The effect of cultivation conditions on the growing processes of grape plants

One of alternative ways to maintain valuable genetic material is to develop optimal conditions for cultivation in an in vitro system. The goal was to evaluate the condition of plants based on changes in the shoot length indicator in order to explore the maintaining mode of the collection. We took the experimental samples from the “Vegetating collection of plants in vitro of promising varieties and clones of grapes”: 13 local Crimean varieties and 9 varieties of the Institute Magarach selection. Plant containing culture bottles were in the cold storage without internal lighting at 10-12°C for 6 months. The cultivation findings demonstrated that grape varieties differed in viability and intensity of morphogenesis. Moreover, after storage, they had a high regenerating ability of the buds

Estimation of Damage Development and the TIME of Failure of Cutting Inserts Made of Hard Alloys and Superhard Composites by Chemography Methods

The results of theoretical and experimental studies aimed at identifying hidden defects in the structure of hard alloys and superhard composites used in the manufacture of cutting tools in order to control and predict gradual and sudden failures of cutting inserts.Damage control of cutting inserts is carried out by microscopic analysis. Deeper damage to the structure can be detected using the method of chemographic imaging. The proposed method is based on obtaining photographs of the oxidative reactions of materials of ultra-low concentrations occurring on the surface of solids under thermobaric loading.Before the moment of a sharp release of the energy of destruction, chemical processes of ultra-low concentrations are activated. Chemography allows to fix the zones where the incipient microcracks and microdefects are ready to actively develop, which can lead to the onset of macrodamage and failure to work.The chemographic image of the plate obtained as a result of the study is compared with the reference sample, as a result of which it is possible to assess the initial defect state of the material and predict the further period of the plate's operation.The criterion for the existing defects and imperfections in the structure is the change in the blackness index of the chemographic image, the minimum value of which indicates a minimum of structural defects and internal defects in the material under study.The results allow to propose a new method for controlling the surface of cutting plates, which can be easily implemented in any machine shop, which makes its application very promisin

Improving treatment of neurodevelopmental disorders: recommendations based on preclinical studies

Introduction: Neurodevelopmental disorders (NDDs) are common and severely debilitating. Their chronic nature and reliance on both genetic and environmental factors makes studying NDDs and their treatment a challenging task.Areas covered: Herein, the authors discuss the neurobiological mechanisms of NDDs, and present recommendations on their translational research and therapy, outlined by the International Stress and Behavior Society. Various drugs currently prescribed to treat NDDs also represent a highly diverse group. Acting on various neurotransmitter and physiological systems, these drugs often lack specificity of action, and are commonly used to treat multiple other psychiatric conditions. There has also been relatively little progress in the development of novel medications to treat NDDs. Based on clinical, preclinical and translational models of NDDs, our recommendations cover a wide range of methodological approaches and conceptual strategies.Expert opinion: To improve pharmacotherapy and drug discovery for NDDs, we need a stronger emphasis on targeting multiple endophenotypes, a better dissection of genetic/epigenetic factors or "hidden heritability," and a careful consideration of potential developmental/trophic roles of brain neurotransmitters. The validity of animal NDD models can be improved through discovery of novel (behavioral, physiological and neuroimaging) biomarkers, applying proper environmental enrichment, widening the spectrum of model organisms, targeting developmental trajectories of NDD-related behaviors and comorbid conditions beyond traditional NDDs. While these recommendations cannot be addressed all in once, our increased understanding of NDD pathobiology may trigger innovative cross-disciplinary research expanding beyond traditional methods and concepts

Synthesis and investigation of antioxidant activity of the dithiocarbamate derivatives of 9,10-anthracenedione

A variety of new derivatives of 9,10-anthracenedione with mono- and bisdithiocarbamate moiety in the non-catalytic conditions have been synthesized. The obtained compounds were screened for their antioxidant capacity using the cupric-reducing antioxidant capacity (CUPRAC) method. Three of the compounds showed CUPRAC-Trolox equivalent antioxidant capacity (TEAC) coefficients higher than trolox. Scavenging effects on reactive oxygen species (ROS), hydroxyl radical scavenging (HRS), hydrogen peroxide scavenging (HPS), and superoxide anion radical scavenging (SARS) of all synthesized compounds were determined. It was established that the results of HRS-, HPS-, and SARS-CUPRAC methods for three compounds are in accordance with TEAC-CUPRAC assay data