多能性幹細胞における転写不均一性の制御

筑波大学 (University of Tsukuba)201

FIRST - Flexible interactive retrieval SysTem for visual lifelog exploration at LSC 2020

Lifelog can provide useful insights of our daily activities. It is essential to provide a flexible way for users to retrieve certain events or moments of interest, corresponding to a wide variation of query types. This motivates us to develop FIRST, a Flexible Interactive Retrieval SysTem, to help users to combine or integrate various query components in a flexible manner to handle different query scenarios, such as visual clustering data based on color histogram, visual similarity, GPS location, or scene attributes. We also employ personalized concept detection and image captioning to enhance image understanding from visual lifelog data, and develop an autoencoderlike approach for query text and image feature mapping. Furthermore, we refine the user interface of the retrieval system to better assist users in query expansion and verifying sequential events in a flexible temporal resolution to control the navigation speed through sequences of images

Natural Radioactivity in Commonly Building Materials Used in Vietnam - 11255

ABSTRACT Naturally occurring radionuclides in building materials contribute to external and internal exposure and that is necessary to be investigated. In the research, 213 samples of 11 different kinds of commonly used structural and covering building materials were collected from housing and other building construction sites and from suppliers in Vietnam to measure the natural radioactivity of Ra-226, Th-232 and K-40. The measurements were carried out using low background gamma-ray spectrometry with HPGe detector. The specific activities of the different building materials varied from 0.18 -395.28, 0.10 -266.52 and 0.76 -2006.78 Bq/kg with the average values of 52.09, 55.70 and 593.5 Bq/kg for Ra-226, Th-232 and K-40, respectively. The obtained data were compared with the corresponding reported data of other countries. The activity concentration index and the annual effective dose were evaluated to assess the potential radiological hazard associated with these building materials. The results showed that the enhanced concentration values were sometimes felling into granite tiles, especially imported granite tiles. These obtained data aimed to enlarge the database on natural radioactivity in commonly building materials used in Vietnam and to support technical aspects in hazard exposure reduction

HOXA9 Reprograms the Enhancer Landscape to Promote Leukemogenesis

Aberrant expression of HOXA9 is a prominent feature of acute leukemia driven by diverse oncogenes. Here we show that HOXA9 overexpression in myeloid and B progenitor cells leads to significant enhancer reorganizations with prominent emergence of leukemia-specific de novo enhancers. Alterations in the enhancer landscape lead to activation of an ectopic embryonic gene program. We show that HOXA9 functions as a pioneer factor at de novo enhancers and recruits CEBPα and the MLL3/MLL4 complex. Genetic deletion of MLL3/MLL4 blocks histone H3K4 methylation at de novo enhancers and inhibits HOXA9/MEIS1-mediated leukemogenesis in vivo. These results suggest that therapeutic targeting of HOXA9-dependent enhancer reorganization can be an effective therapeutic strategy in acute leukemia with HOXA9 overexpressio

Comprehensive Identification of Krüppel-Like Factor Family Members Contributing to the Self-Renewal of Mouse Embryonic Stem Cells and Cellular Reprogramming.

Pluripotency is maintained in mouse embryonic stem (ES) cells and is induced from somatic cells by the activation of appropriate transcriptional regulatory networks. Krüppel-like factor gene family members, such as Klf2, Klf4 and Klf5, have important roles in maintaining the undifferentiated state of mouse ES cells as well as in cellular reprogramming, yet it is not known whether other Klf family members exert self-renewal and reprogramming functions when overexpressed. In this study, we examined whether overexpression of any representative Klf family member, such as Klf1-Klf10, would be sufficient for the self-renewal of mouse ES cells. We found that only Klf2, Klf4, and Klf5 produced leukemia inhibitory factor (LIF)-independent self-renewal, although most KLF proteins, if not all, have the ability to occupy the regulatory regions of Nanog, a critical Klf target gene. We also examined whether overexpression of any of Klf1-Klf10 would be sufficient to convert epiblast stem cells into a naïve pluripotent state and found that Klf5 had such reprogramming ability, in addition to Klf2 and Klf4. We also delineated the functional domains of the Klf2 protein for LIF-independent self-renewal and reprogramming. Interestingly, we found that both the N-terminal transcriptional activation and C-terminal zinc finger domains were indispensable for this activity. Taken together, our comprehensive analysis provides new insight into the contribution of Klf family members to mouse ES self-renewal and cellular reprogramming

EVALUATION AND PREDICTION OF LAND USE, AND LAND COVER CHANGES USING REMOTE SENSING AND CA-ANN MODEL IN HUONG HOA DISTRICT, QUANG TRI PROVINCE

Evaluation of land use and land cover change (LULC) is necessary for densely vegetated areas like Huong Hoa district, Quang Trị province. It is a basis for sustainable development strategies. Therefore, the study aims to evaluate the LULC change in the 10-year period of 2013–2023 by using Landsat 8 satellite image data with the Maximum Likelihood Classification method and to predict future LULC changes. The LULC maps for 2013, 2018, and 2023 are accurate, with Kappa coefficients from 0.82 to 0.85. In the period of 2013–2023, the dense vegetation area tended to decrease by 1.4%. The decrease was mainly due to the transition to sparse vegetation cover. Bare land increased by 0.5%, and the built-up area decreased by 0.6%. Meanwhile, the water body changed slightly. The prediction of LULC change with the CA-ANN model in the QGIS MOLUSCE plugin is based on the history of LULC change and two spatial variables: DEM and distance to the road. The accuracy of the CA-ANN model is satisfactory, with an overall accuracy of 86% and a Kappa coefficient of 0.76. In the simulated LULC of 2033, dense vegetation is predicted to keep a higher decrease by 2% in the area in comparison with the LULC of 2023. Sparse vegetation steadily increased by 1.3% over the subsequent 10 years. Similarly, the built-up area, water body, and bare land extended slightly by 0.5, 0.1, and 0.1%, respectively. The CA-ANN model in the QGIS MOLUSCE plugin is suitable for the simulated LULC changes for the studied area

Chimera formation with iPSCs reprogrammed from EpiSCs.

<p>(A) Chimeras at E9.5 generated from two independent iPSC lines (#87 and 89). (B) Chimera and its genital ridge at E13.5 generated from iPSC line #89. Scale bar: 1 mm.</p