Establishing a Community-Academic Partnership to Investigate the Sociopolitical Context of Oral Care Among Refugees Resettled in Richmond, Virginia

Establishing a Community-Academic Partnership to Investigate the Sociopolitical Context of Oral Care Among Refugees Resettled in Richmond, Virginia Tatiana Brown, Depts. of Anthropology, Medical Humanities, & Mathematics, with Dr. Dina Garcia, Dept. of Health Behavior and Policy This study has established a community-academic partnership between four organizations and VCU Health Behavior and Policy’s Kalpulli Research Team to investigate the sociopolitical context of oral health needs among refugees resettling in Richmond, Virginia. In the year 2018, 1,689 refugees resettled in the state of Virginia; 47.7 percent of this population reported oral health needs during their initial health assessment screening. Despite the oral health needs present within this population, little is known about the facilitators and barriers to dental care access for this population post-resettlement. Creating a partnership with two local resettlement agencies (e.g., the International Rescue Committee, Commonwealth Catholic Charities), the Department of Social Services (DSS), and VA Department of Health’s Newcomer (Refugee) Health Program, in addition to having a transdisciplinary research team, enable the development of a holistic representation within, and of, the refugee community. The use of semi-structured interviews is the strongest approach to build the research team’s understanding of community perceptions towards access to oral health care, refugee health workers’ identified barriers to equitable care, and providers’ attitudes towards treating the refugee and Medicaid population. Community partners are key in shaping study recruitment, determining which interview questions will be most salient, and creating an effective intervention from the results. This partnership plans to create two collaborative interventions: a process map to help refugees better navigate establishing care with local providers and “oral care cards” in the top three refugee languages (Arabic, Farsi/Dhari, and Swahili) to be distributed through dental providers in the area.https://scholarscompass.vcu.edu/uresposters/1356/thumbnail.jp

Stakeholder Perceptions of Health Needs in Refugee Populations in the Greater Richmond Area

Stakeholder Perceptions of Health Needs in Refugee Populations in the Greater Richmond Area Ashley Koo, Depts. of Medical Anthropology and Chemistry, Matt Tessama, and Tatiana Brown, with Dr. Dina Garcia, Dept. of Health Behavior and Policy A refugee is a person outside of the country of his or her nationality who is unable or unwilling to return to that country because of persecution or a well-founded fear of persecution based on her race, religion, nationality, or membership in a certain social group. The United Nations High Commissioner on Refugees estimates that in 2018, approximately 22,500 refugees were resettled in the U.S. with 1,805 arrived in Virginia. Many of these refugees face multiple health challenges post-resettlement, particularly in oral health. Although these oral health needs are a recognized concern, there is very little information to support what the specific facilitators and barriers to dental care access is for this population. The long-term objective of this study is to identify the largest gaps in healthcare access for refugee populations, and plan an intervention to bridge these gaps through local clinics. The health and oral health needs of refugees post-resettlement can be determined through one-on-one semi-structured interviews with health liaisons. The participants will be recruited from a list of organizations involved in refugee resettlement and employment, including the Virginia Department of Health, Commonwealth Catholic Charities, ReEstablish Richmond and the International Rescue Committee. The interviews will allow stakeholders to share their experience serving the refugee community, their perspective on health needs that are present in this population and ideas on how to address these needs. The interviews will be audiotaped and then transcribed via research participants and stored in the password-protected MAXQDA software. There exists a link between dental/ oral health and overall health: untreated dental cavities can lead to cardiovascular issues and can be fatal. Intervention within the community is needed to increase healthcare access; analyzing stakeholder perspectives will allow greater understanding of what inequities exist for refugee populations in the Greater Richmond area.https://scholarscompass.vcu.edu/uresposters/1374/thumbnail.jp

A Clinician\u27s Guide to Next Generation Imaging in Patients With Advanced Prostate Cancer (RADAR III).

