Use of somatic cell count as an indicator of colostrum quality

The impact of cow mammary gland diseases on the quality of colostrum is not conclusively defined; research results are conflicting. However, it is widely believed that mastitis lowers the level of immunoglobulins and the quality of the colostrum produced. Therefore, the aim of this study was to determine the influence of somatic cell counts (SCC) on the colostrum immuno-stimulating and chemical components. The experiment was conducted on an experimental organic dairy farm in which a herd of approximately 250 cows was kept in a freestall housing system, with the average performance exceeding 6,000 kg of milk per lactation. Colostrum and milk samples were taken individually from each cow seven times during the experiment: from the first to second day after calving–twice per day, and from the third to fifth day after calving–once per day. Therefore, after preliminary analyses, the cows were divided into two groups based on the cytological quality of their colostrum at the first collection: 1. SCC �400,000 cells/ml (good quality colostrum; GCC– 18 cows), 2. SCC � 400,000 cells/ml (low quality colostrum; LCC– 22 cows). The study found almost double the concentration of immunoglobulins and essential fatty acids in first milking colostrum in the GCC group than in colostrum from the LCC group. In addition, an increase in the concentration of lysozyme in first milking colostrum was associated with a decrease in the concentration of immunoglobulins. In addition, the increase in the level of lysozyme was associated with a decrease in the concentration of immunoglobulins. In conclusion, the SCC of first milking colostrum can be used as an indicator of colostrum quality

Age of cows, as a factor shaping the level of immunostimulating properties of colostrum

A characteristic feature of the organic system of production is the lower productivity of cows. The aim of the study was to determine the effect of cow age and collecting number on the level of immunostimulating colostrum components in an organic farm. From the basic herd specializing in organic milk production, 40 cows of the Polish Holstein-Friesian Black and White breed were selected: 10 primiparous, 10 cows in 2nd lactation, 10 cows in 4th lactation and 10 cows in 5th lactation. The colostrum samples were collected according to the following scheme: the first one maximum of two hours after calving, the second on the same day and the third and fourth on the following day. For three consecutive days samples were taken once a day (7 colostrum samples from each cow). Statistically significant differences in the level of bioactive components of colostrum with immunostimulating properties has been shown due to the time of intake from calving and significant differences in the level of these components due to the age of cows. Multiparous cows synthetized colostrum with a higher content of total protein, casein and non-fat dry matter than the primiparous. Variability of the immunoglobulin content of colostrum obtained in 1st and in the 2nd collecting after calving was higher in multiparous cows than in primiparous cows. Furthermore, it has been shown that there was a clear correlation between the quality of colostrum and the age of cows. In conclusion, a high impact of the interaction of age of cows x collecting number on the development of colostrum stimulating ingredients in the organic production system has been demonstrated

Interaction between the level of immunoglobulins and number of somatic cells as a factor shaping the immunomodulating properties of colostrum

The aim of this study was to investigate the association between immunoglobulins and SCC as a factor in shaping the content of the immunostimulatory components of colostrum. Seventy-eight multiparous Polish Holstein–Friesian cows were selected for the experiment. Colostrum samples were collected immediately after calving (up to a max. of 2 h). The cows were divided into groups according to the following levels: Immunoglobulins (IG class)—(IG1) over 50 g/L, (IG2) up to 50 g/L; SCC class—(SCC1) up to 400 000/ml, (SCC2) 400–800 000/ml, (SCC3) over 800 000/ml. Colostrum assigned to the IG1 SCC1 group had a statistically significant higher (p ≤ 0.01) concentration of both whey proteins and fatty acids compared to the IG1 SCC2 and SCC3 groups. The concentration of IgG, IgM, and IgA was shown to be higher in IG1 SCC1 than IG2 SCC3 by 226%, 149%, and 115%, respectively. The concentration of lactoferrin was shown to be higher in IG1 SCC1 than IG2 SCC3 by 149%. The determination of colostrum quality based on the concentration of immunoglobulins in the colostrum may not be sufficient because serum IgG concentrations at birth show a linear increase relative to colostrum SCC. A breakdown of colostrum into quality classes, taking into account the level of SCC, should therefore be introduced

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

On the Efficiency of Population-Based Optimization in Finding Best Parameters for RGB-D Visual Odometry

Visual odometry estimates the transformations between consecutive frames of a video stream in order to recover the camera’s trajectory. As this approach does not require to build a map of the observed environment, it is fast and simple to implement. In the last decade RGBD cameras proliferated in roboTIcs, being also the sensors of choice for many practical visual odometry systems. Although RGB-D cameras provide readily available depth images, that greatly simplify the frame-to-frame transformations computaTIon, the number of numerical parameters that have to be set properly in a visual odometry system to obtain an accurate trajectory estimate remains high. Whereas seƫng them by hand is certainly possible, it is a tedious try-and-error task. Therefore, in this article we make an assessment of two population-based approaches to parameter opTImizaTIon, that are for long time applied in various areas of robotics, as means to find best parameters of a simple RGB-D visual odometry system. The optimization algorithms investigated here are particle swarm optimization and an evolutionary algorithm variant. We focus on the optimization methods themselves, rather than on the visual odometry algorithm, seeking an efficient procedure to find parameters that minimize the estimated trajectory errors. From the experimental results we draw conclusions as to both the efficiency of the optimization methods, and the role of particular parameters in the visual odometry system

