Design Study Conducted of a Stirred and Perfused Specimen Chamber for Culturing Suspended Cells on the International Space Station

A tightly knit numerical/experimental collaboration among the NASA Ames Research Center, NASA Glenn Research Center, and Payload Systems, Inc., was formed to analyze cell culturing systems for the International Space Station. The Cell Culture Unit is a facility scheduled for deployment on the space station by the Cell Culture Unit team at Ames. The facility houses multiple cell specimen chambers (CSCs), all of which have inlets and outlets to allow for replenishment of nutrients and for waste removal. For improved uniformity of nutrient and waste concentrations, each chamber has a pair of counterrotating stir bars as well. Although the CSC can be used to grow a wide variety of organic cells, the current study uses yeast as a model cell. Previous work identified groundbased protocols for perfusion and stirring to achieve yeast growth within the CSC that is comparable to that for yeast cultures grown in a shaken Ehrlenmeyer flask

Landscape natural resources management with soil and water conservation practices

United States Agency for International Developmen

The Influence of Management Support, Regulatory Frameworks and Technological Infrastructure on Information Security Culture in Government Institutions in Tanzania

This study delves into the intricate dynamics of information security culture within the Ministry of Finance in Tanzania, investigating the influential roles of leadership and management support, regulatory frameworks, and technological infrastructure. A cross-sectional research design was used with a quantitative research approach. A sample size of 84 respondents was drawn from a population of 503. Utilising a comprehensive survey that covers variables like commitment, compliance, and resource accessibility, the research reveals crucial findings. Leadership and management support foster a robust information security culture, directly impacting employee awareness, responsibility, and proactive security behaviours. Concurrently, compliance with regulatory frameworks emerges as pivotal, necessitating continuous training programs to ensure understanding and adherence. The study also underscores the profound impact of technological infrastructure on information security, emphasising the importance of accessible and reliable resources in fortifying the organisation's security posture. The research sheds light on the current information security culture within the Ministry of Finance. It furnishes actionable recommendations for sustained improvement, offering a valuable contribution to the broader discourse on cybersecurity within government institutions

Feasibility of the modified sequential organ function assessment score in a resource-constrained setting: a prospective observational study.

BackgroundSub-Saharan Africa has a great burden of critical illness with limited health care resources. We evaluated the feasibility and utility of the modified Sequential Organ Function Assessment (mSOFA) score in assessing morbidity and mortality in the National Referral Hospital's intensive care unit (ICU) for one year.MethodsWe conducted a prospective, observational cohort study on patients above 12 years of age admitted to the ICU at Mulago Hospital (Kampala, Uganda). All SOFA scores were determined at admission and at 48 h. We modified the SOFA score by replacing the PaO2/FiO2 ratio with SPO2/FiO2. The primary outcome was ICU mortality.ResultsThis ICU cohort of 118 patients had a mean age of 37 years and an ICU mortality rate of 46.6%. Non-survivors had higher initial (7.7 SD 3.8 vs. 5.5 SD 3.3; p = 0.007), mean (8.1 SD 3.9 vs 4.7 SD 2.6; p < 0.001) and highest mSOFA scores (9.4 SD 4.2 vs. 5.8 SD 3.2; p < 0.001), with an increase of 1.0 (SD 3.1) mSOFA on average after 48 h when compared to survivors (p < 0.001). The area under the receiver operating characteristic curves for each mSOFA category was: initial-0.68, mean-0.76, highest-0.76 and delta mSOFA-0.74. Multivariate logistic regression analysis showed no significant association between mSOFA scores and mortality.ConclusionOur results confirm that calculation of the mSOFA score is feasible for an ICU population in a resource-limited country. More data are needed to test for an association between mSOFA and mortality

The scourge of head injury among commercial motorcycle riders in Kampala; a preventable clinical and public health menace

