Hurdles to the Court: The Doctrine of Standing under Statutory Violations

Standing is a precondition for any suit brought in federal court. This Commentary analyzes a Supreme Court case, Spokeo, Inc. v. Robins, which will address whether a violation of a federal statute grants a plaintiff standing to sue. The Author argues that such a violation is sufficient for establishing standing because the plaintiff suffered an injury-in-fact which the legisture intended to prevent. That harm is both traceable to the violation and redressible by statute. Thus, the requisite elements of constitutional standing exist in this case. Such a holding follows from existing standing jurisprudence and ensures that plaintiffs can have their day in court

Molecular electronics exploiting sharp structure in the electrode density-of-states. Negative differential resistance and Resonant Tunneling in a poled molecular layer on Al/LiF electrodes

Density-functional calculations are used to clarify the role of an ultrathin LiF layer on Al electrodes used in molecular electronics. The LiF layer creates a sharp density of states (DOS), as in a scanning-tunneling microscope (STM) tip. The sharp DOS, coupled with the DOS of the molecule leads to negative differential resistance (NDR). Electron transfer between oriented molecules occurs via resonant tunneling. The I-V characteristic for a thin-film of tris (8-hydroxyquinoline)- aluminum (AlQ) molecules, oriented using electric-field poling, and sandwiched between two Al/LiF electrodes is in excellent agreement with theory. This molecular device presents a new paradigm for a convenient, robust, inexpensive alternative to STM or mechanical break-junction structures.Comment: 5 pages, 3 figure

The use of rapid review methods in health technology assessments: 3 case studies.

BACKGROUND: Rapid reviews are of increasing importance within health technology assessment due to time and resource constraints. There are many rapid review methods available although there is little guidance as to the most suitable methods. We present three case studies employing differing methods to suit the evidence base for each review and outline some issues to consider when selecting an appropriate method. METHODS: Three recently completed systematic review short reports produced for the UK National Institute for Health Research were examined. Different approaches to rapid review methods were used in the three reports which were undertaken to inform the commissioning of services within the NHS and to inform future trial design. We describe the methods used, the reasoning behind the choice of methods and explore the strengths and weaknesses of each method. RESULTS: Rapid review methods were chosen to meet the needs of the review and each review had distinctly different challenges such as heterogeneity in terms of populations, interventions, comparators and outcome measures (PICO) and/or large numbers of relevant trials. All reviews included at least 10 randomised controlled trials (RCTs), each with numerous included outcomes. For the first case study (sexual health interventions), very diverse studies in terms of PICO were included. P-values and summary information only were presented due to substantial heterogeneity between studies and outcomes measured. For the second case study (premature ejaculation treatments), there were over 100 RCTs but also several existing systematic reviews. Data for meta-analyses were extracted directly from existing systematic reviews with new RCT data added where available. For the final case study (cannabis cessation therapies), studies included a wide range of interventions and considerable variation in study populations and outcomes. A brief summary of the key findings for each study was presented and narrative synthesis used to summarise results for each pair of interventions compared. CONCLUSIONS: Rapid review methods need to be chosen to meet both the nature of the evidence base of a review and the challenges presented by the included studies. Appropriate methods should be chosen after an assessment of the evidence base

Engineering of Yarrowia lipolytica for production of astaxanthin

Astaxanthin is a red-colored carotenoid, used as food and feed additive. Astaxanthin is mainly produced by chemical synthesis, however, the process is expensive and synthetic astaxanthin is not approved for human consumption. In this study, we engineered the oleaginous yeast Yarrowia lipolytica for de novo production of astaxanthin by fermentation. First, we screened 12 different Y. lipolytica isolates for β-carotene production by introducing two genes for β-carotene biosynthesis: bi-functional phytoene synthase/lycopene cyclase (crtYB) and phytoene desaturase (crtI) from the red yeast Xanthophyllomyces dendrorhous. The best strain produced 31.1 ± 0.5 mg/L β-carotene. Next, we optimized the activities of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG1) and geranylgeranyl diphosphate synthase (GGS1/crtE) in the best producing strain and obtained 453.9 ± 20.2 mg/L β-carotene. Additional downregulation of the competing squalene synthase SQS1 increased the β-carotene titer to 797.1 ± 57.2 mg/L. Then we introduced β-carotene ketolase (crtW) from Paracoccus sp. N81106 and hydroxylase (crtZ) from Pantoea ananatis to convert β-carotene into astaxanthin. The constructed strain accumulated 10.4 ± 0.5 mg/L of astaxanthin but also accumulated astaxanthin biosynthesis intermediates, 5.7 ± 0.5 mg/L canthaxanthin, and 35.3 ± 1.8 mg/L echinenone. Finally, we optimized the copy numbers of crtZ and crtW to obtain 3.5 mg/g DCW (54.6 mg/L) of astaxanthin in a microtiter plate cultivation. Our study for the first time reports engineering of Y. lipolytica for the production of astaxanthin. The high astaxanthin content and titer obtained even in a small-scale cultivation demonstrates a strong potential for Y. lipolytica-based fermentation process for astaxanthin production

