Bolha? : uma análise do setor imobiliário no Brasil

Este artigo tem como propósito investigar a possibilidade de existência de uma bolha no mercado imobiliário brasileiro. Para consecução deste objetivo foram utilizadas variadas metodologias, empregando análises tanto em nível macro quanto microeconômico. Foram apresentadas as causas que levaram o estouro da bolha especulativa nos Estados Unidos, vinculando-as com possíveis traços semelhantes existentes na política brasileira de fomento ao crédito imobiliário. Posteriormente, foi feito um estudo acerca da conjuntura econômica no país, mediante apreciação de indicadores, déficit habitacional e crédito imobiliário. De maneira geral, após a realização da pesquisa, os resultados apontam evidências que tornam possível a existência de uma bolha imobiliária no Brasil

Genomics and epidemiology of the P.1 SARS-CoV-2 lineage in Manaus, Brazil

Cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in Manaus, Brazil, resurged in late 2020 despite previously high levels of infection. Genome sequencing of viruses sampled in Manaus between November 2020 and January 2021 revealed the emergence and circulation of a novel SARS-CoV-2 variant of concern. Lineage P.1 acquired 17 mutations, including a trio in the spike protein (K417T, E484K, and N501Y) associated with increased binding to the human ACE2 (angiotensin-converting enzyme 2) receptor. Molecular clock analysis shows that P.1 emergence occurred around mid-November 2020 and was preceded by a period of faster molecular evolution. Using a two-category dynamical model that integrates genomic and mortality data, we estimate that P.1 may be 1.7- to 2.4-fold more transmissible and that previous (non-P.1) infection provides 54 to 79% of the protection against infection with P.1 that it provides against non-P.1 lineages. Enhanced global genomic surveillance of variants of concern, which may exhibit increased transmissibility and/or immune evasion, is critical to accelerate pandemic responsiveness

Bolha? : uma análise do setor imobiliário no Brasil

Este artigo tem como propósito investigar a possibilidade de existência de uma bolha no mercado imobiliário brasileiro. Para consecução deste objetivo foram utilizadas variadas metodologias, empregando análises tanto em nível macro quanto microeconômico. Foram apresentadas as causas que levaram o estouro da bolha especulativa nos Estados Unidos, vinculando-as com possíveis traços semelhantes existentes na política brasileira de fomento ao crédito imobiliário. Posteriormente, foi feito um estudo acerca da conjuntura econômica no país, mediante apreciação de indicadores, déficit habitacional e crédito imobiliário. De maneira geral, após a realização da pesquisa, os resultados apontam evidências que tornam possível a existência de uma bolha imobiliária no Brasil

Pharmacological blockade of protease-Activated Receptor 2 improves airway remodeling and lung inflammation in experimental allergic asthma

Protease-activated receptors (PARs) are metabotropic G-protein-coupled receptors that are activated via proteolytic cleavage of a specific sequence of amino acids in their N-terminal region. PAR2 has been implicated in mediating allergic airway inflammation. This study aims to study the effect of PAR2 antagonist ENMD1068in lung inflammation and airway remodeling in experimental asthma. Allergic lung inflammation was induced in sensitized BALB/c mice through intranasal instillations of ovalbumin (OVA), and mice were pretreated with ENMD1068 1 hour before each OVA challenge. Bronchoalveolar lavage fluid (BALF) was collected, and the lungs were removed at different time intervals after OVA challenge to analyze inflammation, airway remodeling and airway hyperresponsiveness. Ovalbumin promoted leukocyte infiltration into BALF in a PAR2-dependent manner. ENMD1068 impaired eosinophil peroxidase (EPO) and myeloperoxidase (MPO) activity in the lung parenchyma into BALF and reduced the loss of dynamic pulmonary compliance, lung resistance in response to methacholine, mucus production, collagen deposition and chemokine (C-C motif) ligand 5 expression compared to those in OVA-challenged mice. We propose that proteases released after an allergen challenge may be crucial to the development of allergic asthma in mice, and PAR2 blockade may be useful as a new pharmacological approach for the treatment of airway allergic diseases

Ovarian Grafts 10 Days after Xenotransplantation: Folliculogenesis and Recovery of Viable Oocytes.

Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. However, cleavage and blastocyst rates of xenograft derived oocytes were lower than in control (P<0.01). Despite showing some functional modifications, the ovarian xenografts were able to support folliculogenesis and produce functional oocytes

Versican and Tumor-Associated Macrophages Promotes Tumor Progression and Metastasis in Canine and Murine Models of Breast Carcinoma

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2019-09-30T16:13:26Z No. of bitstreams: 1 Reis, C. D. Versican....pdf: 6212673 bytes, checksum: f0e5f7d54df5fde4549844031d7af599 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2019-09-30T16:50:18Z (GMT) No. of bitstreams: 1 Reis, C. D. Versican....pdf: 6212673 bytes, checksum: f0e5f7d54df5fde4549844031d7af599 (MD5)Made available in DSpace on 2019-09-30T16:50:18Z (GMT). No. of bitstreams: 1 Reis, C. D. Versican....pdf: 6212673 bytes, checksum: f0e5f7d54df5fde4549844031d7af599 (MD5) Previous issue date: 2019Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).Universidade Federal de Minas Gerais. Institute of Biological Sciences. Department of General Pathology. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Institute of Biological Sciences. Laboratory of Pulmonary Immunology and Mechanics. Department of Physiology and Biophysics. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal de Minas Gerais. Institute of Biological Sciences. Department of General Pathology. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Institute of Biological Sciences. Department of General Pathology. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Institute of Biological Sciences. Department of General Pathology. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Institute of Biological Sciences. Laboratory of Pulmonary Immunology and Mechanics. Department of Physiology and Biophysics. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Institute of Biological Sciences. Department of General Pathology. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Institute of Biological Sciences. Laboratory of Pulmonary Immunology and Mechanics. Department of Physiology and Biophysics. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Institute of Biological Sciences. Department of General Pathology. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Institute of Biological Sciences. Department of General Pathology. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Institute of Biological Sciences. Laboratory of Pulmonary Immunology and Mechanics. Department of Physiology and Biophysics. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Institute of Biological Sciences. Department of General Pathology. Belo Horizonte, MG, Brazil.Versican and tumor-associated macrophages (TAMs) are involved in growth and metastases in several cancers. Here, we investigated the potential role of versican, a matrix proteoglycan, and its correlation with TAMs infiltrates in different stages of two different breast cancer models: spontaneous canine mammary gland carcinomas and the murine 4T1 breast cancer model. The stromal versican expression was correlated with TAMs accumulation in tumors with an advanced stage from spontaneous canine mammary carcinoma samples. Versican expression in mice, identified in late stages of tumor progression, was associated to a high number of peri-tumoral infiltrating TAMs. Indeed, TAMs were related to a pro-inflammatory and pro-angiogenic state in the primary tumor. Furthermore, TAMs accumulation was related to versican expression in the lungs and an increased number of pulmonary metastatic nodules with pulmonary mechanical dysfunction, which was due to leukocyte influx in the airways and elevated growth factor levels in the microenvironment. Thus, we suggest that versican and TAMs as attractive targets for breast cancer therapy

Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice

Submitted by Nuzia Santos ([email protected]) on 2017-01-20T16:39:47Z No. of bitstreams: 1 ve_Nogueira_Denise_Multiple Exposures_CPqRR_2016.pdf: 7283772 bytes, checksum: 9287d516a1c1fa65a4453682a7bd231f (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2017-01-20T17:32:40Z (GMT) No. of bitstreams: 1 ve_Nogueira_Denise_Multiple Exposures_CPqRR_2016.pdf: 7283772 bytes, checksum: 9287d516a1c1fa65a4453682a7bd231f (MD5)Made available in DSpace on 2017-01-20T17:32:40Z (GMT). No. of bitstreams: 1 ve_Nogueira_Denise_Multiple Exposures_CPqRR_2016.pdf: 7283772 bytes, checksum: 9287d516a1c1fa65a4453682a7bd231f (MD5) Previous issue date: 2016Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Laboratorio de Imunologia e Genomica de Parasitos. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Laboratorio de Imunologia e Genomica de Parasitos. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Laboratorio de Imunologia e Genomica de Parasitos. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Laboratorio de Imunologia e Genomica de Parasitos. Belo Horizonte, MG, Brasil/Universidade Federal do Mato Grosso. Instituto de Biologia e Ciencia da Saude. Cuiabá, MT, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Laboratorio de Imunologia e Genomica de Parasitos. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Laboratorio de Imunologia e Genomica de Parasitos. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Laboratorio de Imunologia e Genomica de Parasitos. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Patologia Geral. Instituto de Ciencias Biologicas. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Fisiologia e Biofisica Laboratorio de Imunologia e Mecânica Pulmonar. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Fisiologia e Biofisica Laboratorio de Imunologia e Mecânica Pulmonar. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Patologia Geral. Instituto de Ciencias Biologicas. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, Brasil/University of Nottingham. United KingdomUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Laboratorio de Imunologia e Genomica de Parasitos. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Fisiologia e Biofisica Laboratorio de Imunologia e Mecânica Pulmonar. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Laboratorio de Imunologia e Genomica de Parasitos. Belo Horizonte, MG, Brasil/Universidade Federal do Mato Grosso. Instituto de Biologia e Ciencia da Saude. Cuiabá, MT, BrasilAscaris spp. infection affects 800 million people worldwide, and half of the world population is currently at risk of infection. Recurrent reinfection in humans is mostly due to the simplicity of the parasite life cycle, but the impact of multiple exposures to the biology of the infection and the consequences to the host's homeostasis are poorly understood. In this context, single and multiple exposures in mice were performed in order to characterize the parasitological, histopathological, tissue functional and immunological aspects of experimental larval ascariasis. The most important findings revealed that reinfected mice presented a significant reduction of parasite burden in the lung and an increase in the cellularity in the bronchoalveolar lavage (BAL) associated with a robust granulocytic pulmonary inflammation, leading to a severe impairment of respiratory function. Moreover, the multiple exposures to Ascaris elicited an increased number of circulating inflammatory cells as well as production of higher levels of systemic cytokines, mainly IL-4, IL-5, IL-6, IL-10, IL-17A and TNF-α when compared to single-infected animals. Taken together, our results suggest the intense pulmonary inflammation associated with a polarized systemic Th2/Th17 immune response are crucial to control larval migration after multiple exposures to Ascaris

Progression of folliculogenesis, morphometry and inflammatory cell numbers in xenografted and in control ovarian fragments.

<p>(a) Primordial, (b) primary, (c) secondary, (d) antral and (e) atretic follicles were noted 10 days after xenotransplantation. (g) Volumetric density (%) of ovarian pieces before and after xenotransplantation showing a higher incidence of follicular atresia and hyperemia (f; <i>P<</i>0.01) after this procedure. (h, i) As demonstrated using (h) NAG and (i) MPO assays in xenografted ovarian wet tissue, higher concentration of host-derived macrophages and neutrophils were observed (<i>P<</i>0.01). Black arrows = healthy follicles; red arrows = atretic follicles; white arrows = blood vessels; black arrowhead = hyperemia; asterisks = oocytes. Bars in a, b, c and e = 80 μm and d and f = 150 μm.</p

Primer sequences used for relative gene expression analysis by real-time polymerase chain reaction.

<p>Primer sequences used for relative gene expression analysis by real-time polymerase chain reaction.</p

Histopathological visualization of lesions caused by larval migration in the liver on the 4^th day post-infection and area of the lesions caused by larval migration.

<p>(A and B) Non-infected mouse; (C and D) Single-infected mouse with the presence of necrosis (arrowheads) and mild inflammatory infiltrate (arrows); (E and F) Reinfected mouse with presence of intense inflammatory infiltrate (arrows) and necrosis (*). Lower magnification Bar scale = 50 μm. Higher magnification Bar scale = 200 μm. (G) Area of lesion caused by larval migration in liver on the 4^th day post-infection. Mann-Whitney test was used to evaluate differences between groups.</p