PURPOSE: The advanced prostate cancer therapeutic landscape has changed dramatically in the last several years, resulting in improved overall survival of patients with castration naïve and castration resistant disease. The evolution and development of novel next generation imaging techniques will affect diagnostic and therapeutic decision making. Clinicians must navigate when and which next generation imaging techniques to use and how to adjust treatment strategies based on the results, often in the absence of correlative therapeutic data. Therefore, guidance is needed based on best available information and current clinical experience. MATERIALS AND METHODS: The RADAR (Radiographic Assessments for Detection of Advanced Recurrence) III Group convened to offer guidance on the use of next generation imaging to stage prostate cancer based on available data and clinical experience. The group also discussed the potential impact of next generation imaging on treatment options based on earlier detection of disease. RESULTS: The group unanimously agreed that progression to metastatic disease is a seminal event for patient treatment. Next generation imaging techniques are able to detect previously undetectable metastases, which could redefine the phases of prostate cancer progression. Thus, earlier systemic or locally directed treatment may positively alter patient outcomes. CONCLUSIONS: The RADAR III Group recommends next generation imaging techniques in select patients in whom disease progression is suspected based on laboratory (biomarker) values, comorbidities and symptoms. Currently 18F-fluciclovine and 68Ga prostate specific membrane antigen positron emission tomography/computerized tomography are the next generation imaging agents with a favorable combination of availability, specificity and sensitivity. There is ongoing research of additional next generation imaging technologies, which may offer improved diagnostic accuracy and therapeutic options. As next generation imaging techniques evolve and presumably result in improved global accessibility, clinician ability to detect micrometastases may be enhanced for decision making and patient outcomes

Isolation and culture of murine bone marrow-derived macrophages for nitric oxide and redox biology

Macrophages are mononuclear phagocytes derived from haematopoietic progenitors that are widely distributed throughout the body. These cells participate in both innate and adaptive immune responses and lie central to the processes of inflammation, development, and homeostasis. Macrophage physiology varies depending on the environment in which they reside and they exhibit rapid functional adaption in response to external stimuli. To study macrophages in vitro, cells are typically cultured ex vivo from the peritoneum or alveoli, or differentiated from myeloid bone marrow progenitor cells to form bone marrow-derived macrophages (BMDMs). BMDMs represent an efficient and cost-effective means of studying macrophage biology. However, the inherent sensitivity of macrophages to biochemical stimuli (such as cytokines, metabolic intermediates, and RNS/ROS) makes it imperative to control experimental conditions rigorously. Therefore, the aim of this study was to establish an optimised and standardised method for the isolation and culture of BMDMs. We used classically activated macrophages isolated from WT and nitric oxide (NO)-deficient mice to develop a standardised culture method, whereby the constituents of the culture media are defined. We then methodically compared our standardised protocol to the most commonly used method of BMDM culture to establish an optimal protocol for the study of nitric oxide (NO)-redox biology and immunometabolism in vitro. [Abstract copyright: Copyright © 2020. Published by Elsevier Inc.

Pan-Cancer Analysis of lncRNA Regulation Supports Their Targeting of Cancer Genes in Each Tumor Context

Long noncoding RNAs (lncRNAs) are commonly dys-regulated in tumors, but only a handful are known toplay pathophysiological roles in cancer. We inferredlncRNAs that dysregulate cancer pathways, onco-genes, and tumor suppressors (cancer genes) bymodeling their effects on the activity of transcriptionfactors, RNA-binding proteins, and microRNAs in5,185 TCGA tumors and 1,019 ENCODE assays.Our predictions included hundreds of candidateonco- and tumor-suppressor lncRNAs (cancerlncRNAs) whose somatic alterations account for thedysregulation of dozens of cancer genes and path-ways in each of 14 tumor contexts. To demonstrateproof of concept, we showed that perturbations tar-geting OIP5-AS1 (an inferred tumor suppressor) andTUG1 and WT1-AS (inferred onco-lncRNAs) dysre-gulated cancer genes and altered proliferation ofbreast and gynecologic cancer cells. Our analysis in-dicates that, although most lncRNAs are dysregu-lated in a tumor-specific manner, some, includingOIP5-AS1, TUG1, NEAT1, MEG3, and TSIX, synergis-tically dysregulate cancer pathways in multiple tumorcontexts

