ReSolVe project – Restoring optimal Soil functionality in degraded areas within organic Vineyards

In both conventional and organic European vineyards, it is quite common to have areas characterized by problems in vine health, grape production and quality. These problems are very often related to sub-optimal soil functionality, caused by an improper land preparation before vine plantation and/or management. Different causes for soil malfunctioning can include: poor organic matter content and plant nutrient availability (both major and trace elements); imbalance of some element ratios (Ca/Mg, K/Mg, P/Fe, and Fe/Mn); pH; water deficiency; soil compaction and/or scarce oxygenation. Fertility related problems can often be compensated in conventional settings with externally introduced fertilizers that are not permitted in organic vineyards. ReSolVe is a transnational and multidisciplinary research project aimed at testing the effects of selected agronomic strategies for restoring optimal soil functionality in degraded areas within organic vineyard. The term "degraded areas within vineyard" means areas showing reduced vine growth, disease resistance, grape yield and quality. These areas may have lost their soil functionality because of either an improper land preparation, or an excessive loss of soil organic matter and nutrients, erosion and/or compaction. The project, financed by Core-Organic plus program of the ERA-NET plus action (2015-2018), aims at identifying the main causes of the soil functionality loss and testing different organic recovering methods. The different restoring strategies will implement: i) compost, ii) green manure with winter legumes, iii) dry mulching with cover crops. The strategies will be tested according to their efficiency to improve: i) plant and roots growth and well-being; ii) grape yield and quality; iii) quality of soil ecosystem services and their stability over the years; iv) better express of the “terroir effect”, that is, the linkage of wine quality to the environmental characteristics of the cultivation site. The project involves 8 research groups in 6 different EU countries (Italy, France, Spain, Sweden, Slovenia, and Turkey), with experts from several disciplines, including soil science, ecology, microbiology, grapevine physiology, viticulture, and biometry. The experimental vineyards are situated in Italy (Chianti hills and Maremma plain, Tuscany), France (Bordeaux and Languedoc), Spain (La Rioja) and Slovenia (Primorska) for winegrape, and in Turkey (Adana and Mersin) for table grape. The restoration techniques and the monitoring methodologies developed and tested during the ReSolVe project will be described in specific final guidelines. The restoration techniques will be accessible for all the European farmers and will be low cost and environmental-friendly. A protocol of analyses and measurements between the all partners will allow an effective and comparable monitoring of vineyard ecosystemic functioning in European countries

Similar bacterial signatures in the gut microbiota of type 1 and type 2 diabetes patients and its association with G protein-coupled receptor 41 and 43 gene expression

Purpose There are conflicting reports regarding the abundance of short-chain fatty acids producing bacteria in the gut microbiota in patients with type 1 and type 2 diabetes. We aimed to determine the amount of Akkermansia muciniphila, Anaerobutyricum hallii, Bifidobacterium adolescentis, Bifidobacterium longum, Collinsella aerofaciens, Faecalibacterium prausnitzii, Lacticaseibacillus rhamnosus, and Parabacteroides distasonis in the gut microbiota in patients with type1 and type2 diabetes, compared with the healthy controls and analyze the correlation between the gene expression levels of two short-chain fatty acids receptors GPR41 and GPR43. Methods Forty type 1, 40 type 2 stool and blood samples of diabetes patients, and 40 healthy control samples were studied. DNA and RNA were extracted, and bacteria were detected using a Microbial DNA qPCR Assay kit. Gene expressions were detected with GPR41 and GPR43 primers via in-house qPCR. Results Compared with healthy controls, B.longum and F.prausnitzii abundance were significantly decreased in patients with type1 and type2 diabetes, A.hallii abundance was increased in patients with type1 and decreased in type2 diabetes contrarily A.muciniphila abundance was decreased in patients with type1 and increased in type2 diabetes. GPR43 gene expression was upregulated in both patients group, however GPR41 was upregulated only in patients with type2 diabetes. Conclusions Elevated B. longum and F. prausnitzii abundances were detected in the gut microbiota of patients with type1 and type2 diabetes and compared with healthy controls. B. longum and F.prausnitzii abundances were also correlated with the GPR43 gene expression level in type1 diabetes patients. Extensive studies determining bacteria producing short-chain fatty acids in gut microbiota, and their contribution in the pathogenesis of diabetes, are needed to understand better the mechanism of these diseases

Firmicutes/Bacteroidetes Ratio in the Gut Microbiota and IL-1?, IL-6, IL-8, TLR2, TLR4, TLR5 Gene Expressions in Type 2 Diabetes

Background: The aim of this study was to determine the Firmicutes/Bacteroidetes ratio in the gut microbiota and IL-1?, IL-6, IL-8, TLR2, TLR4 and TLR5 gene expression levels in the blood of adult type 2 diabetes (T2D) patients and compare it with that of adult nondiabetic healthy controls (HC). Methods: Between May 2016 and April 2017, 99 T2D patients and 99 HCs were enrolled in the study. Bacteroidetes and Firmicutes levels were assessed from stool sample DNA and IL-1?, IL-6, IL-8, TLR2, TLR4, and TLR5 gene expression levels assesed from blood sample RNA via qPCR from both T2D patients and healthy controls. Results: The Firmicutes/Bacteroidetes ratio detected in the stool of type 2 diabetes patients was found to be higher with a statistically significant difference (p < 0.0001). Gene expression levels of IL-1?, IL-6, IL-8, TLR2, TLR4, and TLR5 were found to be upregulated. Conclusions: The highest upregulation was detected in IL-6 with 11 fold in T2D patients comparing with HCs. F/B ratio and gene expression levels were elevated in T2D patients. Firmicutes were positively correlated with studied gene expressions. A better understanding of the complex interaction between gut microbiota, environment, and diabetes will allow for more effective prevention and treatment strategies for T2D. © 2022 Verlag Klinisches Labor GmbH. All rights reserved.Istanbul Üniversitesi: 3748, TSA-2016-3748We are grateful for the support of Istanbul University. This research was supported by the Scientific Research Fund of Istanbul University and Istanbul University - Cerrahpasa, Project and Grant No.: 3748 (TSA-2016-3748)

Interferon-gamma assays for the diagnosis of tuberculosis infection before using tumour necrosis factor-alpha blockers

OBJECTIVES: Patients who receive tumour necrosis factor-alpha (TNF-alpha) blockers are mostly immunosuppressed. A study was performed to investigate whether an interferon-gamma (IFN-gamma) assay could rep-resent an alternative approach to the tuberculin skin test (TST) for the diagnosis of latent tuberculosis infection (LTBI) in these patients

Identification of susceptibility loci in IL6, RPS9/LILRB3, and an intergenic locus on chromosome 21q22 in takayasu arteritis in a genome-wide association study

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/111261/1/art39035.pd