The Magnetized Disk-Halo Transition Region of M51

An excellent laboratory for studying large scale magnetic fields is the grand de- sign face-on spiral galaxy M51. Due to wavelength-dependent Faraday depolarization, linearly polarized synchrotron emission at different radio frequencies gives a picture of the galaxy at different depths: Observations at L-band (1-2 GHz) probe the halo region while at C- and X- band (4-8 GHz) the linearly polarized emission probe the disk region of M51. We present new observations of M51 using the Karl G. Jansky Very Large Array (VLA) at S-band (2-4 GHz), where previously no polarization observations existed, to shed new light on the transition region between the disk and the halo. We discuss a model of the depolarization of synchrotron radiation in a multilayer magneto-ionic medium and compare the model predictions to the multi-frequency polarization data of M51 between 1-8GHz. The new S-band data are essential to distinguish between different models. Our study shows that the initial model parameters, i.e. the total reg- ular and turbulent magnetic field strengths in the disk and halo of M51, need to be adjusted to successfully fit the models to the data.Comment: 4 Pages, 3 Figures, Conference Proceeding to IAU Focus Meeting 8: New Insights in Extragalactic Magnetic Field

Localization and Characterization of STRO-1+ Cells in the Deer Pedicle and Regenerating Antler

The annual regeneration of deer antlers is a unique developmental event in mammals, which as a rule possess only a very limited capacity to regenerate lost appendages. Studying antler regeneration can therefore provide a deeper insight into the mechanisms that prevent limb regeneration in humans and other mammals, and, with regard to medical treatments, may possibly even show ways how to overcome these limitations. Traditionally, antler regeneration has been characterized as a process involving the formation of a blastema from de-differentiated cells. More recently it has, however, been hypothesized that antler regeneration is a stem cell-based process. Thus far, direct evidence for the presence of stem cells in primary or regenerating antlers was lacking. Here we demonstrate the presence of cells positive for the mesenchymal stem cell marker STRO-1 in the chondrogenic growth zone and the perivascular tissue of the cartilaginous zone in primary and regenerating antlers as well as in the pedicle of fallow deer (Dama dama). In addition, cells positive for the stem cell/progenitor cell markers STRO-1, CD133 and CD271 (LNGFR) were isolated from the growth zones of regenerating fallow deer antlers as well as the pedicle periosteum and cultivated for extended periods of time. We found evidence that STRO-1+ cells isolated from the different locations are able to differentiate in vitro along the osteogenic and adipogenic lineages. Our results support the view that the annual process of antler regeneration might depend on the periodic activation of mesenchymal progenitor cells located in the pedicle periosteum. The findings of the present study indicate that not only limited tissue regeneration, but also extensive appendage regeneration in a postnatal mammal can occur as a stem cell-based process

The magnetized disk-halo transition region of M 51

The grand-design face-on spiral galaxy M 51 is an excellent laboratory for studying magnetic fields in galaxies. Due to wavelength-dependent Faraday depolarization, linearly polarized synchrotron emission at different radio frequencies yields a picture of the galaxy at different depths: observations in the L-band (1-2 GHz) probe the halo region, while at 4.85 GHz (C-band) and 8.35 GHz (X-band), the linearly polarized emission mostly emerges from the disk region of M 51. We present new observations of M 51 using the Karl G. Jansky Very Large Array at the intermediate frequency range of the S-band (2-4 GHz), where previously no high-resolution broadband polarization observations existed, to shed new light on the transition region between the disk and the halo. We present the S-band radio images of the distributions of the total intensity, polarized intensity, degree of polarization, and rotation measure (RM). The RM distribution in the S-band shows a fluctuating pattern without any apparent large-scale structure. We discuss a model of the depolarization of synchrotron radiation in a multi-layer magneto-ionic medium and compare the model predictions to the multi-frequency polarization data of M 51 between 1-8 GHz. The model makes distinct predictions of a two-layer (disk-halo) and three-layer (far-side halo "disk"near-side halo) system. Since the model predictions strongly differ within the wavelength range of the S-band, the new S-band data are essential for distinguishing between the different systems. A two-layer model of M 51 is preferred. The parameters of the model are adjusted to fit to the data of polarization fractions in a few selected regions. In three spiral arm regions, the turbulent field in the disk dominates with strengths between 18 μG and 24 μG, while the regular field strengths are 8 - 16 μG. In one inter-arm region, the regular field strength of 18 μG exceeds that of the turbulent field of 11 μG. The regular field strengths in the halo are 3 - 5 μG. The observed RMs in the disk-halo transition region are probably dominated by tangled regular fields, as predicted from models of evolving dynamos, and/or vertical fields, as predicted from numerical simulations of Parker instabilities or galactic winds. Both types of magnetic fields have frequent reversals on scales similar to or larger than the beam size (∼550 pc) that contribute to an increase of the RM dispersion and to distortions of any large-scale pattern of the regular field. Our study devises new ways of analyzing and interpreting broadband multi-frequency polarization data that will be applicable to future data from, for example, the Square Kilometre Array