Background: Trauma is an increasingly important cause of disease globally. Half of this trauma is from road traffic injuries with motorcycles contributing 21-58%. Low protective gear use, lack of regulation and weak traffic law enforcement contribute to unsafe nature of commercial motorcycles also known as “boda boda” in Uganda.Objectives: To determine the prevalence of protective gear use, the occurrence of head injury and the relationship between the two among commercial motorcycle riders in Kampala.Methods: Following ethical approval we recruited consecutive consenting participants to this analytical cross-sectional study. Data was collected using pretested interviewer administered questionnaires, double entered in Epidata and analyzed with STATA. Proportions and means were used to summarize data. Odds ratios were calculated for association between wearing helmets and occurrence and severity of head injury.Results: All 328 participants recruited were male. Of these, 18.6% used Protective gear and 71.1 % sustained head injury. Helmets protected users from head injury (OR 0.43, 95% CI, 0.23-0.8) and significantly reduced its severity when it occurred.Conclusion: Protective gear use was low, with high occurrence of head injury among commercial motorcycle riders in Uganda. More effective strategies are needed to promote protective gear use among Uganda’s commercial motorcycle riders.Keywords: Boda-boda, okada, commercial motorcycle, head injury, protective gear, helmet, Mulago hospital

Selection of Apps for Teaching Difficult Mathematics Topics: An Instrument to Evaluate Touch-Screen Tablet and Smartphone Mathematics Apps

Manipulatives—including the more recent touch-screen mobile device apps—belong to a broader network of learning tools. As teachers continue to search for learning materials that aid children to think mathematically, they are faced with a challenge of how to select materials that meet the needs of students. The profusion of virtual learning tools available via the Internet magnifies this challenge. What criteria could teachers use when choosing useful manipulatives? In this chapter, we share an evaluation instrument for teachers to use to evaluate apps. The dimensions of the instrument include: (a) the nature of the curriculum addressed in the app— emergent, adaptable or prescriptive, and relevance to current, high quality curricula—high, medium, low; (b) degree of actions and interactions afforded by the app as a learning tool— constructive, manipulable, or instructive interface; (c) the level of interactivity and range of options offered to the user —multiple or mono, or high, moderate or low; and, (d) the quality of the design features and graphics in the app—rich, high quality or impoverished, poor quality. Using these dimensions, researchers rated the apps on a three-level scale: Levels I, II, and III. Few apps were classified as Level III apps on selected dimensions. This evaluation instrument guides teachers when selecting apps. As well, the evaluation instrument guides developers in going beyond apps that are overly prescriptive, that focus on quizzes, that are text based, and include only surface aspects of using multi-modality in learning, to apps that are more aligned with emergent curricula, that focus also on conceptual understanding, and that utilize multiple, interactive representations of mathematics concepts

Landscape natural resources management using forage grasses and legume intercrops

United States Agency for International Developmen

Scientific Communication: Reporting on Mental Health Illness (MHI) in Kenya: how well are we doing?

No Abstrac

Spatial distribution and co-infection with urogenital and intestinal schistosomiasis among primary school children in Migori County, Kenya

Background: Schistosomiasis is the most prevalent helminthic infection in sub-Saharan Africa. School age children have the highest burden. Chronic schistosome infections in children can have irreversible effects lasting throughout adulthood.Objective: To determine the prevalence, intensity and distribution of urogenital and intestinal schistosomiasis among primary school children in Migori County, Kenya.Design: Descriptive cross-sectional study.Setting: Primary schools in Migori County, Kenya.Subjects: Children aged seven to fourteen years.Results: We enrolled 1,784 children with the ratio of female to males being 1:1. Their mean age was 11.4 years (standard deviation ± 1.6). The overall prevalence of infection was 26%, with urogenital and intestinal schistosomiasis being found in 9% and 20% of children, respectively. A trend of increasing prevalence with increasing age of children was observed. Boys had a 50% higher risk of being infected with schistosomiasis when compared to girls (adjusted odds ratio 1.5, 95% confidence interval 1.2-1.9). Intensities of schistosome infections did not vary significantly across gender and age. Nyatike Constituency had the highest prevalence of schistosomiasis (54%).Prevalence in schools ranged from 1.7 to 89%. Seven schools (23%) had a schistosomiasis prevalence of at least 50% while 12 (39%) schools had schistosomiasis prevalence of between 10 and 50%.Conclusion: Our study showed that schistosomiasis is endemic in the study area and represents a significant public health problem among school children. The area should be prioritised for interventions including mass deworming, public health education and sanitation improvement