Generating Rental Data for Car Sharing Relocation Simulations on the Example of Station-Based One-Way Car Sharing

Developing sophisticated car sharing simulations is a major task to improve car sharing as a sustainable means of transportation, because new \ algorithms for enhancing car sharing efficiency are formulated using them. \ \ Simulations rely on input data, which is often gathered in car sharing systems or artificially generated. Real-world data is often incomplete and biased while artificial data is mostly generated based on initial assumptions. Therefore, developing new ways for generating testing data is an important task for future research. \ \ In this paper, we propose a new approach for generating car sharing data for relocation simulations by utilizing machine learning. Based on real-world data, we could show that a combined methods approach consisting of a Gaussian Mixture Model and two classification trees can generate appropriate artificial testing data

Gesundheitswirkung städtischer Grünräume: eine empirische Analyse

Neben der positiven Wirkung für die Luftqualität, den Klimaschutz und die Biodiversität, wird seit Längerem auch auf die Potenziale des städtischen Grüns für die menschliche Gesundheit verwiesen. Demgegenüber steht die sehr lückenhafte empirische Basis bezüglich des Zusammenhangs zwischen städtischen Grünflächen und der Gesundheit in Deutschland. Im Mittelpunkt dieses Beitrags steht eine evidenzbasierte Untersuchung des Zusammenhangs von städtischen Freiflächen und dem menschlichen Wohlbefinden. Dazu wurden ­Gesundheitsdaten des Sozioökonomischen Panels (SOEP) für die Jahre 2000 – 2012 mit georeferenzierten Landnutzungsdaten des European Urban Atlas (EUA) verschnitten. Die Ergebnisse belegen einen positiven Zusammenhang zwischen einer verbesserten Verfügbarkeit von städtischem Grün und der Lebenszufriedenheit sowie der mentalen und physischen Gesundheit. Demgegenüber übt die Anwesenheit von städtischen Brachflächen einen negativen Einfluss auf das Wohlbefinden der befragten Haushalte aus. Obwohl die konkreten Wirk­mechanismen der positiven Effekte von Grünflächen auf die Gesundheit nicht Gegenstand der Untersuchung waren, kann vermutet werden, dass die Grünflächen als Bewegungs

The greener, the happier?: the effect of urban land use on residential well-being

We investigate the effect of urban land use on residential well-being in major German cities, using panel data from the German Socio-Economic Panel and cross-section data from the European Urban Atlas. We reduce concerns about endogeneity by employing fixed-effects (within) estimators, with individual and city of residence fixed effects, while controlling for a rich set of observables. The results show that access to green urban areas, such as gardens and parks, is positively associated with, whereas access to abandoned areas, such as waste or leftover land, is negatively associated with life satisfaction. The effects are strongest for residents who are older, accounting for up to a third of the size of the effect of being unemployed on life satisfaction. We calculate the marginal willingness-to-pay of residents in order to have access to green urban and abandoned areas in their surroundings, as well as the life-satisfaction maximising amounts of them. Finally, we provide a policy case study, while discussing limitations and avenues for future research

90K, an interferon-stimulated gene product, reduces the infectivity of HIV-1

BACKGROUND: In response to viral infections, interferons induce the transcription of several hundred genes in mammalian cells. Specific antiviral functions, however, have only been attributed to a few of them. 90K/LGALS3BP has been reported to be an interferon-stimulated gene that is upregulated in individuals with cancer or HIV-1 infection. RESULTS: Here, we show that 90K expression dose-dependently decreased the particle infectivity of HIV-1 progeny. The lower infectivity of released particles correlated with reduced virion incorporation of mature envelope glycoproteins gp120 and gp41. Further, proteolytic processing of the gp160 precursor and surface expression of gp120 in the producer cell were impaired in the presence of 90K expression. In contrast, expression of Gag, Nef and Vpu, and virus release were not grossly affected by 90K expression. 90K-imposed restriction occurred in the absence of direct interaction of 90K with HIV-1 Env or entrapment of Env in the ER. The cell-associated, but not the secreted species of 90K, mediated the antiviral effect. A truncated version of human 90K, solely consisting of the two intermediate domains, displayed a similar antiviral activity as the full-length wildtype 90K, indicating that the N-terminal SRCR-like domain and the C-terminal domain are dispensable for 90K’s antiviral activity. The murine homolog of 90K, CypCAP (Cyclophilin C-associated protein), neither modulated particle infectivity of HIV-1 nor lowered the virion incorporation of mature gp120, suggesting a species-specific mode of action. 90K was expressed at basal levels in TZM-bl cells and in primary macrophages, and at low levels in CD4(+) T-cells and PBMCs. 90K’s susceptibility to IFN-mediated stimulation of expression was cell type-specific. siRNA-mediated knockdown of 90K in TZM-bl cells and primary macrophages enhanced the incorporation of Env glycoproteins into progeny virions, boosted the particle infectivity of released HIV-1, and accelerated HIV-1 spread. Conversely, treatment of HIV-1 infected macrophages with IFN-α induced 90K expression and lowered the particle infectivity of HIV-1. CONCLUSIONS: Thus, 90K constitutes a novel antiviral factor that reduces the particle infectivity of HIV-1, involving interference with the maturation and incorporation of HIV-1 Env molecules into virions

