Glossopexy for upper airway obstruction in a patient with Pierre Robin Sequence

Introduction: Pierre Robin sequence (PRS) is a congenital malformation. One of its typical sign is micrognathia, which often causes retroposition of the tongue with possible significant airway obstruction and feeding difficulties. The symptoms usually disappear around one year of the age, when the lower jaw, the oropharynx, and tongue musculature are more developed. If conservative treatment is not sufficient, a surgical procedure such as a tongue-lip adhesion can be performed. Material/Methods: The case report of premature twin baby with PRS is presented. The baby was born at 27th gestation week by Caesarean operation with typical signs of PRS included micrognatia. Immediate postnatal adaptation was good. However, at the age of four month repeated episodes of desaturation occurred. Retroposition of the tongue and very narrow retrolingual space was diagnosed using flexible endoscopy. Repeated episodes of desaturation happened again, so that labioglossopexy was performed. Dehiscence of the suture with repeated episodes of desaturation occurred 11th day after surgery. So that a new tongue-lip adhesion was performed. Further course was uneventful. Three month after surgrery is child doing well, without any episodes of desaturation.Results: Labioglossopexy (tongue-lip adhesion) is an effective procedure of treatment the respiratory obstruction associated with retroposition of the tongue typical for PRS. Surgical technique and its advantages and possible complications are discussed.Conclusions: Labioglossopexy in patients with PRS should be balanced against the other operations, namely tracheostomy and mandibular distraction. Labioglossopexy is less invasive method then tracheostomy, simplifies nursing care, shortens hospital stay and makes homecare less demanding.Der Erstautor gibt keinen Interessenkonflikt an

High Throughput Sequencing of siRNAs and virus diagnostic: does sequence analysis strategies really matter? Results of an international proficiency testing

High Throughput Sequencing of siRNAs and virus diagnostic: does sequence analysis strategies really matter? Results of an international proficiency testing. 16. Rencontres de Virologie Végétale (RVV 2017

Virus detection by high-throughput sequencing of small RNAs: large scale performance testing of sequence analysis strategies.

Recent developments in high-throughput sequencing (HTS), also called next-generation sequencing (NGS), technologies and bioinformatics have drastically changed research on viral pathogens and spurred growing interest in the field of virus diagnostics. However, the reliability of HTS-based virus detection protocols must be evaluated before adopting them for diagnostics. Many different bioinformatics algorithms aimed at detecting viruses in HTS data have been reported, but little attention has been paid so far to their sensitivity and reliability for diagnostic purposes. We therefore compared the ability of 21 plant virology laboratories, each employing a different bioinformatics pipeline, to detect 12 plant viruses through a double-blind large scale performance test ten datasets of 21-24 nt small (s)RNA sequences from three different infected plants. The sensitivity of virus detection ranged between 35 and 100% among participants, with a marked negative effect when sequence depth decreased. The false positive detection rate was very low and mainly related to the identification of host genome-integrated viral sequences or misinterpretation of the results. Reproducibility was high (91.6%). This work revealed the key influence of bioinformatics strategies for the sensitive detection of viruses in HTS sRNA datasets and, more specifically (i) the difficulty to detect viral agents when they are novel and/or their sRNA abundance is low, (ii) the influence of key parameters at both assembly and annotation steps, (iii) the importance of completeness of reference sequence databases and (iv) the significant level of scientific expertise needed when interpreting pipelines results. Overall, this work underlines key parameters and proposes recommendations for reliable sRNA-based detection of known and unknown viruses.Diversification for Tobacco Growing Farms by the alternative crop Stevia rebaudiana Berton