55 research outputs found
Inter-assay variability of next-generation sequencing-based gene panels
BACKGROUND: Tumor heterogeneity has been known to cause inter-assay discordance among next-generation sequencing (NGS) results. However, whether preclinical factors such as sample type, sample quality and analytical features of gene panel can affect the concordance between two different assays remains largely unexplored. METHODS: Replicate sets of DNA samples extracted from formalin-fixed paraffin-embedded tissues (FFPE) (nâ=â20) and fresh frozen (FF) tissues (nâ=â10) were herein analyzed using a tumor-only (TO) and paired tumor-normal (TN) gene panel in laboratories certified by the Clinical Laboratory Improvement Amendment. Reported variants from the TO and TN panels were then compared. Furthermore, additional FFPE samples were sequentially sliced from the same FFPE block and submitted to another TN panel assay. RESULTS: Substantial discordance (71.8%) was observed between the results of the two panels despite using identical DNA samples, with the discordance rate being significantly higher for FFPE samples (pâ<â0.05). Among the 99 variants reported only in the TO panel, 32.3% were consistent with germline variants, which were excluded in the TN panel, while 30.3% had an allele frequency of less than 5%, some of which were highly likely to be artificial calls. The comparison of two independent TN panel assay results from the same FFPE block also showed substantial discordance rate (55.3%). CONCLUSIONS: In the context of clinical settings, our comparative analysis revealed that inter-NGS assay discordance commonly occurred due to sample types and the different analytical features of each panel
ĂskĂŒdar mesireleri
Taha Toros ArĆivi, Dosya No: 63-Salacak-ĂskĂŒdarÄ°stanbul Kalkınma Ajansı (TR10/14/YEN/0033) Ä°stanbul Development Agency (TR10/14/YEN/0033
Structure near ++ threshold in the in-flight He reaction
To search for an S= -1 di-baryonic state which decays to , the reaction was studied at 1.0 GeV/.
Unobserved neutrons were kinematically identified from the missing mass
of the reaction in order to have a large
acceptance for the final state. The observed events,
distributed widely over the kinematically allowed region of the Dalitz plot,
establish that the major component comes from a three nucleon absorption
process. A concentration of events at a specific neutron kinetic energy was
observed in a region of low momentum transfer to the . To account
for the observed peak structure, the simplest S-wave pole was assumed to exist
in the reaction channel, having Breit-Wigner form in energy and with a Gaussian
form-factor. A minimum method was applied to deduce its mass
2355 (stat.) (syst.) MeV/c, and decay-width
110 (stat.) (syst.) MeV/c,
respectively. The form factor parameter 400 MeV/ implies that the
range of interaction is about 0.5Comment: 12pages, 8 figure
Search for the bound state via the in-flight reaction
In the J-PARC E15 experiment, a K â pp search was performed via the 3 He(K â , n ) reaction at 1.0 GeV/c . A forward-going neutron is detected by a neutron counter with 15 m flight length, and decay particles from K â pp are simultaneously measured by a cylindrical detector system that surrounds a liquid 3 He target system. In March and May, 2013, we carried out the first physics data-taking with 5Ă109 incident kaons on the 3 He target, and we have obtained a preliminary exclusive analysis result of 3 He(K â , Îp )n reaction
A search for the Kâpp bound state in the 3He(Kâin-flight, n) reaction at J-PARC
We have collected the first physics data of an experimental search for the simplest kaonic nuclear bound state, " K â pp", by the 3He( K â n) reaction at J-PARC. We confirmed that our spectrometer system works as designed and observed clear peak structure composed of the quasi-elastic K â " n " â K â n and the charge-exchange K â " p " â ÂŻ K 0 n reactions in the forward neutron spectrum
Pole position of measured in reactions
We measured a set of , , and
invariant mass spectra below and above the mass
threshold in -induced reactions on deuteron. We deduced the -wave
and scattering
amplitudes in the isospin 0 channel in the framework of a and
coupled channel. We find that a resonance pole corresponding to
is located at 1417.7(fitting
errors)(systematic errors) + (fitting
errors)(systematic errors)] MeV/, closer to the
mass threshold than the value determined by the Particle Data Group.Comment: 8 pages, 5 figure
A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)
Meeting abstrac
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