Perceived Barriers Preventing and Treating HIV/AIDS among Public Health Workers in Florida

In 2015, Florida moved from being ranked second to first nationally in total number of HIV diagnoses. To combat this statistic, public health workers were interviewed to understand perceived perspectives about available resources and practicable solutions to barriers that may inhibit the use of testing and treatment services to reduce overall health disparities and inequalities among individuals with HIV/AIDS. Ten public health workers from rural counties in Florida were interviewed, and then qualitatively analyzed using the constant comparison method. Public health workers found that multiple barriers, lack of education and knowledge of resources available among health workers, and a need for continuing education on HIV/AIDS have an impact on how preventive services and treatment are carried out. Along with highlighting key issues among public health workers, in this paper, we hope to provide feasible solutions at a time where public health funding is decreasing

Dominating the Antarctic environment: bryophytes in a time of change

Polar ecosystems, and particularly Antarctica, are one of the few environs in which bryophytes dominate the flora. Their success in these regions is due to bryophytes’ ability to withstand an array of harsh conditions through their poikilohydric lifestyle. However, the unique conditions that allow bryophytes to proliferate over other forms of vegetation also create considerable limitations to growth and photosynthetic activity. High latitude areas are already experiencing some of the most pronounced and rapid climatic change, especially in the Arctic, the Sub-Antarctic Islands and Maritime Antarctica, and these are predicted to continue over the next century. This climatic change is already impacting the flora of the polar regions both via direct and/or indirect impacts on plant species. Water availability and temperature are undoubtedly the most influential factors that determine bryophyte productivity in the Antarctic, but the ozone hole is also having an impact either directly via increased ultraviolet-B radiation and/or indirectly through the increasing wind speeds associated with ozone depletion. In a time of shifting climate the dominance of bryophytes in these regions may be threatened

A New Semantic Theory of Natural Language

Formal Semantics and Distributional Semantics are two important semantic frameworks in Natural Language Processing (NLP). Cognitive Semantics belongs to the movement of Cognitive Linguistics, which is based on contemporary cognitive science. Each framework could deal with some meaning phenomena, but none of them fulfills all requirements proposed by applications. A unified semantic theory characterizing all important language phenomena has both theoretical and practical significance; however, although many attempts have been made in recent years, no existing theory has achieved this goal yet. This article introduces a new semantic theory that has the potential to characterize most of the important meaning phenomena of natural language and to fulfill most of the necessary requirements for philosophical analysis and for NLP applications. The theory is based on a unified representation of information, and constructs a kind of mathematical model called cognitive model to interpret natural language expressions in a compositional manner. It accepts the empirical assumption of Cognitive Semantics, and overcomes most shortcomings of Formal Semantics and of Distributional Semantics. The theory, however, is not a simple combination of existing theories, but an extensive generalization of classic logic and Formal Semantics. It inherits nearly all advantages of Formal Semantics, and also provides descriptive contents for objects and events as fine-gram as possible, descriptive contents which represent the results of human cognition

Genome-wide characterization of cytosine-specific 5-hydroxymethylation in normal breast tissue.

Despite recent evidence that 5-hydroxymethylcytosine (5hmC) possesses roles in gene regulation distinct from 5-methylcytosine (5mC), relatively little is known regarding the functions of 5hmC in mammalian tissues. To address this issue, we utilized an approach combining both paired bisulfite (BS) and oxidative bisulfite (oxBS) DNA treatment, to resolve genome-wide patterns of 5hmC and 5mC in normal breast tissue from disease-free women. Although less abundant than 5mC, 5hmC was differentially distributed, and consistently enriched among breast-specific enhancers and transcriptionally active chromatin. In contrast, regulatory regions associated with transcriptional inactivity, such as heterochromatin and repressed Polycomb regions, were relatively depleted of 5hmC. Gene regions containing abundant 5hmC were significantly associated with lactate oxidation, immune cell function, and prolactin signaling pathways. Furthermore, genes containing abundant 5hmC were enriched among those actively transcribed in normal breast tissue. Finally, in independent data sets, normal breast tissue 5hmC was significantly enriched among CpG loci demonstrated to have altered methylation in pre-invasive breast cancer and invasive breast tumors. Primarily, our findings identify genomic loci containing abundant 5hmC in breast tissues and provide a genome-wide map of nucleotide-level 5hmC in normal breast tissue. Additionally, these data suggest 5hmC may participate in gene regulatory programs that are dysregulated during breast-related carcinogenesis

A data assimilation approach to last millennium temperature field reconstruction using a limited high-sensitivity proxy network

