Discrimination and Quantification of Glomerular Receptor Subtypes for Atrial Natriuretic Factor (Anf)

Binding sites for atrial natriuretic factor (ANF) were determined on isolated rat glomeruli as well as on glomerular membranes. To define optimal conditions, binding of ANF was investigated varying incubation time, temperature and protein concentration. Binding conditions were found to be best at 4°C for 5 hours with 15 μg of glomerular protein. Saturation and affinity cross-linking experiments confirmed the presence of two distinct receptor subtypes - the B-receptor (130 kDa) and the C-receptor (65 kDa). Quantitative differentiation of both ANF binding sites was achieved by competitive displacement with two different unlabeled ANF ligands: a) rANF(99-126) (homologous displacement), b) des(18-22)rANF(4-23)NH2(heterologous displacement). Intact glomeruli and glomerular membranes did not differ significantly in receptor density for the B-receptor (71 ± 37 vs. 94 ± 53 fmol/mg protein) or the C-receptor (976 ± 282 vs. 966 ± 167 fmol/mg protein) or in affinity constants for the B-receptor (43 ± 36 vs. 52 ± 44 pM) or the C-receptor (876 ± 377 vs. 307 ± 36 pM). Glomerular membranes compared to glomeruli showed less nonspecific binding and less intra-assay variation of measuring points done in triplicates. This method of selective displacement should allow to study the influence of various physiological and pathophysiological conditions on the binding properties of B-and C-receptors for ANF

Altered density of glomerular binding sites for atrial natriuretic factor in bile duct-ligated rats with ascites

The renal response to atrial natriuretic factor is blunted in cirrhosis with ascites. This might be due to alterations of renal receptors for atrial natriuretic factor. Therefore density and affinity of glomerular atrial natriuretic factor binding sites of bile duct-ligated rats with ascites (n = 10) and of sham-operated controls (n = 10) were determined. Glomerular atrial natriuretic factor binding sites were identified to be of the B-(biologically active) and C-(clearance) receptor type. Discrimination and quantitative determination of B and C receptors for atrial natriuretic factor were achieved by displacement experiments with atrial natriuretic factor(99-126) or des(18-22)atrial natriuretic factor(4-23), an analogue binding to C receptors only. Density of total glomerular atrial natriuretic factor binding sites was significantly increased in bile duct-ligated rats (3,518 ± 864 vs. 1,648 ± 358 fmol/mg protein; p < 0.05). This was due to a significant increase of C-receptor density (3,460 ± 866 vs. 1,486 ± 363 fmol/mg protein; p < 0.05), whereas density of B receptors was not significantly different in bile duct-ligated rats (58 ± 11 vs. 162 ± 63 fmol/mg protein). Affinity of atrial natriuretic factor to its glomerular binding sites did not differ significantly between both groups. These data suggest that an altered glomerular atrial natriuretic factor receptor density could be involved in the renal resistance to atrial natriuretic factor in cirrhosis with ascites

Optimizing precision agricultural operations by standardized cloud-based functions

Aim of study: An approach to integrate knowledge into the IT-infrastructure of precision agriculture (PA) is presented. The creation of operation relevant information is analyzed and explored to be processed by standardized web services and thereby to integrate external knowledge into PA. The target is to make knowledge integrable into any software solution. Area of study: The data sampling took place at the Heidfeld Hof Research Station in Stuttgart, Germany. Material and methods: This study follows the information science’s idea to separate the process from data sampling into the final actuation through four steps: data, information, knowledge, and wisdom. The process from the data acquisition, over a professional data treatment to the actual application is analyzed by methods modelled in the Unified Modelling Language (UML) for two use-cases. It was further applied for a low altitude sensor in a PA operation; a data sampling by UAV represents the starting point. Main results: For the implemented solution, the Web Processing Service (WPS) of the Open Geospatial Consortium (OGC) is proposed. This approach reflects the idea of a function as a service (FaaS), in order to develop a demand-driven and extensible solution for irregularly used functionalities. PA benefits, as on-farm processes are season oriented and a FaaS reflects the farm’s variable demands over time by origin and extends the concept to offer external know-how for the integration into specific processes. Research highlights: The standardized implementation of knowledge into PA software products helps to generate additional benefits for PA

Position-Independent Code Reuse:On the Effectiveness of ASLR in the Absence of Information Disclosure

Address-space layout randomization is a wellestablished defense against code-reuse attacks. However, it can be completely bypassed by just-in-time code-reuse attacks that rely on information disclosure of code addresses via memory or side-channel exposure. To address this fundamental weakness, much recent research has focused on detecting and mitigating information disclosure. The assumption being that if we perfect such techniques, we will not only maintain layout secrecy but also stop code reuse. In this paper, we demonstrate that an advanced attacker can mount practical code-reuse attacks even in the complete absence of information disclosure. To this end, we present Position-Independent Code-Reuse Attacks, a new class of codereuse attacks relying on the relative rather than absolute location of code gadgets in memory. By means of memory massaging, the attacker first makes the victim program generate a rudimentary ROP payload (for instance, containing code pointers that target instructions 'close' to relevant gadgets). Afterwards, the addresses in this payload are patched with small offsets via relative memory writes. To establish the practicality of such attacks, we present multiple Position-Independent ROP exploits against real-world software. After showing that we can bypass ASLR in current systems without requiring information disclosures, we evaluate the impact of our technique on other defenses, such as fine-grained ASLR, multi-variant execution, execute-only memory and re-randomization. We conclude by discussing potential mitigations