10 research outputs found
Functional annotation of human long noncoding RNAs via molecular phenotyping
Long noncoding RNAs (lncRNAs) constitute the majority of transcripts in the mammalian genomes, and yet, their functions remain largely unknown. As part of the FANTOM6 project, we systematically knocked down the expression of 285 lncRNAs in human dermal fibroblasts and quantified cellular growth, morphological changes, and transcriptomic responses using Capped Analysis of Gene Expression (CAGE). Antisense oligonucleotides targeting the same lncRNAs exhibited global concordance, and the molecular phenotype, measured by CAGE, recapitulated the observed cellular phenotypes while providing additional insights on the affected genes and pathways. Here, we disseminate the largest-todate lncRNA knockdown data set with molecular phenotyping (over 1000 CAGE deep-sequencing libraries) for further exploration and highlight functional roles for ZNF213-AS1 and lnc-KHDC3L-2.Peer reviewe
New Concepts Concerning Prostate Cancer Screening
Prostate Cancer (CaP) is rapidly becoming a worldwide health issue. While CaP mortality has decreased in recent years, coincident with the widespread use of Prostate-Specific Antigen (PSA) screening, it remains the most common solid tumor in men and is the second leading cause of cancer death in the United States. The frequency of CaP is growing not only in western cultures, but also its incidence is dramatically increasing in eastern nations. Recently, examination of data from long-term trials and follow up has cast a shadow on the effectiveness of employing PSA as a primary screening tool for CaP. In this review, we not only summarize opinions from this examination and synthesize recommendations from several groups that suggest strategies for utilizing PSA as a tool, but also call for research into biomarkers for CaP diagnosis and disease progression. We also describe our recent work that identified a smooth muscle contractile protein in prostate epithelia, namely smooth muscle gamma actin, and indicate the potential for this molecule as a new unique footprint and as a CaP marker. © 2014 by the Society for Experimental Biology and Medicine
IGARSS 2019 in Yokohama, Japan: Events and New Directions
The 39th IEEE International Geoscience and Remote Sensing Symposium (IGARSS 2019), held 28 July–2 August 2019 at the Pacifico Yokohama Convention Center, Japan, welcomed more than 2,500 participants from 56 countries and areas. The local organizing com-mittee (LOC) received positive feedback from many participants, who described the venue and conference as being very convenient, well organized, and most enjoyable. The general chair attributes these positive evaluations to strong LOC teamwork. The goal of pro-viding the “best experience for all participants,” which IEEE Geoscience and Remote Sensing Society (GRSS) President Paolo Gamba and I confirmed at the GRSS administrative committee meetings, was shared by all of the LOC members.One of the highlights of the symposium was the opening ceremony, which was held in a solemn but warm atmosphere on 29 July with Their Majesties the Emperor and Empress of Japan in attendance. In his address, His Majesty the Emperor referred to the importance of detailed and accurate data acqui-sition and analysis, which greatly encouraged the participants working in the geoscience and remote sensing field.In this column, the LOC cochairs, who supervised the harmonious teamwork, revisit the events and re-view the activities, statistics, and logistics, which will be helpful for future IGARSS events. Following each of the following sections, the chairs’ names are provided so that readers can contact them. The “Conference Re-port” column “IGARSS in Yokohama, Japan: Impres-sions From the First Days” in the September 2010 issue of IEEE Geoscience and Remote Sensing Magazine [1] i s also informative
Food intake and food consumption patterns of hospital workers in the dominican republic
Coronary heart disease (CHD) is the leading cause of death according to official mortality statistics in the Dominican Republic. The purpose of this study was to assess food intake and food consumption patterns of Dominicans and to discuss CHD risks related to nutrient intake. The subjects were 22 men and 81 women voluntarily recruited among 230 hospital workers in Santo Domingo. The mean nutrient intake and food consumption were estimated from a single 24-hour recall method administered by trained persons who elicited each subject’s previous day’s intake from midnight to midnight. The mean energy intake was 2030 kcal. The mean intake of total protein, total fat and carbohydrates were 66.3g, 89.1 g and 248g, respectively. Percentage of energy from total fat was 39.5%, which was higher than WHO recommendations. But, the percentage of animal fat per total fat was 46.8%. Especially, the value in the low income group was 31.7%. Moreover, we analyzed the food consumption structure in order to understand the Dominican dietary pattern, and three factors were found to explain the characteristics on the basis of factor analysis