The diary of Robert John Mullins (1833-1913)

Summary of Content: This volume offers its readers extracts from the diary and the correspondence of Robert John Mullins, Anglican missionary, priest and in due time principal of what was called the Kafir Institution in Grahamstown and as such a pioneer in the field of western education to black South Africans. After his marriage to Jennie Roe he was assisted in all that he undertook by a loyal and devoted wife. The editors have made use of the earlier diaries and manuscripts because of the light they shed on the conditions in the eastern Cape frontier in the crucial years before and after the cattle-killing. While Mullins' own observations are those of a young missionary his remarks and the record of his experiences are themselves to be viewed in the light of the recent scholarship in regard to the cattle-killing which reveals the multisidedness of an appalling social tragedy. What Mullins wrote records, for better or for worse, episodes which show how South Africans of different origins have in the past regarded one another. Mullins was a committed Christian eager to offer to all whom he could reach the salvation of Christ in which he so fervently believed.He was often intolerant of Xhosa customs he could not understand as well as often perplexed and discouraged. But the overwhelming impact of this record is what it took to be a missionary: courage, commitment, humour, resilience, a capacity to endure physical hardship and enjoy physical activity. All these were qualities which Robert John Mullins possessed in abundance. As his diary shows they were the qualities the frontier demanded. Working over a long period of time Nancy Charton and Brenda Nicholls (who became a coeditor in 1991) have transcribed the diary entries and the correspondence working with the help of the sites of some of the missionary activity. The help of members of the Mullins family (notably Judy Sturrock and her husband) is gratefully acknowledged. Earlier generous contributions made it possible to obtain a typescript from which the editors could work. Every effort has been made to identify the individuals (Xhosa, Tembu and white) and events (both in South Africa and abroad) which are alluded to in the diary. This was a time consuming task and the editors are grateful to all who helped them with it. The book consists of an introduction and eleven chapters. The introduction describes the material and the methods of the editors, discusses the religious and social conditions of the time, addresses the problems of interpartition presented by the cattle-killings, turns attention to the dilemmas of missionaries and then focusses the attention of readers on the diarist Robert John Mullins (with Jennie as later stand-in) as well as the value of the diary. There is much to interest the educationist, the theologian, the economist, and the historian. Chapter 1 deals with Mullins' voyage to the Cape in 1854 and the extracts record the experiences of an exuberant as well as a dedicated lad of sixteen. Chapter 2 deals with the arrival at the Cape in 1854. Chapter 3 gives us a picture of Mullins' experience of Graham's Town in 1854. Chapter 4 deals with Mullins' experience at St Luke's Mission. Chapter 5 covers the period of 1856-57 when Mullins was at Balotwa and the cattle-killing frenzy developed. Chapter 6 describes the hunger and turmoil which followed in the wake of the cattle-killing. Chapter 7 describes the founding of a new station, St Peter's Gwytyu. Chapter 8 is concerned with life at St Peter's in the early months of 1858. Chapter 9 covers the last months at St Peter's and his journey to the Bashee. Chapters 10 and 11 cover the early years in the married life of Robert John Mullins and his wife Jennie. The Mullins' delight in family life is strongly evident in their joy in each other's company and their anxious joy over their first-born baby. Chapter 11 gives the reader the texts of letters written by Robert John and his wife Jennie in the years which marked the end of Mullins' career as a frontier missionary, and the opening to him of the opportunity to become Principal of the Kafir Institution. A map carefully prepared by Mr Oakley West of the Geography Department, with the assistance of Deby Brody, enables the reader to trace the frontier journeys of Robert John Mullins and his wife Jennie who were among the pioneers of frontier societies who did their best to make available to others what they believed to be the eternal benefits of the Christian faith, and the accompanying mores of the 'western' culture of their day

Linewidths in bound state resonances for helium scattering from Si(111)-(1x1)H

Helium-3 spin-echo measurements of resonant scattering from the Si(111)–(1 × 1)H surface, in the energy range 4–14 meV, are presented. The measurements have high energy resolution yet they reveal bound state resonance features with uniformly broad linewidths. We show that exact quantum mechanical calculations of the elastic scattering, using the existing potential for the helium/Si(111)–(1 × 1)H interaction, cannot reproduce the linewidths seen in the experiment. Further calculations rule out inelastic and other mechanisms that might give rise to losses from the elastic scattering channels. We show that corrugation in the attractive part of the atom–surface potential is the most likely origin of the experimental lineshapes

