Contribution of Human Papillomavirus E6 PDZ-Binding Activity to Virus-Induced Pathogenesis

It is clear that the targeting of PDZ-containing substrates by E6 is important for the normal viral life cycle and for the progression to malignancy. However, which of these PDZ domain-containing proteins is relevant for HPV pathology is still elusive. In this study, we provide the evidence that different PDZ domain-containing proteins are differentially targeted by E6. With these experiments, we identified MAGI-1 as a sensitive proteolytic substrate for both the HPV-16 and HPV-18 E6 oncoproteins. We show that E6 promotes the degradation of membrane-bound and nuclear pools of MAGI-1, and the silencing of E6 expression resulted in the MAGI-1-mediated junctional recruitment of ZO-1. Using a mutant MAGI-1, resistant to E6-mediated degradation, we also show that its expression in HeLa cells also promotes membrane recruitment of the tight junction-associated proteins ZO-1 and PAR3, represses cell proliferation and promotes apoptosis. These findings suggest that E6-mediated inhibition of MAGI-1 function perturbs tight junction assembly, with concomitant stimulation of proliferation and inhibition of apoptosis. We also found that the cell polarity regulator hScrib is differentially targeted by HPV-16 and HPV-18 Ed-mediated degradation. Surprisingly, we found that residual levels of hScrib expression are required for the maintenance of high levels of HPV-18 E6 expression in HeLa cells. This is not due to an effect on E6 stability or transcription, but rather is due to an effect upon E6 translation. We provide evidence that hScrib and E6 both regulate the PI3K/mTORC1 pathway, and that hScrib might regulate cap-dependent translation through the modulation of the mTORCl effector S6 kinase. This provides an unexpected role for hScrib in the regulation of tissue homeostasis, and provides further evidence that E6, by fine-tuning the levels of expression of its different cellular substrates, can impact upon a wide range of biological processes implicated in the pathogenesis of cervical cancer

Demonstration experiments in physics education: Energy change

U okviru rada diskutiraju se metode izvedbe nastave fizike koje bi trebale rezultirati boljim razumijevanjem nastavnih sadržaja i stjecanjem trajnih znanja. Pri tome je ključan fizikalni pokus kao središnji element nastavnog sata fizike. Na primjeru teme o pretvorbi energije temeljene na zakonu očuvanja energije, daje se pregled odabranih demonstracijskih pokusa kojima bi se mogli postići navedeni ciljevi.In this work, methods of performing physical teaching are discussed, which should result in a better understanding of teaching content and the acquisition of persistent knowledge. The physical experiment has a key role as the central element of the physics teaching. In an example of energy-based transformation topic, an overview of selected demonstration experiments is provided to achieve these goals

Sustainability in fasion as inspiration for textile design

Istraživanjem problema održivosti mode, jasno je da su nam potrebna riješenja kako bi smanjili štetu koju modna industrija prouzrokuje svojom proizvodnjom i stvaranjem otpada. Kroz povratak u prošlost i ponovno razmatranje starih tehnika bojadisanja te korištenje biljaka koje su najbolji i najraznolikiji izvor bojila, istražujem nove tehnike i mogučnosti printanja tekstila bez kemikalija te težim recikliranju 100% upotrebljene sirovine što rezultira stvaranjem kolekcije tekstila zero-waste (nula otpada) kvalitete.By researching the problem of sustainability of fashion, it is clear that we need solutions to reduce the damage caused by the fashion industry through its production and waste generation. Through returning to the past and reconsidering the old dyeing techniques and the use of the best and most colorful plants, I research the new techniques and the possibilities of printing textiles without chemicals and trying to recyclle 100% of the raw material used, which results with the creation of a zero-waste textile collection

Differences in self-regulated learning between gifted students, students with special needs and other students in Slovenian schools

Self-regulated learning strategies play a crucial role in learning progress and academic achievement of different groups of students. The purpose of the present study is to investigate differences in the use of self-regulatory strategies among a sample of 1,495 students, aged 12 to 15 years, representing three groups: gifted students, students with special needs, and other students. The theoretical framework for the study is Pintrich\u27s (1991) model of self-regulated learning. Data were collected using the Motivated Strategies for Learning Questionnaire (MSLQ). Results indicated that gifted students scored significantly higher on the MSLQ subscales of motivation and learning strategies than students with special needs and other peers. Special needs students reported lower intrinsic and extrinsic goal orientation and weaker self-efficacy in learning and achievement than other students. There were no significant differences between these two groups on the MSLQ learning strategies subscales. Positive and statistically significant associations between the MSLQ subscales and final grades in three school subjects (Slovenian, mathematics, and foreign language) were also confirmed. We discuss the implications of our findings for future research and the educational context that contributes most to the development of self-regulated learning in all groups of students

Late Thrombosis of Sirolimus-Eluting Stent: A Multifactorial Problem

We report a case of a young patient in whom a sirolimus-eluting stent was implanted on the culprit left anterior descending coronary artery at primary percutaneous coronary intervention (PCI) for acute myocardial infarction. Nine months later she suffered from a reinfarction due to the late stent thrombosis despite a continuous antiplatelet therapy with aspirin and clopidogrel. A cluster of factors that might have contributed to the development of the stent thrombosis were identified: suboptimal PCI technique, complete stent fracture, and clopidogrel resistance. The obstructed stent was successfully reopened by repeat PCI, while the clopidogrel maintenance dosage was doubled to 150 mg daily for the following year. The further long-term clinical course was uneventful