The magnetized disk-halo transition region of M51

The grand-design face-on spiral galaxy M51 is an excellent laboratory for studying magnetic fields in galaxies. We present new observations of M51 using the VLA at the frequency range of S-band (2-4GHz), to shed new light on the transition region between the disk and halo. We present images of the distributions of the total intensity, polarized intensity, degree of polarization, and rotation measure (RM). The RM distribution in S-band shows a fluctuating pattern without any apparent large-scale structure. We discuss a model of the depolarization of synchrotron radiation in a multi-layer magneto-ionic medium and compare the model predictions to the polarization data of M51 between 1-8GHz. Since the model predictions strongly differ within the wavelength range of the S-band, the new data are essential. The parameters of the model are adjusted to fit to the data of polarization fractions in a few selected regions. In three spiral arm regions, the turbulent field in the disk dominates with strengths between 18muG and 24muG, while the regular field strengths are 8-16muG. In one inter-arm region, the regular field strength of 18muG exceeds that of the turbulent field of 11muG. The regular field strengths in the halo are 3-5muG. The observed RMs in the disk-halo transition region are probably dominated by tangled regular fields, as predicted from models of evolving dynamos, and/or vertical fields, as predicted from numerical simulations of Parker instabilities or galactic winds. Both types of magnetic fields have frequent reversals on scales similar to or larger than the beam size (550pc) that contribute to an increase of the RM dispersion and to distortions of any large-scale pattern of the regular field. Our study devises new ways of analyzing and interpreting broadband multi-frequency polarization data that will be applicable to future data from, for example, the Square Kilometre Array.Comment: Accepted for publication in A&

Gene expression profiling reveals a conserved microglia signature in larval zebrafish

International audienceMicroglia are the resident macrophages of the brain. Over the past decade, our understanding of the function of these cells has significantly improved. Microglia do not only play important roles in the healthy brain but are involved in almost every brain pathology. Gene expression profiling allowed to distinguish microglia from other macro-phages and revealed that the full microglia signature can only be observed in vivo. Thus, animal models are irreplaceable to understand the function of these cells. One of the popular models to study microglia is the zebrafish larva. Due to their optical transparency and genetic accessibility, zebrafish larvae have been employed to understand a variety of microglia functions in the living brain. Here, we performed RNA sequencing of larval zebrafish microglia at different developmental time points: 3, 5, and 7 days post fertilization (dpf). Our analysis reveals that larval zebrafish microglia rapidly acquire the core microglia signature and many typical microglia genes are expressed from 3 dpf onwards. The majority of changes in gene expression happened between 3 and 5 dpf, suggesting that differentiation mainly takes place during these days. Furthermore, we compared the larval microglia transcriptome to published data sets of adult zebrafish microglia, mouse microglia, and human microglia. Larval microglia shared a significant number of expressed genes with their adult counterparts in zebrafish as well as with mouse and human microglia. In conclusion, our results show that larval zebrafish microglia mature rapidly and express the core microglia gene signature that seems to be conserved across species. K E Y W O R D S brain, evolution, microglia, RNA sequencing, transcriptome, zebrafis

Effect of Different Factors on Proliferation of Antler Cells, Cultured In Vitro

Antlers as a potential model for bone growth and development have become an object of rising interest. To elucidate processes explaining how antler growth is regulated, in vitro cultures have been established. However, until now, there has been no standard method to cultivate antler cells and in vitro results are often opposite to those reported in vivo. In addition, many factors which are often not taken into account under in vitro conditions may play an important role in the development of antler cells. In this study we investigated the effects of the antler growth stage, the male individuality, passaged versus primary cultures and the effect of foetal calf serum concentrations on proliferative potential of mixed antler cell cultures in vitro, derived from regenerating antlers of red deer males (Cervus elaphus). The proliferation potential of antler cells was measured by incorporation of 3H thymidine. Our results demonstrate that there is no significant effect of the antler growth stage, whereas male individuality and all other examined factors significantly affected antler cell proliferation. Furthermore, our results suggest that primary cultures may better represent in vivo conditions and processes occurring in regenerating antlers. In conclusion, before all main factors affecting antler cell proliferation in vitro will be satisfactorily investigated, results of in vitro studies focused on hormonal regulation of antler growth should be taken with extreme caution

The widest frequency radio relic spectra: observations from 150 MHz to 30 GHz

Galaxie