Substrate protein folds while it is bound to the ATP-independent chaperone Spy

Chaperones assist the folding of many proteins in the cell. While the most well studied chaperones use cycles of ATP binding and hydrolysis to assist protein folding, a number of chaperones have been identified that promote protein folding in the absence of highenergy cofactors. Precisely how ATP-independent chaperones accomplish this feat is unclear. Here we have characterized the kinetic mechanism of substrate folding by the small, ATP-independent chaperone, Spy. Spy rapidly associates with its substrate, Immunity protein 7 (Im7), eliminating its potential for aggregation. Remarkably, Spy then allows Im7 to fully fold into its native state while remaining bound to the surface of the chaperone. These results establish a potentially widespread mechanism whereby ATP-independent chaperones can assist in protein refolding. They also provide compelling evidence that substrate proteins can fold while continuously bound to a chaperone

The Vision of Unity

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Cable & Wireless – past, present and future: the telecom giant awakens

Presents a current overview of the UK telecommunications group Cable & Wireless. Describes its existing global business, with subsidiaries in over 50 countries and links with many major companies and overseas governments. Discusses the development of communications, from copper cables transmitting Morse code to modern fibre optic strands using laser light, reviewing the introduction of satellite and digital technology, computers and the Internet. Debates the convergence and possible future combination of these technologies in a multimedia communications revolution. Examines the precedents set in the early 20th century with mergers of cable and radio companies, and considers the possibility of similar mergers in the future between cable, satellite, computer and media businesses. Describes C&W’s involvement with Microsoft and BSkyB in areas such as: Web TV; set-top boxes; video-on-demand and digitization

Conformational dynamics is more important than helical propensity for the folding of the all alpha-helical protein Im7

Im7 folds via an on-pathway intermediate that contains three of the four native α-helices. The missing helix, helix III, is the shortest and its failure to be formed until late in the pathway is related to frustration in the structure. Im7H3M3, a 94-residue variant of the 87-residue Im7 in which helix III is the longest of the four native helices, also folds via an intermediate. To investigate the structural basis for this we calculated the frustration in the structure of Im7H3M3 and used NMR to investigate its dynamics. We found that the native state of Im7H3M3 is highly frustrated and in equilibrium with an intermediate state that lacks helix III, similar to Im7. Model-free analysis identified residues with chemical exchange contributions to their relaxation that aligned with the residues predicted to have highly frustrated interactions, also like Im7. Finally, we determined properties of urea-denatured Im7H3M3 and identified four clusters of interacting residues that corresponded to the α-helices of the native protein. In Im7 the cluster sizes were related to the lengths of the α-helices with cluster III being the smallest but in Im7H3M3 cluster III was also the smallest, despite this region forming the longest helix in the native state. These results suggest that the conformational properties of the urea-denatured states promote formation of a three-helix intermediate in which the residues that form helix III remain non-helical. Thus it appears that features of the native structure are formed early in folding linked to collapse of the unfolded state

Direct Comparison of Label-Free Biosensor Binding Kinetics Obtained on the Biacore 8K and the Carterra LSA

Funding pressure on the pharmaceutical industry to deliver new medicines to the market under aggressive timelines has led to a demand for analytical tools with higher detection sensitivity, increased throughput, and automation to speed up research and discovery efforts and converge upon clinically fit leads faster. In the quest for therapeutic antibodies, the early adoption of interaction analysis platforms utilizing surface plasmon resonance (SPR) detection provides insightful molecular-level information about the binding properties of antibody libraries that are key to understanding an antibody’s mechanism of action and can guide the library-to-leads triage. Here, we sought to compare the binding kinetics obtained on two state-of-the-art high-throughput SPR platforms in an independent study conducted by unrelated groups located on different continents. We show that when experiments were performed by skilled users adhering to SPR best practices and allowed freedom in their assay design, the two platforms yielded near-identical results, establishing them both as reliable tools in accelerating the characterization of antibody libraries in providing critical information needed to advance leads to the clinic

A novel Transcript is Up‐Regulated by Fasting in the Hypothalamus and Enhances Insulin Signalling

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/96718/1/jne2378.pd