Genetic structure of the common sole Solea vulgaris at different geographic scales

The genetic structure of the flatfish #Solea vulgaris was investigated on several spatial scales and at the temporal level through analysis of electrophoretic variation at 8 to 12 polymorphic enzyme loci. No differentiation was apparent at the temporal scale. Some differentiation was detected at and above the regional scale. Isolation by distance was evidenced by the significant correlation between genetic and geographic distances, and by the consistency of the results of multiple-locus correspondence analysis with geographic sampling patterns. The analysis suggested that the geographic unit of population structure (i.e. a geographical area corresponding to a panmictic or nearly panmictic population) lies within a radius of the order of 100 km. The isolation-by-distance pattern in #S. vulgaris contrasted with the known genetic structures of other flatfish species of the northeastern Atlantic and Mediterranean in a way that may be related to the range of their respective temperature tolerances for eggs and larvae. (Résumé d'auteur

Evidence of high genetic connectivity for the longnose spurdog Squalus blainville in the Mediterranean Sea

Squalus blainville is one of the least studied Mediterranean shark species. Despite being intensively fished in several locations, biological knowledge is limited and no genetic structure information is available. This is the first study to examine the genetic structure of S. blainville in the Mediterranean Sea. Considering the high dispersal potential inferred for other squalid sharks, the hypothesis of panmixia was tested based on a 585 bp fragment of the mitochondrial DNA cytochrome c oxidase subunit I gene from 107 individuals and six nuclear microsatellite loci from 577 individuals. Samples were collected across the Ionian, Aegean and Libyan Seas and off the Balearic Islands. Twenty three additional sequences of Mediterranean and South African origin were retrieved from GenBank and included in the mitochondrial DNA analysis. The overall haplotype diversity was high, in contrast to the low nucleotide diversity. Low and non-significant pairwise ΦST and FST values along with a Bayesian cluster analysis suggested high connectivity with subsequent genetic homogeneity among the populations studied, and thus a high dispersal potential for S. blainville similar to other squalids. The historical demography of the species was also assessed, revealing a pattern of population expansion since the middle Pleistocene. These findings could be considered in species-specific conservation plans, although sampling over a larger spatial scale and more genetic markers are required to fully elucidate the genetic structure and dispersal potential of S. blainville

Strong population genetic structure and contrasting demographic histories for the small-spotted catshark (Scyliorhinus canicula) in the Mediterranean Sea

Coastal and demersal chondrichthyans, such as the small-spotted catshark, are expected to exhibit genetic differentiation in areas of complex geomorphology like the Mediterranean Basin because of their limited dispersal ability. To test this hypothesis, we used a fragment of the mitochondrial cytochrome c oxidase subunit I gene and 12 nuclear microsatellite loci in order to investigate the genetic structure and historical demography of this species, and to identify potential barriers to gene flow. Samples were collected from the Balearic Islands, the Algerian Basin, the Ionian Sea, the Corinthian Gulf and various locations across the Aegean Sea. Additional sequences from the Atlantic and the Levantine Basin retrieved from GenBank were included in the mitochondrial DNA analysis. Both mitochondrial and nuclear microsatellite DNA data revealed a strong genetic subdivision, mainly between the western and eastern Mediterranean, whereas the Levantine Basin shared haplotypes with both areas. The geographic isolation of the Mediterranean basins seems to enforce the population genetic differentiation of the species, with the deep sea acting as a strong barrier to its dispersal. Contrasting historical demographic patterns were also observed in different parts of the species' distribution, most notably a population growth trend in the western Mediterranean/ Atlantic area and a slight decreasing one in the Aegean Sea. The different effects of the Pleistocene glacial periods on the habitat availability may explain the contrasting demographic patterns observed. The current findings suggest that the smallspotted catshark exhibits several genetic stocks in the Mediterranean, although further study is needed

