110 research outputs found
The features of chemo-mechanical activation technology of polymer composite materials production
The paper deals with the study of the processes of obtaining polymer composites by chemo-mechanical activation technology (CMA-technology). The methods of research and testing of composite materials based on polytetrafluoroethylene (PTFE) and carbon fibers are analyzed and generalized. The influence of CMA technology on the structure and properties of PTFE composite was determined
The origin of paramagnetic magnetization in field-cooled YBa2Cu3O7 films
Temperature dependences of the magnetic moment have been measured in
YBa_2Cu_3O_{7-\delta} thin films over a wide magnetic field range (5 <= H <=
10^4 Oe). In these films a paramagnetic signal known as the paramagnetic
Meissner effect has been observed. The experimental data in the films, which
have strong pinning and high critical current densities (J_c ~ 2 \times 10^6
A/cm^2 at 77 K), are quantitatively shown to be highly consistent with the
theoretical model proposed by Koshelev and Larkin [Phys. Rev. B 52, 13559
(1995)]. This finding indicates that the origin of the paramagnetic effect is
ultimately associated with nucleation and inhomogeneous spatial redistribution
of magnetic vortices in a sample which is cooled down in a magnetic field. It
is also shown that the distribution of vortices is extremely sensitive to the
interplay of film properties and the real experimental conditions of the
measurements.Comment: RevTex, 8 figure
High shock release in ultrafast laser irradiated metals: Scenario for material ejection
We present one-dimensional numerical simulations describing the behavior of
solid matter exposed to subpicosecond near infrared pulsed laser radiation. We
point out to the role of strong isochoric heating as a mechanism for producing
highly non-equilibrium thermodynamic states. In the case of metals, the
conditions of material ejection from the surface are discussed in a
hydrodynamic context, allowing correlation of the thermodynamic features with
ablation mechanisms. A convenient synthetic representation of the thermodynamic
processes is presented, emphasizing different competitive pathways of material
ejection. Based on the study of the relaxation and cooling processes which
constrain the system to follow original thermodynamic paths, we establish that
the metal surface can exhibit several kinds of phase evolution which can result
in phase explosion or fragmentation. An estimation of the amount of material
exceeding the specific energy required for melting is reported for copper and
aluminum and a theoretical value of the limit-size of the recast material after
ultrashort laser irradiation is determined. Ablation by mechanical
fragmentation is also analysed and compared to experimental data for aluminum
subjected to high tensile pressures and ultrafast loading rates. Spallation is
expected to occur at the rear surface of the aluminum foils and a comparison
with simulation results can determine a spall strength value related to high
strain rates
Intragenic DNA methylation: implications of this epigenetic mechanism for cancer research
Epigenetics is the study of all mechanisms that regulate gene transcription and genome stability that are maintained throughout the cell division, but do not include the DNA sequence itself. The best-studied epigenetic mechanism to date is DNA methylation, where methyl groups are added to the cytosine base within cytosine–guanine dinucleotides (CpG sites). CpGs are frequently clustered in high density (CpG islands (CGIs)) at the promoter of over half of all genes. Current knowledge of transcriptional regulation by DNA methylation centres on its role at the promoter where unmethylated CGIs are present at most actively transcribed genes, whereas hypermethylation of the promoter results in gene repression. Over the last 5 years, research has gradually incorporated a broader understanding that methylation patterns across the gene (so-called intragenic or gene body methylation) may have a role in transcriptional regulation and efficiency. Numerous genome-wide DNA methylation profiling studies now support this notion, although whether DNA methylation patterns are a cause or consequence of other regulatory mechanisms is not yet clear. This review will examine the evidence for the function of intragenic methylation in gene transcription, and discuss the significance of this in carcinogenesis and for the future use of therapies targeted against DNA methylation
Microwave response of single crystal YBa₂Cu₃O₇–δ films as a probe for pairing symmetry
Temperature dependences of the microwave surface impedance, Zs(T), are measured in the
c-axis oriented single-crystal high-Tc superconducting cuprate YBa₂Cu₃O₇₋δ (YBCO) thin films
deposited by the off-axis dc magnetron sputtering onto CeO₂-buffered single-crystal sapphire
substrates (film thickness is d ≈ 150, 300, 480 nm). Measurements are performed by a use of the
coplanar resonator as well as the end-plate cylindrical cavity resonator techniques at a number of
several discrete frequencies within the range of 5–134 GHz. The measurements have revealed
unexpected peculiarities on the Zs(T)-dependences for the most perfect films under study. The
peculiarities appear to be most strongly pronounced on the temperature dependences of the film
surface resistance Rs(T) = Re {Zs(T)}. The most important features of the unusual surface
resistance behavior are: (i) the temperature dependence Rs(T) of YBCO films under study at low
temperatures obeys the exponential law: Rs(T) = Rres + R₀ exp [–∆s/T] with the small energy gap
∆s (∆s 0.5Tc at f = 5 GHz); (ii) the most perfect films reveal a distinct two-peak structure of the
Rs(T) dependence with peaks positioned at 27–30 K and 48–51 K, while such peaks are not