Variability of Western Australian isolates of Sclerotinia sclerotiorum and the potential of Local Biological Control Agents

Stem rot disease caused by Sclerotinia sclerotiorum has emerged as a serious problem for canola (Brassica napus L.) production in Western Australia (WA) over the past few years where crop losses can be up to 40% in the worst affected crops. The biological characteristics and pathogenicity of the pathogen in WA is poorly understood. Also the potential for local biological control agents (BCAs) to be used in the management of the disease has not been explored. This paper provides preliminary data in these fields. One hundred and forty isolates of S. sclerotiorum were collected from WA canola growing regions for identification of biological characteristics which include colour of mycelia, growth rate, production of sclerotia, and pathogenicity. Other fungal isolates with potential biological control activity were collected from southern regions of WA. Colour of mycelia of Sclerotinia isolates varied from white, yellowish white, greyish white, brownish white, grey, dark grey to brown. Each isolate had its 24 and 48 hour growth rate recorded after sub-culture on PDA + ampicillin medium. ANOVA showed highly significant differences between growth rates of isolates 24 and 48 hours after being sub-cultured (P≤0.001). There were significant differences in number of sclerotia produced by each isolate. Two potential fungal biological control agents were found in WA, namely isolate KEN1 and isolate MTB1. These local fungal BCAs were found to be effective in inhibiting in vitro both the growth and ability to produce sclerotia of S. sclerotiorum

A plan to reduce adolescent suicide in Kern County

Adolescent suicide has been increasing at an alarming rate across the nation and within Kern County. The number of adolescent suicides in Kern County illustrates the need for the county to actively participate to remedy the existence of this problem. Kern County does have the proper mental health resources in the community, but they are fairly limited for adolescents. Moreover, there is a stigma associated with mental health, which can cause many adolescents to deter from seeking the necessary services. According to a review of the literature, adolescent suicide is a significant problem that is not just limited to Kern County. Suicide can stem from psychological issues, substance abuse, and environmental factors. Literature also suggests that there are certain populations more susceptible to committing suicide. Researchers suggest the use of public policy in areas, such as stricter firearm regulation and bullying to decrease the number of suicides. Additionally, researchers argue for a strong school based suicide prevention program. The recent statistics suggest the need for the development of a suicide prevention program in Kern County. There are a number of definitive warning signs and risk indicators for identifying potential suicide victims. This study offers a program plan to reduce adolescent suicide in Kern County; it is proposed that this be carried out by the Kern County Superintendent of Schools and the Kern County Mental Health Department. It will be created, as a no cost teaching program that is tailored to suit the specific needs of each different population. Individuals will be provided knowledge and tools to help prevent adolescent suicide through learning modules. This plan proposes a pilot program to ensure the highest quality program at the time of the rollout. Additionally, the paper recommends the creation of an accessible mobile application for adolescents, which will be created by KCSOS

Development of high-throughput methods to screen disease caused by Rhizoctonia solani AG 2-1 in oilseed rape

Background: Rhizoctonia solani (Kühn) is a soil-borne, necrotrophic fungus causing damping off, root rot and stem canker in many cultivated plants worldwide. Oilseed rape (OSR, Brassica napus) is the primary host for anastomosis group (AG) 2-1 of R. solani causing pre- and post-emergence damping-off resulting in death of seedlings and impaired crop establishment. Presently, there are no known resistant OSR genotypes and the main methods for disease control are fungicide seed treatments and cultural practices. The identification of sources of resistance for crop breeding is essential for sustainable management of the disease. However, a high-throughput, reliable screening method for resistance traits is required. The aim of this work was to develop a low cost, rapid screening method for disease phenotyping and identification of resistance traits. Results: Four growth systems were developed and tested: (1) nutrient media plates, (2) compost trays, (3) light expanded clay aggregate (LECA) trays, and (4) a hydroponic pouch and wick system. Seedlings were inoculated with virulent AG 2-1 to cause damping-off disease and grown for a period of 4–10 days. Visual disease assessments were carried out or disease was estimated through image analysis using ImageJ. Conclusion: Inoculation of LECA was the most suitable method for phenotyping disease caused by R. solani AG 2-1 as it enabled the detection of differences in disease severity among OSR genotypes within a short time period whilst allowing measurements to be conducted on whole plants. This system is expected to facilitate identification of resistant germplasm