Pan-cancer Alterations of the MYC Oncogene and Its Proximal Network across the Cancer Genome Atlas

Although theMYConcogene has been implicated incancer, a systematic assessment of alterations ofMYC, related transcription factors, and co-regulatoryproteins, forming the proximal MYC network (PMN),across human cancers is lacking. Using computa-tional approaches, we define genomic and proteo-mic features associated with MYC and the PMNacross the 33 cancers of The Cancer Genome Atlas.Pan-cancer, 28% of all samples had at least one ofthe MYC paralogs amplified. In contrast, the MYCantagonists MGA and MNT were the most frequentlymutated or deleted members, proposing a roleas tumor suppressors.MYCalterations were mutu-ally exclusive withPIK3CA,PTEN,APC,orBRAFalterations, suggesting that MYC is a distinct onco-genic driver. Expression analysis revealed MYC-associated pathways in tumor subtypes, such asimmune response and growth factor signaling; chro-matin, translation, and DNA replication/repair wereconserved pan-cancer. This analysis reveals insightsinto MYC biology and is a reference for biomarkersand therapeutics for cancers with alterations ofMYC or the PMN

Real-Time Imaging and Quantification of Amyloid-β Peptide Aggregates by Novel Quantum-Dot Nanoprobes

Background: Protein aggregation plays a major role in the pathogenesis of neurodegenerative disorders, such as Alzheimer’s disease. However, direct real-time imaging of protein aggregation, including oligomerization and fibrillization, has never been achieved. Here we demonstrate the preparation of fluorescent semiconductor nanocrystal (quantum dot; QD)-labeled amyloid-b peptide (QDAb) and its advanced applications. Methodology/Principal Findings: The QDAb construct retained Ab oligomer-forming ability, and the sizes of these oligomers could be estimated from the relative fluorescence intensities of the imaged spots. Both QDAb coaggregation with intact Ab42 and insertion into fibrils were detected by fluorescence microscopy. The coaggregation process was observed by real-time 3D imaging using slit-scanning confocal microscopy, which showed a typical sigmoid curve with 1.5 h in the lag-time and 12 h until saturation. Inhibition of coaggregation using an anti-Ab antibody can be observed as 3D images on a microscopic scale. Microglia ingested monomeric QDAb more significantly than oligomeric QDAb, and the ingested QDAb was mainly accumulated in the lysosome. Conclusions/Significance: These data demonstrate that QDAb is a novel nanoprobe for studying Ab oligomerization an

Genomic, Pathway Network, and Immunologic Features Distinguishing Squamous Carcinomas

This integrated, multiplatform PanCancer Atlas study co-mapped and identified distinguishing molecular features of squamous cell carcinomas (SCCs) from five sites associated with smokin

Spatial Organization and Molecular Correlation of Tumor-Infiltrating Lymphocytes Using Deep Learning on Pathology Images

Beyond sample curation and basic pathologic characterization, the digitized H&E-stained images of TCGA samples remain underutilized. To highlight this resource, we present mappings of tumorinfiltrating lymphocytes (TILs) based on H&E images from 13 TCGA tumor types. These TIL maps are derived through computational staining using a convolutional neural network trained to classify patches of images. Affinity propagation revealed local spatial structure in TIL patterns and correlation with overall survival. TIL map structural patterns were grouped using standard histopathological parameters. These patterns are enriched in particular T cell subpopulations derived from molecular measures. TIL densities and spatial structure were differentially enriched among tumor types, immune subtypes, and tumor molecular subtypes, implying that spatial infiltrate state could reflect particular tumor cell aberration states. Obtaining spatial lymphocytic patterns linked to the rich genomic characterization of TCGA samples demonstrates one use for the TCGA image archives with insights into the tumor-immune microenvironment