observed in less perfect films. The peaks are mostly pronounced at moderate (e.g. 34 GHz)
frequencies and gradually disappear both at higher and lower frequencies, while their temperature
positions remain unchanged. These features of perfect single-crystalline YBCO films are believed
to reveal their intrinsic electron properties. Taking into account the possibility of a mixed
(s+id)-type pairing symmetry as well as a significant role of extended out-of-plane crystal defects
(such as dislocation lines or twin planes) in Bogolyubov’s quasiparticle scattering within the most
perfect YBCO films, one can suggest a consistent explanation for the anomalies observed in the
Zs(T) behavior
Mechanisms of limitation and nature of field dependence of critical current in HTS epitaxial YBaCuO films
Magnetic field and temperature dependencies of the critical current density, J/sub c/(H/spl par/c, T) were measured by SQUID-magnetometry, ac magnetic susceptibility, and dc transport current techniques in the single-crystalline epitaxially-grown by off-axis dc magnetron sputtering YBa/sub 2/Cu/sub 3/O/sub 7-/spl delta// (YBCO) films with J/sub c/(H/spl par/c, 77 K) /spl ges/ 2 /spl middot/ 10/sup 6/ A/cm/sup 2/. The mechanism of vortex depinning from growth-induced linear defects, i.e., out-of-plane edge dislocations in low-angle tilt domain boundaries, is shown to describe quantitatively measured J/sub c/(H/spl par/c, T). The developed model takes into account a statistical distribution of the dislocation domain boundaries ordered in a network as well as the interdislocation spacing within boundaries. Actual structural features of YBCO film known from HREM data turn out to be extracted from J/sub c/(H/spl par/c, T)-curves by a fitting procedure within the proposed model
Transient optical response of ultrafast nonequilibrium excited metals: Effects of electron-electron contribution to collisional absorption
Approaching energy coupling in laser-irradiated metals, we point out the role
of electron-electron collision as an efficient control factor for ultrafast
optical absorption. The high degree of laser-induced electron-ion
nonequilibrium drives a complex absorption pattern with consequences on the
transient optical properties. Consequently, high electronic temperatures
determine largely the collision frequency and establish a transition between
absorptive regimes in solid and plasma phases. In particular, taking into
account umklapp electron-electron collisions, we performed hydrodynamic
simulations of the laser-matter interaction to calculate laser energy
deposition during the electron-ion nonequilibrium stage and subsequent matter
transformation phases. We observe strong correlations between optical and
thermodynamic properties according to the experimental situations. A suitable
connection between solid and plasma regimes is chosen in accordance with models
that describe the behavior in extreme, asymptotic regimes. The proposed
approach describes as well situations encountered in pump-probe types of
experiments, where the state of matter is probed after initial excitation.
Comparison with experimental measurements shows simulation results which are
sufficiently accurate to interpret the observed material behavior. A numerical
probe is proposed to analyze the transient optical properties of matter exposed
to ultrashort pulsed laser irradiation at moderate and high intensities.
Various thermodynamic states are assigned to the observed optical variation.
Qualitative indications of the amount of energy coupled in the irradiated
targets are obtained.
Keywords: ultrafast absorption ; umklapp electron-electron collision ;
collisional absorption ; laser-matter interactio
Global profiling of histone and DNA methylation reveals epigenetic-based regulation of gene expression during epithelial to mesenchymal transition in prostate cells
<p>Abstract</p> <p>Background</p> <p>Previously we reported extensive gene expression reprogramming during epithelial to mesenchymal transition (EMT) of primary prostate cells. Here we investigated the hypothesis that specific histone and DNA methylations are involved in coordination of gene expression during EMT.</p> <p>Results</p> <p>Genome-wide profiling of histone methylations (H3K4me3 and H3K27me3) and DNA methylation (DNAMe) was applied to three cell lines at different stages of a stepwise prostate cell model involving EMT and subsequent accumulation of malignant features. Integrated analyses of epigenetic promoter modifications and gene expression changes revealed strong correlations between the dynamic changes of histone methylations and gene expression. DNA methylation was weaker associated with global gene repression, but strongly correlated to gene silencing when genes co-modified by H3K4me3 were excluded. For genes labeled with multiple epigenetic marks in their promoters, the level of transcription was associated with the net signal intensity of the activating mark H3K4me3 minus the repressive marks H3K27me3 or DNAMe, indicating that the effect on gene expression of bivalent marks (H3K4/K27me3 or H3K4me3/DNAMe) depends on relative modification intensities. Sets of genes, including epithelial cell junction and EMT associated fibroblast growth factor receptor genes, showed corresponding changes concerning epigenetic modifications and gene expression during EMT.</p> <p>Conclusions</p> <p>This work presents the first blueprint of epigenetic modifications in an epithelial cell line and the progeny that underwent EMT and shows that specific histone methylations are extensively involved in gene expression reprogramming during EMT and subsequent accumulation of malignant features. The observation that transcription activity of bivalently marked genes depends on the relative labeling intensity of individual marks provides a new view of quantitative regulation of epigenetic modification.</p
- …