Pulmonary dysfunction in advanced liver disease

PURPOSE: Abnormalities in pulmonary function have been reported in association with chronic liver disease of varied etiology. The aim of this study was to better define the frequency and nature of these abnormalities in patients who were being evaluated for liver transplantation. PATIENTS AND METHODS: We performed a battery of pulmonary function tests and chest radiographs in 116 consecutive patients (50 men, 66 women; aged 19 to 70 years, mean 44.6 years) with severe advanced liver disease who were hospitalized specifically for evaluation for possible orthotopic liver transplantation and were able to perform technically satisfactory tests. In 17 patients, quantitative whole-body technetium-99m macroaggregated albumin perfusion scanning was also performed for assessment of possible right-to-left shunting through intrapulmonary vascular dilatations.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/38372/1/1840150231_ftp.pd

The Geostationary Carbon Process Mapper

The Geostationary Carbon Process Mapper (GCPM) is an earth science mission to measure key atmospheric trace gases and process tracers related to climate change and human activity. The measurement strategy delivers a process based understanding of the carbon cycle that is accurate and extensible from city to regional and continental scales. This understanding comes from contiguous maps of carbon dioxide (CO2), methane (CH4), carbon monoxide (CO), and chlorophyll fluorescence (CF) collected up to 10 times per day at high spatial resolution (~4km × 4km) from geostationary orbit (GEO). These measurements will capture the spatial and temporal variability of the carbon cycle across diurnal, synoptic, seasonal and interannual time scales. The CO2/CH4/CO/CF measurement suite has been specifically selected because their combination provides the information needed to disentangle natural and anthropogenic contributions to atmospheric carbon concentrations and to minimize key uncertainties in the flow of carbon between the atmosphere and surface since they place constraints on both biogenic uptake and release as well as on combustion emissions. Additionally, GCPM's combination of high-resolution mapping and high measurement frequency provide quasi-continuous monitoring, effectively eliminating atmospheric transport uncertainties from source/sink inversion modeling. GCPM uses a single instrument, the “Geostationary Fourier Transform Spectrometer (GeoFTS)” to make measurements in the near infrared spectral region at high spectral resolution. The GeoFTS is a half meter cube size instrument designed to be a secondary “hosted” payload on a commercial GEO satellite. NASA and other government agencies have adopted the hosted payload implementation approach because it substantially reduces the overall mission cost. This paper presents a hosted payload implementation approach for measuring the major carbon-containing gases in the atmosphere from the geostationary vantage point, to affordably advance the scientific understating of carbon cycle processes and climate change

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

A cellulose-based photoacoustic sensor to measure heparin concentration and activity in human blood samples

Heparin is an indispensable drug in anticoagulation therapy but with a narrow therapeutic window, which dictates regular testing and dose adjustment. However, current monitoring tools have a long turnaround time or are operator intensive. In this work, we describe a cellulose-based photoacoustic sensor for heparin. The sensors have a turnaround time of 6 min for whole blood samples and 3 min for plasma samples regardless of heparin concentration. These sensors have a limit of detection of 0.28 U/ml heparin in human plasma and 0.29 U/ml in whole blood with a linear response (Pearson's r = 0.99) from 0 to 2 U/ml heparin in plasma and blood samples. The relative standard deviation was < 12.5% in plasma and < 17.5% in whole blood. This approach was validated with heparin-spiked whole human blood and had a linear correlation with the activated partial thromboplastin time (aPTT) (r = 0.99). We then studied 16 sets of clinical samples-these had a linear correlation with the activated clotting time (ACT) (Pearson's r = 0.86, P < 0.0001). The photoacoustic signal was also validated against the cumulative heparin dose (Pearson's r = 0.71, P < 0.0001). This approach could have applications in bed-side heparin assays for continuous heparin monitoring