Extension of Maximal Lifespan and High Bone Marrow Chimerism After Nonmyeloablative Syngeneic Transplantation of Bone Marrow From Young to Old Mice

The goal of this work was to determine the effect of nonablative syngeneic transplantation of young bone marrow (BM) to laboratory animals (mice) of advanced age upon maximum duration of their lifespan. To do this, transplantation of 100 million nucleated cells from BM of young syngeneic donors to an old nonablated animal was performed at the time when half of the population had already died. As a result, the maximum lifespan (MLS) increased by 28 ± 5%, and the survival time from the beginning of the experiment increased 2.8 ± 0.3-fold. The chimerism of the BM 6 months after the transplantation was 28%

Community clients of the agencies of social protection of Sverdlovsk region on social networks: formulation of research problem

The article presents the description of the author's research methodology, which allows during a particular research to characterize network communities by defining the socio-demographic characteristics of their members, the subject area of group discussion and the presence of information about the activities of specialists and a particular institution, the description and evaluation of specific services. The study helps to give more accurate description of the role and nature of the actions undertaken by the specialists of institutions interacting with these communities; to assess the information content of specific social groups concerning the existing links between the institution and the local community and other socially important organizations and structures.В статье представлено описание авторской исследовательской методики, позволяющей в реализации конкретного исследования дать характеристику сетевым сообществам, определив социально-демографические характеристики их членов; тематику группового обсуждения; наличие в контенте информации о деятельности специалистов и конкретного учреждения, описания и оценки конкретных услуг. Исследование позволит более четко охарактеризовать роль и характер действий специалистов учреждений, взаимодействующих с этими сообществами, дать оценку информационному контенту конкретных социальных групп на предмет существующих связей учреждения с местным сообществом и другими социально значимыми для горожан организациями и структурами

The molecular origin of the thiamin diphosphate-induced spectral bands of ThDP-dependent enzymes

New and previously published data on a variety of ThDP-dependent enzymes such as baker's yeast transketolase, yeast pyruvate decarboxylase and pyruvate dehydrogenase from pigeon breast muscle, bovine heart, bovine kidney, Neisseria meningitidis and E. coli show their spectral sensitivity to ThDP binding. Although ThDP-induced spectral changes are different for different enzymes, their universal origin is suggested as being caused by the intrinsic absorption of the pyrimidine ring of ThDP, bound in different tautomeric forms with different enzymes. Non-enzymatic models with pyrimidine-like compounds indicate that the specific protein environment of the aminopyrimidine ring of ThDP determines its tautomeric form and therefore the changeable features of the inducible effect. A polar environment causes the prevalence of the aminopyrimidine tautomeric form (short wavelength region is affected). For stabilization of the iminopyrimidine tautomeric form (both short- and long-wavelength regions are affected) two factors appear essential: (i) a nonpolar environment and (ii) a conservative carboxyl group of a specific glutamate residue interacting with the N1 atom of the aminopyrimidine ring. The two types of optical effect depend in a different way upon the pH, in full accordance with the hypothesis tested. From these studies it is concluded that the inducible optical rotation results from interaction of the aminopyrimidine ring with its asymmetric environment and is defined by the protonation state of N1 and the 4-nitroge

The role of Alu-derived RNAs in Alzheimer’s and other neurodegenerative conditions

International audienc

RESULTS OF STUDYING HAIRY VETCH (<i>VICIA VILLOSA </i> ROTH) IN MONO- AND BINARY AGROPHYTOCOENOSES AT SPRING PLANTING UNDER THE CONDITIONS OF THE NORTH-EAST OF THE RUSSIAN FEDERATION

Hairy vetch (Vicia villosa Roth), like common vetch (V. sativa L.), is one of the most widespread cultivated forage vetches. In Russia, hairy vetch is sown both in spring and winter planting seasons. V. villosa populations may contain spring, semi-spring, semi-winter and winter biotypes. Three hairy vetch varieties (Nezhnostebelnaya, Siverskaya 2 and Ukrainka) were studied in mono- and binary agrophytocoenosis with oat, rape and barley at spring sowing under the conditions of Leningrad Province of the Russian North-West in 2010-2012. Biotype composition of vetch variety populations was determined to identify adaptive biotypes. Some biological and agronomic characters were analysed. Comparative evaluation of hairy vetch variety populations was performed to test the role of agrophytocoenosis (and its phytocomponent) as a possible factor of biotype variability in vetch varieties and their biological and agronomic characters

Effect of Environmental Factors on Nuclear Organization and Transformation of Human B Lymphocytes

International audienc

Misdiagnosis of diabetic foot ulcer in patients with undiagnosed skin malignancies

A growing number of studies report dermal malignancies mimicking diabetic foot ulcers (DFUs). We reviewed clinical cases reporting malignant tumours misdiagnosed to be DFU aiming to identify factors contributing to misdiagnosis. We systematically searched in PubMed for clinical cases reporting on misdiagnosis of DFU in patients with cancer. A chi-square analysis was conducted to show the link between the incidence of initial DFU misdiagnosis and patient age, gender and wound duration. Lesions misdiagnosed to be DFU were subsequently diagnosed as melanoma (68% of the cases), Kaposi's sarcoma (14%), squamous cell carcinoma (11%), mantle cell lymphoma, and diffuse B-cell lymphoma (both by 4%). Older age (≥65 years) was associated with a significantly increased risk of malignancy masked as DFU (OR: 2.452; 95% CI: 1.132 to 5.312; P value = .019). The risk of such suspicion in older patients (age ≥ 65 years) was 145% higher than in younger patients (age &lt; 65 years). Clinicians should maintain a high level of awareness towards potentially malignant foot lesions in elderly patients with diabetes (age ≥ 65)