Screening of Satureja subspicata Vis. honey by HPLC-DAD, GC-FID/MS and UV/VIS: prephenate derivatives as biomarkers

The samples of Satureja subspicata Vis. honey were confirmed to be unifloral by melissopalynological analysis with the characteristic pollen share from 36% to 71%. Bioprospecting of the samples was performed by HPLC-DAD, GC-FID/MS, and UV/VIS. Prephenate derivatives were shown to be dominant by the HPLC-DAD analysis, particularly phenylalanine (167.8 mg/kg) and methyl syringate (MSYR, 114.1 mg/kg), followed by tyrosine and benzoic acid. Higher amounts of MSYR (3-4 times) can be pointed out for distinguishing S. subspicata Vis. honey from other Satureja spp. honey types. GC-FID/MS analysis of ultrasonic solvent extracts of the samples revealed MSYR (46.68%, solvent pentane/Et2O 1:2 (v/v); 52.98%, solvent CH2Cl2) and minor abundance of other volatile prephenate derivatives, as well as higher aliphatic compounds characteristic of the comb environment. Two combined extracts (according to the solvents) of all samples were evaluated for their antioxidant properties by FRAP and DPPH assay; the combined extracts demonstrated higher activity (at lower concentrations) in comparison with the average honey sample. UV/VIS analysis of the samples was applied for determination of CIE Lab colour coordinates, total phenolics (425.38 mg GAE/kg), and antioxidant properties (4.26 mmol Fe2+/kg (FRAP assay) and 0.8 mmol TEAC/kg (DDPH assay)

Comprehensive Neighborhood Portraits and Child Asthma Disparities Introduction

Objectives Previous research has established links between child, family, and neighborhood disadvantages and child asthma. We add to this literature by first characterizing neighborhoods in Houston, TX by demographic, economic, and air quality characteristics to establish differences in pediatric asthma diagnoses across neighborhoods. Second, we identify the relative risk of social, economic, and environmental risk factors for child asthma diagnoses. Methods We geocoded and linked electronic pediatric medical records to neighborhood-level social and economic indicators. Using latent profile modeling techniques, we identified Advantaged, Middle-class, and Disadvantaged neighborhoods. We then used a modified version of the Blinder-Oaxaca regression decomposition method to examine differences in asthma diagnoses across children in these different neighborhoods. Results Both compositional (the characteristics of the children and the ambient air quality in the neighborhood) and associational (the relationship between child and air quality characteristics and asthma) differences within the distinctive neighborhood contexts influence asthma outcomes. For example, unequal exposure to PM2.5 and O3 among children in Disadvantaged and Middle-class neighborhoods contribute to asthma diagnosis disparities within these contexts. For children in Disadvantaged and Advantaged neighborhoods, associational differences between racial/ethnic and socioeconomic characteristics and asthma diagnoses explain a significant proportion of the gap. Conclusions for Practice Our results provide evidence that differential exposure to pollution and protective factors associated with non-Hispanic White children and children from affluent families contribute to asthma disparities between neighborhoods. Future researchers should consider social and racial inequalities as more proximate drivers, not merely as associated, with asthma disparities in children

