Sudden approximation applied to rotational excitation of molecules by atoms. ii- scat- tering of polar diatomics

Sudden approximation applied to computation of rotational transition probability and inelastic total cross sections for scattering of polar and nonpolar diatomic molecules by atom

Modulation of plant growth in vivo and identification of kinase substrates using an analog-sensitive variant of CYCLIN-DEPENDENT KINASE A;1

BACKGROUND: Modulation of protein activity by phosphorylation through kinases and subsequent de-phosphorylation by phosphatases is one of the most prominent cellular control mechanisms. Thus, identification of kinase substrates is pivotal for the understanding of many – if not all – molecular biological processes. Equally, the possibility to deliberately tune kinase activity is of great value to analyze the biological process controlled by a particular kinase. RESULTS: Here we have applied a chemical genetic approach and generated an analog-sensitive version of CDKA;1, the central cell-cycle regulator in Arabidopsis and homolog of the yeast Cdc2/CDC28 kinases. This variant could largely rescue a cdka;1 mutant and is biochemically active, albeit less than the wild type. Applying bulky kinase inhibitors allowed the reduction of kinase activity in an organismic context in vivo and the modulation of plant growth. To isolate CDK substrates, we have adopted a two-dimensional differential gel electrophoresis strategy, and searched for proteins that showed mobility changes in fluorescently labeled extracts from plants expressing the analog-sensitive version of CDKA;1 with and without adding a bulky ATP variant. A pilot set of five proteins involved in a range of different processes could be confirmed in independent kinase assays to be phosphorylated by CDKA;1 approving the applicability of the here-developed method to identify substrates. CONCLUSION: The here presented generation of an analog-sensitive CDKA;1 version is functional and represent a novel tool to modulate kinase activity in vivo and identify kinase substrates. Our here performed pilot screen led to the identification of CDK targets that link cell proliferation control to sugar metabolism, proline proteolysis, and glucosinolate production providing a hint how cell proliferation and growth are integrated with plant development and physiology. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-016-0900-7) contains supplementary material, which is available to authorized users

Comparison and Appraisal of Approximation Formulas for Total Elastic Molecular Scattering Cross Sections

The Massey—Mohr (MM), Schiff (S), and Landau—Lifshitz (LL) approximations for the total elastic cross section (Q) are intercompared. All can be shown to follow from the same assumption, (i.e., the classical small‐angle deflection function, thence the Jeffreys—Born phases via the semiclassical equivalence relationship), sufficing to determine the velocity dependence of Q. Thus, for V=±C(s)/rs, Q(s)=p(s)[C(s)/hv ]2/(s—1) The coefficient p(s) is the same for the S and LL approximations; the ratio pSLL(s)/pMM(s)≥1, (<1.075); it is 1.0709 and 1.0458 for s=6 and 12, respectively.A numerical calculation for a repulsive (s=12) interaction shows that the SLL formula reproduces the partial‐wave calculated Q to within ☒%. A graphical presentation suggests the generality of this result; it also indicates the source of bias in the MM approximation. For a ``realistic'' intermolecular potential, (restricting consideration to collisions in the ``thermal'' energy range), the influence of the repulsion is only to produce undulations in Q(v); the correct value of C(6) may be obtained by velocity averaging the ``apparent'' CSLL(6).Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/70541/2/JCPSA6-38-10-2507-1.pd

Single Top Quark Production and Decay at Next-to-leading Order in Hadron Collision

We present a calculation of the next-to-leading order QCD corrections, with one-scale phase space slicing method, to single top quark production and decay process $p\bar{p},pp\to t\bar{b}+X\to b\ell\nu\bar{b}+X$ at hadron colliders. Using the helicity amplitude method, the angular correlation of the final state partons and the spin correlation of the top quark are preserved. The effect of the top quark width is also examined.Comment: 47 pages, 9 figure

Single pulse and profile variability study of PSR J1022+1001

Millisecond pulsars (MSPs) are known as highly stable celestial clocks. Nevertheless, recent studies have revealed the unstable nature of their integrated pulse profiles, which may limit the achievable pulsar timing precision. In this paper, we present a case study on the pulse profile variability of PSR J1022+1001. We have detected approximately 14,000 sub-pulses (components of single pulses) in 35-hr long observations, mostly located at the trailing component of the integrated profile. Their flux densities and fractional polarisation suggest that they represent the bright end of the energy distribution in ordinary emission mode and are not giant pulses. The occurrence of sub-pulses from the leading and trailing components of the integrated profile is shown to be correlated. For sub-pulses from the latter, a preferred pulse width of approximately 0.25 ms has been found. Using simultaneous observations from the Effelsberg 100-m telescope and the Westerbork Synthesis Radio Telescope, we have found that the integrated profile varies on a timescale of a few tens of minutes. We show that improper polarisation calibration and diffractive scintillation cannot be the sole reason for the observed instability. In addition, we demonstrate that timing residuals generated from averages of the detected sub-pulses are dominated by phase jitter, and place an upper limit of ~700 ns for jitter noise based on continuous 1-min integrations.Comment: 13 pages, 20 figures, 3 tables, accepted for publication in MNRA

The optimal schedule for pulsar timing array observations

In order to maximize the sensitivity of pulsar timing arrays to a stochastic gravitational wave background, we present computational techniques to optimize observing schedules. The techniques are applicable to both single and multi-telescope experiments. The observing schedule is optimized for each telescope by adjusting the observing time allocated to each pulsar while keeping the total amount of observing time constant. The optimized schedule depends on the timing noise characteristics of each individual pulsar as well as the performance of instrumentation. Several examples are given to illustrate the effects of different types of noise. A method to select the most suitable pulsars to be included in a pulsar timing array project is also presented.Comment: 16 pages, 6 figures, accepted by MNRA

(2,2)-Formalism of General Relativity: An Exact Solution

I discuss the (2,2)-formalism of general relativity based on the (2,2)-fibration of a generic 4-dimensional spacetime of the Lorentzian signature. In this formalism general relativity is describable as a Yang-Mills gauge theory defined on the (1+1)-dimensional base manifold, whose local gauge symmetry is the group of the diffeomorphisms of the 2-dimensional fibre manifold. After presenting the Einstein's field equations in this formalism, I solve them for spherically symmetric case to obtain the Schwarzschild solution. Then I discuss possible applications of this formalism.Comment: 2 figures included, IOP style file neede