The authors acknowledge support from the Climate Program Office of the National Oceanographic and Atmospheric Administration (NOAA grants NA18OAR4310420 to KJA, NA18OAR4310426 to JEG and FZ, and NA18OAR4310422 to GJH). GJH also acknowledges support from the NSF through grant AGS–1702423. JMK was partially supported by NSF grant AGS-1803946. JET and JMK acknowledge support from NSF grant #AGS-1602301 and Heising655 Simons Foundation grant #2016-05.We use the Northern Hemisphere Tree-Ring Network Development (NTREND) tree-ring database to examine the effects of using a small, highly-sensitive proxy network for paleotemperature data assimilation over the last millennium. We first evaluate our methods using pseudo-proxy experiments. These indicate that spatial assimilations using this network are skillful in the extratropical Northern Hemisphere and improve on previous NTREND reconstructions based on Point-by-Point regression. We also find our method is sensitive to climate model biases when the number of sites becomes small. Based on these experiments, we then assimilate the real NTREND network. To quantify model prior uncertainty, we produce 10 separate reconstructions, each assimilating a different climate model. These reconstructions are most dissimilar prior to 1100 CE, when the network becomes sparse, but show greater consistency as the network grows. Temporal variability is also underestimated before 1100 CE. Our assimilation method produces spatial uncertainty estimates and these identify treeline North America and eastern Siberia as regions that would most benefit from development of new millennial-length temperature-sensitive tree-ring records. We compare our multi-model mean reconstruction to five existing paleo-temperature products to examine the range of reconstructed responses to radiative forcing. We find substantial differences in the spatial patterns and magnitudes of reconstructed responses to volcanic eruptions and in the transition between the Medieval epoch and Little Ice Age. These extant uncertainties call for the development of a paleoclimate reconstruction intercomparison framework for systematically examining the consequences of proxy network composition and reconstruction methodology and for continued expansion of tree-ring proxy networks.Publisher PDFPeer reviewe

The non-coding landscape of head and neck squamous cell carcinoma.

Head and neck squamous cell carcinoma (HNSCC) is an aggressive disease marked by frequent recurrence and metastasis and stagnant survival rates. To enhance molecular knowledge of HNSCC and define a non-coding RNA (ncRNA) landscape of the disease, we profiled the transcriptome-wide dysregulation of long non-coding RNA (lncRNA), microRNA (miRNA), and PIWI-interacting RNA (piRNA) using RNA-sequencing data from 422 HNSCC patients in The Cancer Genome Atlas (TCGA). 307 non-coding transcripts differentially expressed in HNSCC were significantly correlated with patient survival, and associated with mutations in TP53, CDKN2A, CASP8, PRDM9, and FBXW7 and copy number variations in chromosomes 3, 5, 7, and 18. We also observed widespread ncRNA correlation to concurrent TP53 and chromosome 3p loss, a compelling predictor of poor prognosis in HNSCCs. Three selected ncRNAs were additionally associated with tumor stage, HPV status, and other clinical characteristics, and modulation of their expression in vitro reveals differential regulation of genes involved in epithelial-mesenchymal transition and apoptotic response. This comprehensive characterization of the HNSCC non-coding transcriptome introduces new layers of understanding for the disease, and nominates a novel panel of transcripts with potential utility as prognostic markers or therapeutic targets

Leveraging Public-Private Partnerships During COVID-19: Providing Virtual Field Opportunities for Student Learners and Addressing Social Isolation in Older Adults

While preventive and management measures are important to mitigate the spread of COVID-19, strategies like social distancing can have devastating effects on older adults who are already at risk for social isolation and loneliness. In response, two Colleges of Health Professions (Social Work and Nursing) at a large public University leveraged a partnership with a national health and wellbeing company to address social isolation and loneliness in Houston area older adults during the COVID-19 pandemic. This intergenerational linkage initiative involved 707 older adults and 177 graduate social work and nursing students. This study describes the process of developing a virtual educational opportunity for students while also meeting the needs of vulnerable older adults in Houston, the third largest, and one of the most diverse cities in the U.S. Findings include student/learner outcomes, as well as self-reported improvements in loneliness scores, and unhealthy physical and mental health days among enrolled older adults

Extensive production of Neospora caninum tissue cysts in a carnivorous marsupial succumbing to experimental neosporosis

Experimental infections of Sminthopsis crassicaudata, the fat-tailed dunnart, a carnivorous marsupial widely distributed throughout the arid and semi-arid zones of Australia, show that this species can act as an intermediate host for Neospora caninum. In contrast to existing models that develop relatively few N. caninum tissue cysts, dunnarts offer a new animal model in which active neosporosis is dominated by tissue cyst production. The results provide evidence for a sylvatic life cycle of N. caninum in Australia between marsupials and wild dogs. It establishes the foundation for an investigation of the impact and costs of neosporosis to wildlife