Evaluation of best practices for landrace conservation: farmer evaluation

Traditional farming systems and conservation of local cultivars and associated indigenous knowledge are under threat and growing pressure resulting in genetic erosion of crop diversity. These systems are an essential component of sustainable crop production, household income and human nutrition for many of the poor farmers found in fragile semi-arid ecosystems of sub-Saharan Africa (SSA). With the signing of the Convention on Biological Diversity (CBD) in 1992, in situ conservation for crops and their related genetic resources has been given prominent mention in global and national policies for biodiversity conservation. In situ strategies are an important and complementary component of the overall agrobiodiversity conservation efforts that aim to conserve not only crop genetic resources but also crop evolutionary processes. However, policy support of the science and practice of in situ conservation, lag behind CBD commitment in much of SSA. The need for activities on in situ conservation of plant genetic resources is emphasized in the CBD and in Agenda 21. Article 2 of the Convention specifically includes reference to domesticated or cultivated species. This is also anticipated in Article 8, which requires signatory Nations to “preserve and maintain knowledge, innovations and practices of indigenous and local communities embodying traditional lifestyles relevant for the conservation and sustainable use of biological diversity …” Agenda 21 reflects this commitment to in situ conservation as an essential component of sustainable agriculture, and in Chapter 14, notes the need for establishing programmes and policies to strengthen in situ conservation. In its second meeting, the Conference of the Parties to the CBD (COP 2) identified implementation of Article 8 of the CBD as a high priority and reaffirmed the importance of regional and international cooperation for the implementation of this Article. It also stressed the importance of the exchange of relevant information and experience among all stakeholders on measures taken for its implementation (Decision II/7 of COP 2). Many national PGR programmes in SSA are unable to meet their obligations towards in situ (more specifically on-farm) conservation as stated in the CBD and the Global Plan of Action (GPA) because of ineffective enabling of national policy environments that do not support traditional farming systems and in situ conservation on-farm. In order to strengthen the capacity of countries to implement effective policy, Bioversity International and national programme partners developed a project on “Community-based management of plant genetic resources in arid and semi-arid areas of Africa” funded by the Global Environment Facility (GEF) through the United Nations Environment Programme UNEP). The countries involved in the project were Benin, Burkina Faso, Ghana, Kenya, Malawi, Mali, Uganda and Zimbabwe. Through case studies, the project analysed farming systems in semi-arid ecosystems in these countries, focussing on how these systems supported the conservation of landraces of local and global significance. The methodology described in the present publication was designed within this project to draw out ‘best’ practices on how landraces have been incorporated into farming systems and/or national agricultural policies and biodiversity conservation strategies. On one hand, the farmer or resource user determines what makes the practice the ‘best one’, and they base their choices on a survival strategy or utilitarian point of view. On the other hand, the scientists on the project primarily judged how effective the practices are in conserving agrobiodiversity at different levels. The project attempted to reconcile the two views in developing frameworks for the determination of ‘best practice’

Impacts of AKST on development and sustainability goals.

Assessment and analysis of AKST impacts; Agriculture productivity, production factors and consumption; impacts of akst on sustainability, through integrated technologies and the delivery of ecosystem services and public goods; Impacts of akst on livehoods, capacoty strenthening and empowerment; relationshipsbetween akst, coordination and regulatory processes among multipl3e stakeholders

UrbanFoodplus – African-German Partnership to enhance resource use efficiency in urban and peri-urban agriculture for improved food security in West African cities

Food security in West Africa not only depends on productivity increases in marginal rural areas, but also on enhanced use of intensively farmed agricultural “niche” lands such as the urban and peri-urban spaces. They are characterised by easy market access and input availability which allows self-reinforcing processes of agricultural intensification. However, too little is known about resource use efficiencies, matter flows and negative externalities in these systems. Starting from general assessments (status quo analyses), the African-German UrbanFoodPlus (UFP) network develops and tests site-specific, farmer-tailored innovations. These directly address the above mentioned knowledge gaps in the fourWest African cities of Ouagadougou (Burkina Faso), Tamale (Ghana), Bamako (Mali), and Bamenda (Cameroon). At all locations farmers attempt to cope with increasing land pressure by cultivating along electrical power lines, on public property, and on undeveloped private land

Unlocking Phacelia tanacetifolia Benth. honey characterization through melissopalynological analysis, color determination and volatiles chemical profiling