Polymorphism of Genes and Their Impact on Beef Quality

The single-nucleotide polymorphism (SNP) form of genes is a valuable source of information regarding their suitability for use as specific markers of desirable traits in beef cattle breeding. For several decades, breeding work focused on improving production efficiency through optimizing the feed conversion ratio and improving daily gains and meat quality. Many research teams previously undertook research work on single-nucleotide polymorphism in myostatin (MSTN), thyroglobulin (TG), calpain (CAPN), and calpastatin (CAST) proteins. The literature review focuses on the most frequently addressed issues concerning these genes in beef cattle production and points to a number of relevant studies on the genes’ polymorphic forms. The four genes presented are worth considering during breeding work as a set of genes that can positively influence productivity and production quality

Organic Milk Production and Dairy Farming Constraints and Prospects under the Laws of the European Union

In recent years, there has been rapid development in organic farming. When choosing organic livestock products, consumers are guided by the conviction that animals are provided with the highest welfare standards and access to pasture. The purpose of this article was to trace the principles of organic farming prevailing in the EU with regard to milk production and cattle breeding. The principles of organic production are universal and their application is not limited to certified farms. Organic certification is intended to assure the consumer of the quality and method of production. Due to additional requirements imposed by law, organic cows are usually kept in better welfare conditions compared to conventional cattle, but this is not the rule. The altered taste and texture of organic milk and its products compared to conventional products mainly depends on the presence of pasture greens in the cows’ diet. Therefore, milk from conventionally kept, pasture-grazed cows may have similar characteristics and composition. Organic farms tend to have lower milk yields compared to conventional farms due to the lower consumption of concentrate feed. In the future, it is expected that the proportion of land that is unsuitable for the production of crops for human consumption will increasingly be used for cow grazing

The Effect of Parity on the Quality of Colostrum of Holstein Dairy Cows in the Organic Production System

A special feature of organic production systems is the cows’ significantly lower productivity, which is adapted to environmental conditions. The quantity and quality of colostrum is negatively correlated, high amounts of colostrum are associated with low amounts of immunoglobulins. Cows’ parity is also a modulating factor, and studies have shown an increase in colostral IgG corresponds with increasing parity. This study’s aim was to determine the effect of cows’ parity on colostrum quality, in an organic farm setting. From a basic organic herd of dairy cattle, 40 Polish Holstein–Friesian cows were selected: 10 cows each of primiparous, second lactation, fourth lactation, and fifth lactation. Colostrum and transition milk samples were taken from each cow seven times: twice daily on the 1st and 2nd days after calving (every 12 h), and once daily on the 3rd to 5th days. Multiparous cows’ colostrum had higher levels of total proteins, casein, and non-fat dry matter, versus primiparous. Only cows in the second and fourth lactations produced very good quality colostrum (with immunoglobulins over 50 g/L), meeting standards for immunoglobulin concentration. In conclusion, the production of very good quality colostrum is limited during the first lactation, which may suggest the mammary gland is poorly developed, and, thus, immunoglobulin transport is limited. Variability in the colostrum’s immunoglobulin content from first and second collections post-calving was higher in multiparous versus primiparous cows. Therefore, it should be good practice to freeze colostrum from multiparous cows in case of poor-quality primiparous colostrum

Comparison of Enzyme Activity in Order to Describe the Metabolic Profile of Dairy Cows during Early Lactation

Enzymatic diagnostics have practical applications in diseases of the liver, heart, pancreas, muscles, blood, and neoplastic diseases. This study aimed to compare enzyme activity to describe dairy cows’ metabolism during early lactation. Based on their general health symptoms, the cows were assigned to one of three groups: acidotic, healthy and ketotic. Samples of milk, blood and rumen fluid were collected at 12 ± 5 days postpartum. Ketotic cows were characterized by the highest malondialdehyde (MDA, 76.098 nM/mL), glutathione reductase (GluRed, 109.852 U/L), superoxide dismutase (SOD, 294.22 U/L) and gamma-glutamyltranspeptidase (GGTP, 71.175 U/L) activity. In comparing ketotic and acidotic cows, MDA, GluRed, SOD and GGTP activity were higher by a factor of almost: 1.85, 1.89, 0.79 and 2.50, respectively. Acidotic cows were characterized by the highest aspartate aminotransferase activity (AspAT, 125.914 U/L). In comparing acidotic and ketotic cows, AspAT activity was higher by a factor of almost 1.90. The use of enzymatic markers could limit the frequency of sampling for laboratory analyses and may result in a faster diagnosis of metabolic disorders. AspAT activity in blood serum seems to be a good indicator of acidosis; GGTP may participate in the pathogenesis of ketosis