 knockdown variants of UM-SCC-10B and UM-SCC-15s before and after an IC-50 dose of cisplatin treatment, standard error of mean (t-test, n = 3). E) Graph of the number of orospheres formed by the CD24 overexpressing variants of UM-SCC-74B before and after an IC-50 dose of cisplatin treatment, standard error of mean (t-test, n = 3).</p

No Correlation between CD44% and cisplatin sensitivity.

<p>A) Hematoxylin and Eosin staining of three laryngeal carcinoma cell lines. B) Cisplatin IC-50 calculated for the three laryngeal carcinoma lines. C) Flow cytometry analysis of membrane bound CD44 on the three laryngeal carcinoma lines and D) graphical representation of CD44% with standard error of mean (t-test, n = 3). E) Graph of the number of orospheres formed with standard error of mean (t-test, n = 3).</p

CD24 plays a functional role in determining cisplatin sensitivity of UM-SCC-74B.

<p>A) CD24 expression Lentivector map purchased from GeneCopoeia (US). B) RNA level confirmation of CD24 up-regulation in UM-SCC-74B, standard error of mean (t-test, n = 3). C) Protein level confirmation of CD24 up-regulation in UM-SCC-74B. D) Cisplatin IC-50 value CD24 overexpressing UM-SCC-74B rises significantly as compared to the parental UM-SCC-74B. E) Graph showing cisplatin IC-50 value of CD24 overexpressing UM-SCC-74B rises significantly as compared to the parental UM-SCC-74B, standard error of mean (t-test, n = 3).</p

CD24 expression and its correlation with cisplatin treatment outcome.

<p>A) Tumor volumes of UM-SCC-10B vector control and its CD24 knockdown clone UM-SCC-10B CD24KD treated with Saline (Control) and Cisplatin (Test), standard error of mean (t-test, n = 8). B) Tumor volumes of UM-SCC-15s vector control and its CD24 knockdown clone UM-SCC-15s CD24KD treated with Saline (Control) and Cisplatin (Test), standard error of mean (t-test, n = 8). C) Tabulation tumor volumes measured for UM-SCC-10B and UM-SCC-10BCD 24KD; graph of the final volume of the tumors and their statistical measure (standard error of mean). D) C) Tabulation tumor volumes measured for UM-SCC-15s and UM-SCC-15s CD24KD; graph of the final volume of the tumors and their statistical measure (standard error of mean).</p

Real time PCR primers to probe gene expression in lines with modified CD24 expression levels.

<p>Real time PCR primers to probe gene expression in lines with modified CD24 expression levels.</p