Manual of Quaternions

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Effects of atmospheric CO2 on photosynthetic characteristics of soybean leaves

Soybean (Glycine max. cv. McCall) plants were grown at 500, 1000, and 2000 umol mol (exp -1) CO2 for 35 days with a photosynthetic photon flux of 300 umol m (exp -2) s (-1). Individual leaves were exposed to step changes of photosynthetic photon flux to study CO2 assimilation rates (CAR), i.e., leaf net photosynthesis. In general, CAR increased when CO2 increased from 500 to 1000 umol mol (exp -1), but not from 1000 to 2000 umol mol (exp -1). Regardless of the CO2 level, all leaves showed similar CAR at similar CO2 and PPF. This observation contrasts with reports that plants tend to become 'lazy' at elevated CO2 levels over time. Although leaf stomatal conductance (to water vapor) showed diurnal rhythms entrained to the photoperiod, leaf CAR did not show these rhythms and remained constant across the light period, indicating that stomatal conductance had little effect on CAR. Such measurements suggest that short-term changes in CO2 exchange dynamics for a controlled ecological life support system can be closely predicted for an actively growing soybean crop

Calcium binding activity of the epidermal growth factor-like domains of the apicomplexan microneme protein EtMIC4

Microneme proteins are secreted from apicomplexan parasites during invasion of host cells and they play crucial roles in parasite-host cell adhesion. EtMIC4 is a 240 kDa transmembrane protein from Eimeria tenella that contains 31 tandemly arranged epidermal growth factor (EGF), like repeats within its extracellular domain. The majority of these repeats have calcium binding (cb) consensus sequences. Little is known about cbEGFs in apicomplexan parasites but their presence in microneme proteins suggests that they may contribute to parasite-host interactions. To investigate the potential role of cbEGFs we have expressed and correctly refolded a cbEGF triplet from EtMIC4 (cbEGF7-9) and demonstrated that this triplet binds calcium. Circular dichroism spectroscopic analysis of cbEGF7-9 demonstrates that the molecule undergoes a gradual change in conformation with increasing levels of calcium. In the presence of calcium, the triplet becomes resistant to proteolytic degradation by a variety of proteases, a characteristic feature of cbEGF repeats from higher eukaryotic proteins, such as fibrillin, suggesting that calcium binding induces the formation of a rigid conformation. Moreover, mass spectrometric mapping of the cleavage sites that are protected by calcium shows that these sites are located both close to and distant from the calcium binding sites, indicating that protection is not due to steric hindrance by calcium ions, but rather due to the overall conformation adopted by the triplet in the presence of calcium. Thus, the tandemly-arranged cbEGF repeats within EtMIC4 provide a mechanism whereby, in the calcium-rich extracellular environment, the molecule could adopt a protease-resistant, rigid structure that could favour its interaction with host cell ligands

Hyperkalaemic complete heart block : a report of 2 unique cases and a review of the literature

The original publication is available at http://www.samj.org.zaTwo White male patients with temporary complete heart block (CHB) secondary to hyperkalaemia are presented. One, a 40-year-old man, developed CHB with ensuing shock within the first 24 hours of repeat aortic valve replacement for a paraprosthetic leak caused by previous endocarditis. This patient experienced iatrogenic hyperkalaemia. The second was an 81-year-old man who had chronic renal failure and presented with Stokes-Adams attacks. This patient was initially thought to have degenerative CHB and nearly underwent inadvertent permanent pacemaker insertion. Both patients were initially treated with emergency temporary cardiac pacing with subsequent successful management. Temporary CHB secondary to hyperkalaemia, from whatever cause, has very rarely been documented in the literature. A review of this potentially lethal complication is undertaken and the significance of unifascicular and bifascicular conduction block as a consequenc of hyperkalaemia is discussed.Publishers' versio

Engineering verification of the biomass production chamber

The requirements for life support systems, both biological and physical-chemical, for long-term human attended space missions are under serious study throughout NASA. The KSC 'breadboard' project has focused on biomass production using higher plants for atmospheric regeneration and food production in a special biomass production chamber. This chamber is designed to provide information on food crop growth rate, contaminants in the chamber that alter plant growth requirements for atmospheric regeneration, carbon dioxide consumption, oxygen production, and water utilization. The shape and size, mass, and energy requirements in relation to the overall integrity of the biomass production chamber are under constant study

