The Effect of Different TALP-Components on the Progressive Motility of Bovine Spermatozoa During the Centrifugation and Incubation Stages as a Prerequisite for In Vitro Fertilisation

In the present study the effect of different Modified Tyrode's media-TALP and different sperm concentrations on the progressive motility of the spermatozoa was examined. TALP medium containing high levels of lactate, NaHCO3 and Hepes provided the best environment for the spermatozoa during the swim-up first and second centrifugation stages. However the medium, in which lactate was the primary nutritional source, gave the best results during CO2 incubation. Treatments of spermatozoa in an air environment gave much poor results than treatments in a CO2 environment. Sperm motility was optimal when the sperm concentration was around 20x10

ATMOSPHERIC DEPOSITION IN MATURE FIR (Abies nordmanniana) STANDS IN ALADAGI-BOLU, TURKEY

The aim of this study was to determine the atmospheric deposition in fir forests (Abies nordmannianna (Steven)) in the Bolu Aladag region. The study area selected was located in the mountainous Aladag region stretching along the south of Bolu Province in the Western Black Sea Region of Turkey. Four elevation zones (1000, 1200, 1400, and 1600 m above sea level) were designated on the closed-canopy fir forest-dominated northern slopes of the mountain. Sampling plots were established for each elevation zone, eight on randomly located sites under the canopy and three on adjacent open sites. For each elevation zone, open site, throughfall, and stemflow deposition samples were collected between April and November for two years (2013-2014). Chemical analysis of the samples was performed in the ICP laboratory located within the Aegean Forestry Research Institute in Izmir. One-way ANOVA was carried out for each variable to determine the differences in the sampling plots. Sulfate, nitrogen, potassium, and calcium were the main ions deposited in the fir forests in the 1000-1600-m elevation zones in Aladag. The acid neutralization capacity of the deposition samples collected in the open site plots was high for all elevation zones.Duzce University BAP programDuzce UniversityThis research was funded by the Duzce University BAP program through the project entitled Bolu-Aladag, Uludag Goknari (Abies bormulleriana Mattf.) Ormanlarinda Atmosferik Cokelme [Atmospheric deposition in mature fir (Abies bormulleriana Mattf.) stands in Aladag-Bolu] and numbered as DUBAP 02.02.213.WOS:0006043446000112-s2.0-8509850539

ATMOSPHERIC DEPOSITION IN MATURE FIR (Abies nordmanniana) STANDS IN ALADAGI-BOLU, TURKEY

The aim of this study was to determine the atmospheric deposition in fir forests (Abies nordmannianna (Steven)) in the Bolu Aladag region. The study area selected was located in the mountainous Aladag region stretching along the south of Bolu Province in the Western Black Sea Region of Turkey. Four elevation zones (1000, 1200, 1400, and 1600 m above sea level) were designated on the closed-canopy fir forest-dominated northern slopes of the mountain. Sampling plots were established for each elevation zone, eight on randomly located sites under the canopy and three on adjacent open sites. For each elevation zone, open site, throughfall, and stemflow deposition samples were collected between April and November for two years (2013-2014). Chemical analysis of the samples was performed in the ICP laboratory located within the Aegean Forestry Research Institute in Izmir. One-way ANOVA was carried out for each variable to determine the differences in the sampling plots. Sulfate, nitrogen, potassium, and calcium were the main ions deposited in the fir forests in the 1000-1600-m elevation zones in Aladag. The acid neutralization capacity of the deposition samples collected in the open site plots was high for all elevation zones.Duzce University BAP programDuzce UniversityThis research was funded by the Duzce University BAP program through the project entitled Bolu-Aladag, Uludag Goknari (Abies bormulleriana Mattf.) Ormanlarinda Atmosferik Cokelme [Atmospheric deposition in mature fir (Abies bormulleriana Mattf.) stands in Aladag-Bolu] and numbered as DUBAP 02.02.213.WOS:0006043446000112-s2.0-8509850539

XIST expression in human oocytes and preimplantation embryos.

During mouse preimplantation development, the exclusive expression of the Xist gene from the paternally inherited allele is thought to play a role in the inactivation of the paternally inherited X chromosome in the extra-embryonic cell lineages of the developing female embryo. Recently, inactivation of the paternally inherited X chromosome has also been shown to occur in the extraembryonic cell lineages of the human female conceptus. In this paper, we determine whether the pattern of XIST expression in human preimplantation embryos is similarly correlated with paternal X inactivation. We developed procedures sensitive to the single cell, for the simultaneous analysis of XIST and HPRT expression and of sexing, initially using human fibroblast cells. Application of these procedures to human cleavage-stage embryos derived by in vitro fertilization revealed a pattern of XIST expression different from that in the mouse. Transcripts of the XIST gene were detected as early as the 1-cell zygote and, with increasing efficiency, through to the 8-cell stage of preimplantation development. In addition, transcripts of XIST were detected in both male (hence from the maternally inherited allele) and female preimplantation embryos. This pattern of expression is not consistent with a role for the early expression of the XIST gene in the choice of paternal X inactivation in the extraembryonic cell lineages of the developing human embryo

Viability of cloned bovine embryos after one or two cycles of nuclear transfer and in vitro culture

We described an exclusively in vitro procedure for cloning and recloning bovine embryos. Embryos obtained by IVM/IVF/IVC developed to the morula stage were used as blastomere donors in cunjunction with IVM recipient oocytes. Reconstructed embryos were developed in vitro in co-culture using bovine oviductal epithelial cells. The resulting morulae were used as donors for recloning under the same experimental conditions. No significant difference was observed between cloning and recloning in terms of development (rates of blastocysts: 12.9 versus 14.9%), in the number of nuclei per blastocyst (63.8 versus 49.1), or in pregnancy rates (35.7 versus 33.3%). The high variability observed between replicates and the correlation between results in first and second cycle nuclear transfer may suggest an inherant potential of individual donor embryos to support development by clonin