In vitro pharmacological effects of Astragalus eremophilus and Melilotus parviflora

Traditional medicines are composed of herbal formulations and their active ingredients and constituents which play a crucial role in the treatment of various human ailments. Astragalus eremophilus and Melilotus indicus (L.) All. (syn. Melilotus parviflora Desf.) are used traditionally as antiperspirant, tonic, diuretic, laxative and narcotic agents. The current study was designed to investigate the Astragalus eremophilus and Melilotus indicus (L.) All. (syn. Melilotus parviflora Desf.) methanol extracts for their antioxidant, antibacterial and antifungal activities. Fine powder of A. eremophilus and M. parviflora was extracted with 70% methanol to get crude methanol extract. Extract was characterized for antioxidant, antibacterial and antifungal activities. Antioxidant activity of various concentrations (3 mg/ml, 1.5 mg/ ml, 0.75 mg/ml, and 0.38 mg/ml) of both plant extracts was analyzed using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical. Salmonella typhemorium, Klebsiella pneumoniae (gram-negative) and Staphylococcus aureus, Enterococcus faecalis (gram-positive) bacterial strains were used for assessment of antibacterial activities. Antifungal activities of 7.5 mg/ml, 5.0 mg/ml, 2.5 mg/ml (A. eremophilus and M. parviflora) were conducted using Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Candida albicons. At high concentration (3 mg/ml), all the tested fractions of A. eremophilus and M. parviflora methanol extracts showed potent antioxidant activities, ranging between 83.8 and 63.33%. Antibacterial activities revealed that A. eremophilus showed a maximum zone of inhibition (8.1 ± 0.1) on Salmonella typhenorium followed by Enterococcus faecalis (7.2 ± 0.1), Klebsellesa pneumonia (6.1 ± 0.6), and Staphylococcus aureus (5.1 ± 0.4), and at highest concentration (7.5 mg/ml), however, maximum zone of inhibition of Melilotus parviflora was at 7.5 mg/ml followed by 5.0 mg/ml and 2.5 mg/ml against Klebsiella pneumonia, Staphylococcus aureus, Salmonella typhemorium and Enterococcus faecalis. Antifungal assessment of both plant extracts showed that the higher concentration (7.5 mg/ml) has significant inhibitory effect as compared to control. The results can lead to the conclusion that A. eremophilus and M. parviflora methanol extracts are indeed sources of potential therapeutic compounds against antibacterial, antifungal and free radical associated disorders

Accelerated Proximal Algorithm for Finding the Dantzig Selector and Source Separation Using Dictionary Learning

In most of the applications, signals acquired from different sensors are composite and are corrupted by some noise. In the presence of noise, separation of composite signals into its components without losing information is quite challenging. Separation of signals becomes more difficult when only a few samples of the noisy undersampled composite signals are given. In this paper, we aim to find Dantzig selector with overcomplete dictionaries using Accelerated Proximal Gradient Algorithm (APGA) for recovery and separation of undersampled composite signals. We have successfully diagnosed leukemia disease using our model and compared it with Alternating Direction Method of Multipliers (ADMM). As a test case, we have also recovered Electrocardiogram (ECG) signal with great accuracy from its noisy version using this model along with Proximity Operator based Algorithm (POA) for comparison. With less computational complexity compared with ADMM and POA, APGA has a good clustering capability depicted from the leukemia diagnosis

Diversity of Mosquitoes Collected from the Southern Areas of Khyber Pakhtunkhwa Pakistan