Attaining subclassical metrology in lossy systems with entangled coherent states

Quantum mechanics allows entanglement enhanced measurements to be performed, but loss remains an obstacle in constructing realistic quantum metrology schemes. However, recent work has revealed that entangled coherent states (ECSs) have the potential to perform robust subclassical measurements [J. Joo et al., Phys. Rev. Lett. 107, 083601 (2011)]. Up to now no read-out scheme has been devised that exploits this robust nature of ECSs, but we present here an experimentally accessible method of achieving precision close to the theoretical bound, even with loss.We show substantial improvements over unentangled classical states and highly entangled NOON states for a wide range of loss values, elevating quantum metrology to a realizable technology in the near future

Metal-insulator transition and electroresistance in lanthanum/calcium manganites La_<1-x>Ca_<x>MnO_<3> (x = 0-0.5) from voltage-current-temperature surfaces

Of the perovskites, ABX_, a subset of special interest is the family in which the A site is occupied by a lanthanide ion, the B site by a rare earth and X is oxygen, as such materials often exhibit a large change in electrical resistance in a magnetic field, a phenomenon known as "colossal" magnetoresistance (MR). Two additional phenomena in this family have also drawn attention: the metal-insulator transition (MIT) and electroresistance (ER). The MIT is revealed by measuring resistance as a function of temperature, and observing a change in the sign of the gradient. ER - the dependence of the resistance on applied current - is revealed by measuring resistance as a function of applied current. Up until now, the phenomena of MIT and ER have been treated separately. Here we report simultaneous observation of the MIT and ER in the lanthanum/calcium manganites. We accomplish this by measuring voltage-current curves over a wide temperature range (10-300 K) allowing us to build up an experimental voltage surface over current-temperature axes. These data directly lead to resistance surfaces. This approach provides additional insight into the phenomena of electrical transport in the lanthanum/calcium manganites, in particular the close connection of the maximum ER to the occurrence of the MIT in those cases of a paramagnetic insulator (PMI) to ferromagnetic metal (FMM) transition.Comment: 15 pages, 12 figure

Where surface physics and fluid dynamics meet: rupture of an amphiphile layer by fluid flow

We investigate the fluctuating pattern created by a jet of fluid impingent upon an amphiphile-covered surface. This microscopically thin layer is initially covered with 50 $\mu$m floating particles so that the layer can be visualized. A vertical jet of water located below the surface and directed upward drives a hole in this layer. The hole is particle-free and is surrounded by the particle-laden amphiphile region. The jet ruptures the amphiphile layer creating a particle-free region that is surrounded by the particle-covered surface. The aim of the experiment is to understand the (fluctuating) shape of the ramified interface between the particle-laden and particle-free regions.Comment: published in Journal of Chemical Physic

A summary of porous tube plant nutrient delivery system investigations from 1985 to 1991

The Controlled Ecological Life Support System (CELSS) Program is a research effort to evaluate biological processes at a one person scale to provide air, water, and food for humans in closed environments for space habitation. This program focuses currently on the use of conventional crop plants and the use of hydroponic systems to grow them. Because conventional hydroponic systems are dependent on gravity to conduct solution flow, they cannot be used in the microgravity of space. Thus, there is a need for a system that will deliver water and nutrients to plant roots under microgravity conditions. The Plant Space Biology Program is interested in investigating the effect that the space environment has on the growth and development of plants. Thus, there is also a need to have a standard nutrient delivery method for growing plants in space for research into plant responses to microgravity. The Porous Tube Plant Nutrient Delivery System (PTPNDS) utilizes a hydrophilic, microporous material to control water and nutrient delivery to plant roots. It has been designed and analyzed to support plant growth independent of gravity and plans are progressing to test it in microgravity. It has been used successfully to grow food crops to maturity in an earth-bound laboratory. This document includes a bibliography and summary reports from the growth trials performed utilizing the PTPNDS