Influence of platelets rich plasma (PRP) on the regeneration of skin conditions allergic dermatitis

Introduction: Modern approach to the problem of skin diseases characterized by the influence on the regeneration of tissues at the cellular level, leading to restoration of structure and function of the body as a whole. Aim: to examine the effects of PRP on regenerative properties of skin conditions allergic dermatitis. Materials and methods: Experiments were performed on female mice ICR, aged 3-4 months. PRP received by treating peripheral blood Machinery Smart Prep (Harvester Corp.). Animals were divided into two groups. The first group in plot area for 10 days rubbed potassium dichromate, then locally, intradermal injected into the alfected area PRP dose of 0.1 ml twice at intervals of 7 days. The second group of animals rubbed dichromate of potassium, during the same periodand then watched the self-healing skin. Animal deduced from the experiment on the 17th, 31st and 45th day. Conducted pathologic study involved skin using different methods of coloring material. Results: Macroscopically, visible damages to skin were not observed at the animals of the first group. The skin was covered with hair. In the morphological study of the skin revealed the preservation of its layer structure, satisfactory vascularization of the zone lesions, expressed basal layer, the structure of hair is not changed. Macroscopically, the skin of the animals from the second group was thin, with varying degrees of proliferation of connective tissue. Microscopically we detected acanthosis, small spongiosis, without bubbles, leukocyte infiltration of various degrees of severity, extension of epidermal outgrowths, areas of parakeratosis and phenomenon of acantholysis. Blood vessels had a small caliber. Conclusions: PRP promotes high quality and rapid reparative regeneration of skin, which helps preserve the morphological properties of tissue. The positive effect correction caused by several factors (cytokines and other biologically active substances), which enhance chemotaxis and proliferation of cellular elements in the lesion focus, and participate in the processes of adaptation

Periodontal disease related to oxidative stress

Th e study evaluates oxidative stress (OS) in patients with diff erent stages of periodontal disease (PD) and the infl uence of their smoking habits on OS. PD is related to connective tissue infl ammation, which leads to deterioration of the supporting structures of the tooth. OS is a condition characterised by overproduction of free radicals (FR), which may be involved in PD, causing periodontal tissue damage and other related diseases. Study patients' were grouped as I-non smokers (n=45) and II-smokers (n=45); and consisted of patients with 3 PD stages: mild (1), moderate (2) and severe (3). As a control group, 30 healthy subjects (all non smokers) with no signs of PD were selected. FR levels were determined by the D-Roms test, total antioxidant capacity (AOC) was determined by the OXY-adsorbent test, (Diacron, Italy) and lipid peroxidation (LP) was determined by the fl uorometric method with thiobarbituric acid and its end product, malonyldialdehyde (MDA). OS was found in the periodontal tissue and serum of PD patients, with the highest level of FR in the severe stage (3) in non smokers (p<0.05) as well as smokers (p<0.01); AOC showed decreasing values from mild (1) to severe stage (3) of PD for smokers (p<0.05). LP in serum showed the highest level in severe stage (3) in both groups i.e., non smokers and smokers (p<0.05) compared to controls. Based on the obtained results, we may conclude that PD is related to OS and may either be a cause or a trigger for more accelerated OS. Cigarette smoking increased FR production and is a serious factor exacerbating further tissue damage in PD. Th ese fi ndings may contribute to possible use of effi cient antioxidant agents as a preventive measure for PD and as a therapy for better disease outcome