OBJECTIVES: The objective of the study was to assess the diversity of mosquitoes in various towns of the southern belt of KPK. METHODOLOGY: This was a descriptive study that was conducted in numerous towns of Western belt of Khyber Pakhtunkhwa. The study areas were Darra Adam Khel, District Kohat, District Karak, District Banu, District D.I. Khan and newly merged districts Mir Ali and Miranshah. From each study site, the samples were collected randomly. The sample collection was done through survey and area visits whereas; the identification process was done in a parasitology laboratory of Hayatabad-Peshawar. Sampling was done from June 2016 to May 2017. RESULTS: A total of 2150 adult mosquitoes were gathered and collected from 42 different locations of the southern belt of KPK and were identified. Based on their identification, 5 genera of the mosquitoes were recognized which were Culex, Anopheles, Psorophora, Aedes and Uranotenia. Culex was found to be most dominant in all the visited areas with a percentage of 12.65 in Miranshah followed by 11.81 in Mirali, 7.16 in Karak, 6.88 in Darra Adam Khel, 6.69 in D.I.Khan, 6.41 in Kohat and 5.11 in Bannu respectively. The results of our findings also revealed the presence of Culex genera in all the habitats and remained the dominant genera among the others followed by Anopheles and Psorophora. Aedes was found in the habitat of plants and grasses etc. whereas Uranotenia was found in marsh/swampy areas as well as in plants/grasses habitat only. CONCLUSION: The outcomes reveal that a climate shifting and extensive urbanization process is enforcing the diversity of mosquitos’ fauna in the southern belt of KPK

Scenario of Manufacturing Pharmaceutical Small and Medium Enterprises(SMEs) in Pakistan

Small and medium enterprises of the whole world today, constitute more than 90% of the whole business entities throughout the world. Small and medium enterprises play a tremendous role in any economy like Pakistan as Pakistan is strategically located on the most important trade route between Asia and Europe. In Pakistan economy the manufacturing small and medium enterprises contribute more to the Pakistan’s economy. As the pharmaceutical industry in today’s world is an important and fundamental sector and is considered as a basic necessity in any locality of the globe. But one third of the whole population of the world at present do not access to the basic drugs of life. At the same time majority of the pharmaceutical industry of Pakistan comprise of small and medium enterprises and unfortunately face serious threats and simultaneously the empirical research in this sector is at initial stages

EFFECT OF JOB STRESS ON JOB SATISFACTION OF PHYSICAL EDUCATION TEACHERS WORKING IN GOVERNMENT COLLEGES

The current research is an attempt to examine the effect of job stress on job satisfaction of physical education teachers working in government colleges, Khyber Pakhtunkhwa (KP), Pakistan. A cross-sectional research method was used to collect required data from a finite population N=170 (males=97; females=73). Self-made questionnaires namely, Job Stress Questionnaire (JSQ) and Job Satisfaction Questionnaire (JSQ) were developed and used for the collection of required data. A statistical package for social sciences (SPSS), version; 26 was used to analyze the collected data, revealing negligible and inverse correlation (-.887, -.633 & -.721). The study revealed a significant impact of job stressors on job satisfaction (p < .05). Additionally, male physical education teachers reported a higher mean score on various variables included in the study (p < .05). These findings help policymakers to devise a uniform human policy that could protect the interests of the physical education teachers to share responsibilities of carving out the future of the country.  Â

Changing trends of Hepatitis B seromarkers amongst pakistani population: a laboratory-based review

Objective: To study the changing trends of hepatitis B markers tested at Aga Khan University Hospital clinical laboratory according to the internationally recognised classification of hepatitis B profile. Methods: The retrospective study involved analysis of laboratory records of hepatitis B profiles of all patients collected from January 2001 to December 2008 at the Aga Khan University Hospital\\u27s clinical laboratory. Patients with complete profile tested were categorised according to the Centre for Diseases Control classification of hepatitis B profile. SPSS 16 was used for statistical analysis. R Results: A total of 185,825 patients had serological markers for hepatitis B tested. Mean-age of reactive hepatitis B surface antigen (HBsAg) patients was 30±12.5 years. HBsAg reactivity was significantly higher in males than females (34% vs 12%; p Conclusion: The study substantiated the general perception that levels of HBsAg is showing a decreasing trend, while levels of HBsAb are increasing perhaps due to better vaccination of population

Reservoir Potential Evaluation of the Middle Paleocene Lockhart Limestone of the Kohat Basin, Pakistan: Petrophysical Analyses