Design and evaluation of parallel hashing over large-scale data

High-performance analytical data processing systems often run on servers with large amounts of memory. A common data structure used in such environment is the hash tables. This paper focuses on investigating efficient parallel hash algorithms for processing large-scale data. Currently, hash tables on distributed architectures are accessed one key at a time by local or remote threads while shared-memory approaches focus on accessing a single table with multiple threads. A relatively straightforward “bulk-operation” approach seems to have been neglected by researchers. In this work, using such a method, we propose a high-level parallel hashing framework, Structured Parallel Hashing, targeting efficiently processing massive data on distributed memory. We present a theoretical analysis of the proposed method and describe the design of our hashing implementations. The evaluation reveals a very interesting result - the proposed straightforward method can vastly outperform distributed hashing methods and can even offer performance comparable with approaches based on shared memory supercomputers which use specialized hardware predicates. Moreover, we characterize the performance of our hash implementations through extensive experiments, thereby allowing system developers to make a more informed choice for their high-performance applications

Genetic vs community diversity patterns of macrobenthic species: preliminary results from the lagoonal ecosystem

1 - The use of molecular data derived from multispecies assemblages in order to test ecological theory has only recently been introduced in the scientific literature.2 - As a first step, we compared patterns of abiotic environment, polychaeta distribution and their genetic diversity in five lagoon ecosystems in Greece. Our results confirm the hypothesis that higher genetic diversity is expected in the populations of the species occurring in the transitional waters rather than of those occurring in the marine environment.3 - Patterns derived from the polychaete community level and from the mitochondrial DNA (16S rRNA) obtained from Nephtys hombergii and Hediste diversicolor showed convergence, indicating the potential use of molecular matrices as surrogates in community analysis.4 - Finally, the high correlation between the genetic diversity pattern of H. diversicolor and the phosphorus concentration in the sediments may imply the broadening of the hierarchic-response-tostress hypothesis towards lower than species level

A hybrid framework for nonlinear dynamic simulations including full-field optical measurements and image decomposition algorithms

Innovative designs of transport vehicles need to be validated in order to demonstrate reliability and provide confidence. It is normal practice to study the mechanical response of the structural elements by comparing numerical results obtained from finite element simulation models with results obtained from experiment. In this frame, the use of wholefield optical techniques has been proven successful in the validation of deformation, strain, or vibration modes. The strength of full-field optical techniques is that the entire displacement field can be acquired. The objective of this article is to integrate full-field optical measurement methodologies with state-of-the-art computational simulation techniques for nonlinear transient dynamic events. In this frame, composite car bonnet frame structures of dimensions about 1.8 m 30.8 m are considered. They have been tested in low-velocity mass-drop impact loading with impact energies ranging from 20 to 200 J. In parallel, simulation models of the car bonnet frame have been developed using layered shell elements. The Zernike shape descriptor approach was used to decompose numerical and experimental data into moments for comparison purposes. A very good agreement between numerical and experimental results was observed. Therefore, integration of numerical analysis with full-field optical measurements along with sophisticated comparison techniques can increase design reliability

A genetic linkage map of the hermaphrodite teleost fish Sparus aurata L.

The gilthead sea bream (Sparus aurata L.) is a marine fish of great importance for fisheries and aquaculture. It has also a peculiar sex-determination system, being a protandrous hermaphrodite. Here we report the construction of a first-generation genetic linkage map for S. aurata, based on 204 microsatellite markers. Twenty-six linkage groups (LG) were found. The total map length was 1241.9 cM. The ratio between sex-specific map lengths was 1:1.2 (male:female). Comparison with a preliminary radiation hybrid (RH) map reveals a good concordance, as all markers located in a single LG are located in a single RH group, except for Ad-25 and CId-31. Comparison with the Tetraodon nigroviridis genome revealed a considerable number of evolutionary conserved regions (ECRs) between the two species. The mean size of ECRs was 182 bp (sequence identity 60–90%). Forty-one ECRs have a known chromosomal location in the pufferfish genome. Despite the limited number of anchoring points, significant syntenic relationships were found. The linkage map presented here provides a robust comparative framework for QTL analysis in S. aurata and is a step toward the identification of genetic loci involved both in the determination of economically important traits and in the individual timing of sex reversal