Phacelia tanacetifolia Benth. honey (14 samples) collected in Poland was characterized by melissopalynological analysis, color determination (CIE L*a*b*Cab*hab° coordinates) and volatiles (VOCs) composition. VOCs were isolated by headspace solid-phase microextraction (HS-SPME, two fibers) and ultrasound-assisted solvent extraction (USE, two solvents) and analyzed by GCâ\u80\u93MS. Principal component analysis (PCA) and hierarchical-tree clustering (HTC) were applied to show trends and form groups and to indicate the most representative unifloral samples. Six samples were pointed out with average pollen 74.9% and color parameters (L = 85.1; a* = â\u88\u92 0.8; b* = 27.9; Cab* = 27.9; hab* = 91.9) that were significantly correlated. High abundance of trans-linalool oxide (27.3â\u80\u9345.9%) that was significantly correlated with the pollen percentages, hexan-1-ol (4.4â\u80\u935.7%) and lavender lactone (0.8% - 1.5%) were characteristic for their headspace. C13-norisoprenoids, mainly (E)-/(Z)-3-oxo-retro-α-ionol (4.7â\u80\u935.4%; 6.9â\u80\u939.4%) and vomifoliol (9.0â\u80\u9313.0%) dominated in their USE extracts

Characterization of summer savory (Satureja hortensis L.) honey by physico-chemical parameters and chromatographic/spectroscopic techniques (GC-FID/MS, HPLC-DAD, UV/VIS and FTIR-ATR

Satureja hortensis L. unifloral honey was characterized by pollen analysis, electrical conductivity, pH and extensively by chromatographic and spectroscopic techniques. UV / VIS spectro-scopy measurements revealed total phenol content of 682.1 mg GAE / kg by Folin-Ciocalteu assay, antiox-idant capacity by DPPH assay of 1.7 mmol TEAC / kg and by FRAP assay of 4.3 mmol Fe2+ / kg as well as CIE L*a*b*Cab*h°ab chromaticity coordinates. GC-MS after headspace solid-phase microextraction (HS-SPME) revealed hotrienol (22.8 %) along with other linalool derivatives, benzaldehyde (6.1 %), phenylacetaldehyde (4.9 %) and few norisoprenoids (safranal (7.6 %) as the major). Ultrasonic solvent ex-traction (USE) followed by GC-MS allowed identification of methyl syringate (54.7 %) as predominant compound along with other benzene derivatives. HPLC-DAD analysis determined tyrosine (382.0 mg kg−1), phenylalanine (140.4 mg kg−1) and methyl syringate (39.32 mg kg−1). Methyl syringate and hotrienol can be considered non-specific chemical markers of S. hortensis honey. FTIR-ATR spectral characteristics of S. hortensis honey in fingerprinting region were not significantly different from other honey types, but the integrated intensity of the region was smaller than in other unifloral honeys

Quality attributes and fatty acid, volatile and sensory profiles of “Arbequina” hydrosostainable olive oil

16 páginas.- 1 figura.- 5 tablas.- 46 refencias.- This article belongs to the Special Issue Bioactives and Functional Ingredients in Foods and BeveragesThe use of deficit irrigation techniques on olive orchards is the main trend aiming to optimize water savings while improving functional and sensory characteristics of oils from trees under deficit irrigation techniques. The brand hydroSOStainable has been defined for crops produced under water restriction conditions. HydroSOStainable olive oils obtained under two new regulated deficit irrigation and one sustained deficit irrigation treatments in “Arbequina” olive trees were evaluated by analyzing quality parameters, antioxidant activity, total phenol content, fatty acid profile, volatile compounds, and sensory descriptors. Results showed that some of these irrigation strategies improved the phenol content at “moderate” stress levels, slightly enriched the fatty acid profile (~3.5% increased oleic acid and simultaneously decreased saturated fatty acids), and increased some key volatile compounds and also several key sensory attributes. Therefore, hydroSOStainable olive oil may be more attractive to consumers as it is environmentally friendly, has a higher content of several bioactive compounds, and has improved sensory characteristics as compared to control (fully irrigated) oils. © 2019 by the authors.The study has been funded (Spanish Ministry of Economy, Industry, and Competitiveness) a coordinated research project (hydroSOS mark), including the Universidad Miguel Hernández de Elche (AGL2016-75794-C4-1-R, hydroSOS foods) and the Universidad de Sevilla (AGL2016-75794-C4-4-R) (AEI/FEDER, UE).Peer reviewe