The Lockhart Limestone is evaluated for its reservoir potential by utilizing wireline logs of Shakardara-01 well from Kohat Basin, Pakistan. The analyses showed 28.03% average volume of shale (Vsh), 25.57% average neutron porosity (NPHI), 3.31% average effective porosity (PHIE), 76% average water saturation (Sw), and 24.10% average hydrocarbon saturation (Sh) of the Lockhart Limestone in Shakardara-01 well. Based on variation in petrophysical character, the reservoir units of the Lockhart Limestone are divided into three zones i.e., zone-1, zone-2 and zone-3. Out of these zones, zone-1 and zone-2 possess a poor reservoir potential for hydrocarbons as reflected by very low effective porosity (1.40 and 2.02% respectively) and hydrocarbon saturation (15 and 5.20%), while zone-3 has a moderate reservoir potential due to its moderate effective porosity (6.50%) and hydrocarbon saturation (52%) respectively. Overall, the average effective porosity of 3.31% and hydrocarbon saturation of 24.10% as well as 28.03% volume of shale indicated poor reservoir potential of the Lockhart Limestone. Lithologically, this formation is dominated by limestone and shale interbeds in the Shakardara-01 well. Cross-plots of the petrophysical parameters versus depth showed that the Lockhart Limestone is a poor to tight reservoir in Shakardara-01 well and can hardly produce hydrocarbons under conventional drilling conditions

Interferon alpha for chronic Hepatitis D

Background: Hepatitis D virus is a small defective RNA virus that requires the presence of hepatitis B virus infection to infect a person. Hepatitis D is a difficult-to-treat infection. Several clinical trials have been published on the efficacy of interferon alpha for hepatitis D virus (HDV) infection. However, there are few randomised trials evaluating the effects of interferon alpha, and it is difficult to judge any benefit of this intervention from the individual trials. Objectives: To evaluate the beneficial and harmful effects of interferon alpha for Patients with chronic hepatitis D. Search methods: We identified relevant for the review randomised clinical trials by electronic searches in the Cochrane Hepato-Biliary Group Controlled Trials Register, the Cochrane Central Register of Controlled Trials (CENTRAL) in The Cochrane Library, MEDLINE, EMBASE, and Science Citation Index Expanded until May 2011. We also checked the bibliographic references of identified randomised trials, textbooks, and review articles in order to find randomised trials not identified by the electronic searches. Selection criteria: Randomised clinical trials evaluating interferon alpha versus placebo or no intervention for Patients with chronic hepatitis D infection. Data collection and analysis: Two authors assessed the trials and extracted data on mortality, virologic, biochemical, and histological response as well as adverse events at end of treatment and six months or more after completing treatment. The analyses were performed using the intention-to-treat principle including all randomised participants irrespective of follow-up. Drop-outs, withdrawals, and non-compliance were considered as treatment failures. Data were analysed with fixed- and random-effects models. Reported results were based on fixed-effect model except in cases where statistical significance varied between the two models. Main results: Six randomised trials fulfilled the inclusion criteria. Two hundred and one randomised participants (male = 174) were included. The risk of bias in all the included trials was high. Five trials compared interferon alpha with no treatment in the control group. One of these trials had two treatment arms with a higher dose and lower dose of interferon alpha and a no-treatment control group. We analysed both treatment regimens as a single group in a primary analysis and as separate groups in the subgroup analysis of different interferon dosages. The sixth trial compared only a higher dose of interferon alpha with a lower dose. Meta-analysis of five trials comparing interferon alpha with no-treatment control group included 169 participants. There were seven drop-outs in the treatment group and nine in the control group. One Patient out of 92 (1.1%) died in the interferon alpha group compared with zero out of 77 (0.0%) in the no-intervention control group (risk ratio (RR)) 3.00, 95% confidence interval (CI) 0.14 to 66.5). Interferon alpha led to failure of end of treatment virological response in 62/92 (67.4%) of the Patients compared with 71/77 (92.2%) in the untreated controls (RR 0.76, 95% CI 0.66 to 0.87, P = 0.0001 by fixed-effect model and RR 0.71, 95% CI 0.43 to 1.16, P = 0.17 by random-effects model). Failure of normalisation of alanine aminotransferase (ALT) at the end of treatment was seen in 60/92 (65.2%) Patients treated with interferon alpha versus 76/77 (98.7%) in the control group (RR 0.69, 95% CI 0.59 to 0.80, P \u3c 0.00001). Sustained virological response was not achieved in 76/92 (82.6%) of Patients on interferon compared with 73/77 (94.8%) of controls (RR 0.89, 95% CI 0.80 to 0.98, P = 0.02). Serum alanine aminotransferase was abnormal in 81/92 (88.0%) treated with interferon alpha Patients at six months post-treatment follow-up compared with 76/77 (98.7%) in controls (RR 0.92, 95% CI 0.84 to 0.99, P = 0.04). There was no significant histological improvement in 67/92 (72.8%) Patients treated with interferon alpha compared with 65/77 (84.4%) in controls (RR 0.86, 95% CI 0.74 to 1.00, P = 0.06). Two trials comparing a higher dose of interferon alpha with the lower dose showed no significant difference in sustained virological response (76.7% compared with 90.0%) (RR 0.85, 95% CI 0.68 to 1.07, P = 0.16). Adverse events such as flu-like symptoms, asthenia, weight loss, alopecia, thrombocytopenia, and leukopenia were reported in all these trials and the adverse events were related to interferon alpha. These were common and sometimes severe. One Patient in the treatment group was reported to have died by suicide towards the end of the study period. Authors\u27 conclusions: Interferon alpha does not seem to cure hepatitis D in most Patients. The agent seems effective in suppressing viral and liver disease activity in some Patients, but this improvement is not sustained in the majority of Patients. We cannot exclude overestimation of benefits and underestimation of harms due to high risk of bias (systematic errors) and high risk play of chance (random errors). Therefore, more randomised trials with large sample sizes and less risk of bias are needed before interferon can be recommended or refuted