Are Temporal Concepts Embodied? A Challenge for Cognitive Neuroscience

Is time an embodied concept? People often talk and think about temporal concepts in terms of space. This observation, along with linguistic and experimental behavioral data documenting a close conceptual relation between space and time, is often interpreted as evidence that temporal concepts are embodied. However, there is little neural data supporting the idea that our temporal concepts are grounded in sensorimotor representations. This lack of evidence may be because it is still unclear how an embodied concept of time should be expressed in the brain. The present paper sets out to characterize the kinds of evidence that would support or challenge embodied accounts of time. Of main interest are theoretical issues concerning (1) whether space, as a mediating concept for time, is itself best understood as embodied and (2) whether embodied theories should attempt to bypass space by investigating temporal conceptual grounding in neural systems that instantiate time perception

Revealing Levinas: Transcending moral and aesthetic distinctions between form and content through poetic gamespersonship

This poem was inspired by the confluence of two communications: An email regarding proposed changes to university workload policies, and a call for papers for a journal’s special issue on the work of Emmanuel Levinas. We used poetic form to reflect figuratively on academic integrity and what counts literally as good Levinas scholarship

Journal Data Policies: Are Croatian Journals Following Trends?

The aim of this work is to give an overview of recent developments of journal data sharing policies, with summary and examples of standardised guidelines for journal publishers. In addition, it examines the prevalence of journal data policies in Croatian journals and explores the content of these policies. To give an overview of the current state of data sharing policies, published articles that review existing journal data policies and develop model data policies or guidelines for journals were identified and examined. For the analysis of Croatian journals, data was collected from the Hrčak portal, using a software script for harvesting journal metadata and attached files from the portal. Searching for content related to data archiving through downloaded files was done using the following keywords: 'data', 'deposit', 'archiving', 'supplement', including Croatian variants and different grammar forms. The search process was facilitated by using software tools that extracted lines of text from source documents containing defined keywords, together with two lines of text above and below the position of keywords in the text as context. The script parses through documents and creates one file containing file name of the identified document and snippets of extracted text from that document. Created file is than manually examined to identify journals that have any content related to research data and eliminate content that is related to data in another context. A dataset is created which contains journal metadata and coded information about the content of the policy. Coding framework for the analysis of content related to research data in journal editorial documents was developed based on previous research (1, 2) and adapted for this analysis. Results and Discussion Recent studies (1, 2, 3) show lack of clear data sharing and transparency policies in the majority of journals. Where the policies were present, wide variety in quality of existing policies was found. This is an obstacle in the practice of data sharing, especially for the authors who need clear guidelines on how to deposit and make their data transparent and available for others to re-use. Standardisation of data policies could help journal editors and research funders to formulate clear mandates and recommendations that can influence the development of research transparency culture. Several attempts to develop a model data policies are identified in recent years, and the most prominent existing implementation guidelines for journals, publishers and funders are: Research data for journal editors by the Australian National Data Service(4), Transparency and Openness Promotion (TOP) Guidelines by the Center for Open Science(5), Research Data Policy Framework for all journals and publishers by Data policy standardisation and implementation Interest Group (IG) of the Research Data Alliance (RDA)(6), Journal Research Data Policy Model Framework by The Journal Research Data (JoRD) Project, funded by JISC (Joint Information Systems Committee)(2). These guidelines identify the key elements of a good data policy such as data citation, data repositories, data availability statements, data standards and formats, and peer review of research data. Although all of them attempt to establish standard features, they provide flexibility for adoption depending on disciplinary variation. In order to find out if Croatian journals are implementing and promoting data sharing policies, the analysis of data policies of Croatian journals was conducted. Preliminary results, based on a sample of editorial documents, show that around 15% of journals mention research data in their policy and guidance documents, whether the statement expresses only the general principle of research transparency or the statement is a requirement. Journals that explicitly mention data access and retention are usually referring to the outdated ALPSP-STM Statement on data and databases. Conclusion Data sharing is encouraged in contemporary research environment. Journals in the stage of planning to implement research data policy might find it very useful to consult existing guidelines and follow examples of good practice presented in this work. Some Croatian journals started to adopt basic data sharing policies and transparency principles, but often it is not clear how and if the existing policies are enforced

The effect of language impairment on non-symbolic exact quantity representation

Both English-speakers whose access to number language is artificially compromised by verbal interference and thePirah ̃a (an Amazonian tribe without exact number words) appear to rely on analog magnitude estimation for representing non-symbolic exact quantities greater than 3. Here, 10 participants with aphasia from stroke performed the same 5 counting tasksfrom these previous studies. Performance was poorest when targets were not visible during response (70% correct) and bestwhen targets were presented as subitizable groups of 2 and 3 (98% correct). Western Aphasia Battery-Revised subtest scoreswere reliably correlated with performance across counting tasks suggesting ways that both speech and naming may contributeto errors. Coefficients of variation for particular tasks, and significant correlations between target magnitude with both errorrate and size across tasks suggests use of analog magnitude estimation for verbally impaired participants. Diverse forms oflanguage impairment may contribute to errors on nonverbal counting tasks

Impact of the WTO’s general agreement on trade in services on public libraries

Posljednjih godina knjižničarska zajednica s velikim zanimanjem i zabrinutošću prati rad Svjetske trgovinske organizacije i pregovore o Općem sporazumu o trgovini uslugama. U radu su prikazana pitanja vezana uz taj sporazum, važna za knjižničare i o kojima trebaju u budućnosti voditi brigu – definicija, obuhvat i klasifikacija usluga. Ukazuje se i na nedovoljnu transparentnost rada Svjetske trgovinske organizacije i pregovora vezanih uz Opći sporazum o trgovini uslugama.In the last few years, the library community has been following the work of World’s Trade Organization (WTO) and its General Agreement on Trade in Services (GATS) with the great interest and concern. The paper presents questions related to GATS that are of special interest to the librarians and that they should pay good attention to in the future: definiton of services excluded from GATS, range and classification of services, and unadequate trancparancy of WTO negotiation processes concerning the GATS