Urban green is more than the absence of city: Structural and functional neural basis of urbanicity and green space in the neighbourhood of older adults

The relationship between urbanization, the brain, and human mental health is subject to intensive debate in the current scientific literature. Particularly, since mood and anxiety disorders as well as schizophrenia are known to be more frequent in urban compared to rural regions. Here, we investigated the association between cerebral signatures, mental health and land use indicators (Urban Fabric and Urban Green) within a 1 km radius around the home address of 207 well-characterized older adults. We observed a negative association between Urban Fabric coverage and a positive association between Urban Green coverage and grey matter volume in perigenual/subgenual anterior cingulate cortex (p/sACC). Although p/sACC has repeatedly been associated with depressive symptoms, neither brain structure nor land use categories were related to measures of mental health. However, resting-state measure in p/sACC showed a negative association with Urban Fabric in our healthy sample, reminiscent of previous reports on major depression where p/sACC is often found to be reduced in activation. Interestingly, hierarchical regression analyses showed that Urban Green accounted for additional variance in brain structure beyond Urban Fabric. We take this finding as an exploratory result that hints at potentially salutogenic elements of green spaces (e.g. terpenes, nature sounds) that go beyond the absence of the detrimental elements of urban contexts (e.g. traffic noise, air pollution), which may inform the future search of environmental factors affecting mental health and disease

Genome-wide analysis of PAPS1-dependent polyadenylation identifies novel roles for functionally specialized poly(A) polymerases in Arabidopsis thaliana

The poly(A) tail at 3’ ends of eukaryotic mRNAs promotes their nuclear export, stability and translational efficiency, and changes in its length can strongly impact gene expression. The Arabidopsis thaliana genome encodes three canonical nuclear poly(A) polymerases,PAPS1, PAPS2 and PAPS4. As shown by their different mutant phenotypes, these three isoforms are functionally specialized, with PAPS1 modifying organ growth and suppressing a constitutive immune response. However, the molecular basis of this specialization is largely unknown. Here, we have estimated poly(A)-tail lengths on a transcriptome-wide scale in wild-type and paps1 mutants. This identified categories of genes as particularly strongly affected in paps1 mutants, including genes encoding ribosomal proteins, cell-division factors and major carbohydrate-metabolic proteins. We experimentally verified two novel functions of PAPS1 in ribosome biogenesis and redox homoeostasis that were predicted based on the analysis of poly(A)-tail length changes in paps1 mutants. When overlaying the PAPS1-dependent effects observed here with coexpression analysis based on independent microarray data, the two clusters of transcripts that are most closely coexpressed with PAPS1 show the strongest change in poly(A)-tail length and transcript abundance in paps1 mutants in our analysis. This suggests that their coexpression reflects at least partly the preferential polyadenylation of these transcripts by PAPS1 versus the other two poly(A)-polymerase isoforms. Thus, transcriptome-wide analysis of poly(A)-tail lengths identifies novel biological functions and likely target transcripts for polyadenylation by PAPS1. Data integration with large-scale co-expression data suggests that changes in the relative activities of the isoforms are used as an endogenous mechanism to co-ordinately modulate plant gene expression

Location, location, location: the role of objective neighborhood characteristics for perceptions of control

Introduction: Control beliefs can protect against age-related declines in functioning. It is unclear whether neighborhood characteristics shape how much control people perceive over their life. This article studies associations of neighborhood characteristics with control beliefs of residents of a diverse metropolitan area (Berlin, Germany). Methods: We combine self-report data about perceptions of control obtained from participants in the Berlin Aging Study II (N = 507, 60-87 years, 51% women) with multisource geo-referenced indicators of neighborhood characteristics using linear regression models. Results: Findings indicate that objective neighborhood characteristics (i.e., unemployment rate) are indeed tied to perceptions of control, in particular, how much control participants feel others have over their lives. Including neighborhood characteristics in part doubled the amount of explained variance compared with a reference model covarying for demographic characteristics only (from R2 = 0.017 to R2 = 0.030 for internal control beliefs; R2 = 0.056 to R2 = 0.102 for external control beliefs in chance; R2 = 0.006 to R2 = 0.030 for external control beliefs in powerful others). Discussion/Conclusion: Findings highlight the importance of access to neighborhood resources for control beliefs across old age and can inform interventions to build up neighborhood characteristics which might be especially helpful in residential areas with high unemployment