Cognitive dual coprime frequency diverse array MIMO radar network for target discrimination and main-lobe interference mitigation.

The authors propose a novel dual coprime frequency diverse array (FDA) multiple input multiple output (DCFDA-MIMO) radar network design, empowered by cognitive capabilities, aimed at target discrimination and mitigation of interference present in the standalone radar systems. That is, the proposed DCFDA-MIMO design capitalises on the complementary advantages of FDAs for target discrimination and coprime arrays for enhanced resolution, resulting in superior performance. Additionally, the proposed DCFDA-MIMO network employs a 2D multiple signal classification algorithm to achieve high-resolution target localisation. By incorporating cognitive techniques based on the action-perception cycle, the proposed approach demonstrates notable improvements in multiple target detection and tracking accuracy with fewer number of antenna elements as compared to existing techniques. Furthermore, it enhances individual radar beamforming performance for interference suppression and true target detection without prior information

Yeast sludge and its components ameliorate ochratoxin Ainduced toxicity in broiler chicks

Purpose: To investigate the protective effect of yeast sludge (YS) and its components against ochratoxin A (OTA) in broiler chicks. Methods: Ochratoxin A (OTA) was produced through solid state fermentation, and quantified using high performance liquid chromatography (HPLC). A total of 1250 one-day old broiler chicks of Arbreaker breed were randomly assigned to five diet groups (A - E) replicated five times in such a way that each replicate had 50 chicks. The five diet groups were: A (normal poultry feed), B (200 µg/kg OTA), C (200 µg/kg OTA plus 2 g/kg YS), D (200 µg/kg OTA plus 2 g/kg yeast sludge cell mass, YSCM), and E (200 µg/kg OTA plus 2 g/kg yeast sludge cell wall (YSCW). The study lasted 35 days. Indices of liver and kidney functions were determined, as well as histopathological examination of samples of kidney, liver and bursa of Fabricius. Results: Supplementation of chicks diet with OTA at a dose of 200 ppb significantly reduced serum levels of total protein (TP), albumin, and creatinine, but significantly raised the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) (p < 0.05). However, inclusion of YS, YSCM, and YSCW in OTA-contaminated diet significantly reversed the effect of OTA on the indices of liver and kidney functions (p < 0.05). Yeast sludge and its components also significantly ameliorated OTA-induced histological changes in the liver, kidney and bursa of Fabricius. Conclusion: These results indicate that YS and it components improve hepatorenal function and histological changes induced by OTA